FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685167

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685167
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1146077
Sequences flagged as poor quality0
Sequence length18-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTAGACTGCTCATTTATTGGGCGTCTACCCGGGAATCCGGGGTCCCAGACCGATTCAGTGGCAGCGGGTCTG77710.678052172759771No Hit
GTCTAGGAGGCTGGCCTAATTTTTGCTGGTACCAAGCTAAGGCGTTCTTATTGGTGGACCTATCTAAAAGACT72460.63224373231467No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT52030.4539834583540198No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTA50170.43775418231061264No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG45370.39587217961794885No Hit
CTTTTAGATAGGTCCACCAATAAGAACGCCTTAGCTTGGTACCAGCAAAA40160.3504127558619534No Hit
GTCCCAGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCACCATCAACAGCCTGCAGACTGAAGAT37050.32327670828399835No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTT35820.3125444450940033No Hit
GGATTATAATATTGCTGACAGTAATAAATCGCCACATCTTCAGTCTGCAGGCTGTTGATGGTGAGAGTGAAAT34820.303819027866365No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC34500.30102689435352076No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGC33330.29081815619718393No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG30380.26507817537565104No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTC29590.2581850957658168No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCC28120.2453587324411885No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT27390.23898917786501256No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGTTA27350.23864016117590703No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT26620.2322706065997311No Hit
TTATAATCCTCCGTGGACATTCGGCCAAGGGACCAAGGTGGAAATGAAACGAACTGTGGCTGCACCATCTGTCTT26350.22991474394826872No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAA26220.22878043970867576No Hit
GTCTGGGACCCCGGATTCCCGGGTAGACGCCCAATAAATGAGCAGTCTAGGAGGCTGGCCTAATTTTTGCTGGTA25190.21979325996420834No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGCTGCTCTGGATCTCTGGTAC24960.21778641400185153No Hit
ATTATAATCCTCCGTGGACATTCGGCCAAGGGACCAAGGTGGAAATGAAA23660.20644337160592177No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACT23150.20199340881982628No Hit
ATATTATAATCCTCCGTGGACATTCGGCCAAGGGACCAAGGTGGAAATGAAACGAACTGTGGCTGCACCAT22380.19527483755454475No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT22140.19318073741991157No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGA21830.1904758580793437No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT21200.18497884522593158No Hit
GTCCCAGACCCGCTGCCACTGAATCGGTCTGGGACCCCGGATTCCCGGGTAGACGCCCAATAAATGAGCAGTCTA20470.17860929064975564No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA19950.17407207369138372No Hit
GATTTCACTCTCACCATCAACAGCCTGCAGACTGAAGATGTGGCGATTTATTACTGTCAGCAATATTATAATCCT19910.1737230570022782No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACA18660.16281628546773036No Hit
GAGTGAAATCTGTCCCAGACCCGCTGCCACTGAATCGGTCTGGGACCCCG18590.16220550626179567No Hit
GTGCAGCCACAGTTCGTTTCATTTCCACCTTGGTCCCTTGGCCGAATGTC18510.1615074728835846No Hit
ATCTAAAAGACTCTGGCTGGACCTGCAGTTGACGGTGGTTCTCTCGCCCAGAGACACAGCCAGGGAGCCTGGA18350.1601114061271625No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC18010.15714476426976548No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT17840.15566144334106696No Hit
CCCTGGCTGTGTCTCTGGGCGAGAGAACCACCGTCAACTGCAGGTCCAGC17410.1519095139331825No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT17090.14911738042033823No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTCATTTCCACCTTGGTCCCTTGGCCGAATGTCCACGGAGGATT16460.14362036756692612No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCT16330.14248606332733316No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG15830.138123354713514No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT15660.1366400337848155No Hit
GGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGCTGCTCTGGATCTCTGGTACTTACGGT15590.13602925457888082No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGG15470.13498220451156423No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAA15440.1347204419947351No Hit
GTCTCCAGGCTCCCTGGCTGTGTCTCTGGGCGAGAGAACCACCGTCAACTGCAGGTCCAGCCAGAGTCTTTTA15270.13323712106603658No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC15210.13271359603237828No Hit
GTCTGGGGCTGACGTGAAGAAGCCTGGGGCCTCAGTGAAGGTTTCCTGCAAGGCGTCTGGATACAGCTTCACCA15170.13236457934327275No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA14900.1300087166918104No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC14790.1290489207967702No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTA14750.12869990410766466No Hit
GTTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCACTGAATCGGTCTGGGACCCCGGATTCCCGGGTA14710.12835088741855913No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGACTTGCCGGCTAGGCACTGTGTGGCC14700.12826363324628276No Hit
GTATCAACGCAGAGTACGGGGAGCTACAACAGGCAGGCAGGGGCAGCAAG14590.12730383735124254No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA14380.12547149973343852No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA14350.12520973721660936No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA14250.12433719549384552No Hit
GTAATAAATCGCCACATCTTCAGTCTGCAGGCTGTTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCA14220.12407543297701638No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCA14190.12381367046018722No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTAC14180.12372641628791085No Hit
TTATAATATTGCTGACAGTAATAAATCGCCACATCTTCAGTCTGCAGGCTGTTGATGGTGAGAGTGAAATCTGTC14060.12267936622059425No Hit
CACCATCAACAGCCTGCAGACTGAAGATGTGGCGATTTATTACTGTCAGCAATATTATAATCCTCCGTGGACATT13980.12198133284238319No Hit
GCTCTGGATCTCTGGTACTTACGGTGACATCGTGATGACCCAGTCTCCAGGCTCCCTGGCTGTGTCTCTGGGCGA13910.12137055363644852No Hit
GGACCAAGGTGGAAATGAAACGAACTGTGGCTGCACCATCTGTCTTCATC13810.12049801191368469No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG13680.1193637076740917No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG13580.11849116595132787No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGAT13560.11831665760677511No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT13540.11814214926222234No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC13520.11796764091766958No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTTCCATGGCTCGGCACAGCCCGGCAG13480.11761862422856403No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAG13470.11753137005628767No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC13130.11456472819889064No Hit
CAGTAATAAATCGCCACATCTTCAGTCTGCAGGCTGTTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCA13100.11430296568206151No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA12890.11247062806425745No Hit
CTGCAGGTCCAGCCAGAGTCTTTTAGATAGGTCCACCAATAAGAACGCCTTAGCTTGGTACCAGCAAAAATTAG12750.1112490696523881No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA12610.11002751124051875No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAACGTGGTCATCGCCTGCCTGGTCCAGG12460.108718698656373No Hit
AGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGCTGCTCTGGATCTCTGGTACTTACGGTGACATCGTGAT12410.10828242779499109No Hit
ATTTATTACTGTCAGCAATATTATAATCCTCCGTGGACATTCGGCCAAGGGACCAAGGTGGAAATGAAACGAA12380.10802066527816194No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT12360.10784615693360917No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA12310.10740988607222726No Hit
GGCTGTGTCTCTGGGCGAGAGAACCACCGTCAACTGCAGGTCCAGCCAGAGTCTTTTAGATAGGTCCACCAATAA12190.10636283600491067No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTT12000.10470500673165938No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC11890.10374521083661918No Hit
GCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGCTGCTCTGGATCTCTGGTACTTACGGTGACATCGT11850.10339619414751365No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTCGTTTCATTTCCACCTTG11820.1031344316306845No Hit
GAATCGGTCTGGGACCCCGGATTCCCGGGTAGACGCCCAATAAATGAGCAGTCTAGGAGGCTGGCCTAATTTTT11780.10278541494157897No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGT11760.10261090659702621No Hit
CCTTGGTCCCTTGGCCGAATGTCCACGGAGGATTATAATATTGCTGACAGTAATAAATCGCCACATCTTCAGT11680.10191287321881515No Hit
TCTTCAGTCTGCAGGCTGTTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCACTGAATCGGTCTGGGACC11540.10069131480694579No Hit
TTATTACTGTCAGCAATATTATAATCCTCCGTGGACATTCGGCCAAGGGACCAAGGTGGAAATGAAACGAACTGT11490.10025504394556387No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCGAA850.00639996353.59796570
TAGGCAA1153.1863505E-452.82118270
TAAAACG200.007404292551.66974646
GTACCGA200.00789500650.8378526
CTGCTAA900.00802289450.62029670
CTTGGGT900.00802289450.62029670
GTTATTA1203.9311755E-450.62029670
AATGGTA1552.3507799E-548.98738570
ACAAGCA950.00993378447.95607470
GTGGTAT1350.047.7434581
GGTATCA7300.047.3992421
GTTTAAA1305.833869E-446.72643370
TGGTATC1450.044.419772
GTATCAA21900.042.9071581
GAGCAAA27850.040.3508370
GTTGATA1959.1128866E-538.93869470
GCGAGAT350.001461169238.78606821
AAGGACA29250.037.9003370
TATCAAC25150.037.471192
AAGCGGT8600.037.08231470
Produced by FastQC (version 0.11.5)