FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004685168

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685168
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences830090
Sequences flagged as poor quality0
Sequence length18-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTGGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAG27090.3263501548024913No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC25280.30454529026972976No Hit
GTAATATACAGCCGTGTCTTCAGATCTGAGGCTGCTCAATTCCATGTAGACTGTTCTCGCGGATGTGTCCCTGG24690.2974376272452385No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTGG21360.25732149525955017No Hit
GTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC19940.24021491645484222No Hit
GACTATTACTGTCAATCAGCTGACAGCAACAATAATCTTAGAGGCTTCGG19910.2398535098603766No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACGGTGACCTTGGTCCCAG19520.23515522413232298No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC18890.22756568564854412No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC16800.2023876929007698No Hit
GTATATTACTGTGCGAGGGAGGGGGGTGACTTTGGTGAACCAGGCTTCGA16370.19720753171342864No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT16000.19275018371501884No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCT15920.19178643279644375No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGG15030.18106470382729584No Hit
CTATTACTGTCAATCAGCTGACAGCAACAATAATCTTAGAGGCTTCGGAACTGGGACCAAGGTCACCGTCCTAGG15020.18094423496247394No Hit
ATATAGGGGCCTGGCCTGGCTTCTGTTGGTACCAGTAAGCATATTGCTTTGACAATGCATCTGCAGAGCAGGTGA14770.17793251334192678No Hit
GACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGCCTCTAAGATTATTG14770.17793251334192678No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG13650.16444000048187546No Hit
ACACTGGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC13290.16010312134828755No Hit
GCCTTGGGCTGACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGCCTCTAAGATTATTGTTGCTGTCAGCTGAT12980.15636858653880903No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT12850.1548024912961245No Hit
TCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGG12660.15251358286450867No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATGGGTGGGTTTACCCGCCAAGCGG12360.14889951691985207No Hit
GTAATAGTCAGCCTCGTCTTCTGCCTGGACTCCACTGATGGTCAACGTGA12180.1467310773530581No Hit
CAATAAGAGGCCCTCAGGGATCCCTGAGCGATTCTCTGGCTCCAGCTCAGGGACAACGGTCACGTTGACCATCAG11950.14396029346215472No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG11720.14118950957125131No Hit
ACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGCCTCTAAGATTATTGT11520.13878013227481356No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC11490.1384187256803479No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGC11450.13793685022106036No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGCCTCTAAGATTAT11300.13612981724873205No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGC11040.13299762676336302No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTGGTGTGTCTGATCAGTGACT10840.13058824946692527No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT10660.1284198099001313No Hit
CACCAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG10580.1274560589815562No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGT10520.1267332457926249No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG10150.12227589779421509No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA9910.11938464503848979No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGA9560.11516823476972375No Hit
CCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGG9530.1148068281752581No Hit
GTCCAGGAGGCAGAACTCTGGGTGTCTCACCATGGCCTGGATCCCTCTAC9520.11468635931043622No Hit
GGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTGGA9430.11360213952703925No Hit
CTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCCCG9310.1121565131491766No Hit
CCCGTGGGTGGGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCA9280.11179510655471092No Hit
CCACCAAACCCTCCAAACAGAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCTGACGCCCGAGCAG9170.11046994904167018No Hit
CTCTCTGCACAGACTCTGAGGCCTCCTTTGAGCTGACACAGCCACCCTCGGTGTCAGTGTCCCCAGGACAGACGG9140.11010854244720451No Hit
GACAATAAGAGGCCCTCAGGGATCCCTGAGCGATTCTCTGGCTCCAGCTC9130.10998807358238262No Hit
CCCCAGGACAGACGGCCAGGATCACCTGCTCTGCAGATGCATTGTCAAAG9010.10854244720452No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAGTGTCCTGCCGGGCTGTGCCGAGCCA8860.10673541423219168No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTGGTGT8820.10625353877290414No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG8700.1048079123950415No Hit
ACCTGAGCCTGACGCCCGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCACCG8610.10372369261164452No Hit
TCAGTGGAGTCCAGGCAGAAGACGAGGCTGACTATTACTGTCAATCAGCTGACAGCAACAATAATCTTAGAGGCT8550.1030008794227132No Hit
GGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGT8490.10227806623378187No Hit
CATTGACATGGGTGGGTTTACCCGCCAAGCGGTCGATGGTCTTCTGTGTGAAGGCCAGCGGCAGGGCCTCGTGGC8320.10023009553180981No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)