FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685169

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685169
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences830090
Sequences flagged as poor quality0
Sequence length18-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC26260.3163512390222747No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTGGTGTGTCTGATCAGT25620.30864123167367397No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG24650.2969557517859509No Hit
GTAATATACAGCCGTGTCTTCAGATCTGAGGCTGCTCAATTCCATGTAGACTGTTCTCGCGGATGTGTCCCTGGT23730.28587261622233734No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTGGT18630.22443349516317507No Hit
GACTATTACTGTCAATCAGCTGACAGCAACAATAATCTTAGAGGCTTCGGAACTGGGACCAAGGTCACCGTCCTA18220.21949427170547772No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC17470.2104591068438362No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC17320.20865207387150791No Hit
GTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAG16650.20058065992844148No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA16560.1994964401450445No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT15300.18431736317748676No Hit
GTATATTACTGTGCGAGGGAGGGGGGTGACTTTGGTGAACCAGGCTTCGA14670.1767278246937079No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTA14520.17492079172137961No Hit
ATATAGGGGCCTGGCCTGGCTTCTGTTGGTACCAGTAAGCATATTGCTTTGACAATGCATCTGCAGAGCAGGT14450.1740775096676264No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGT14400.17347516534351698No Hit
CTATTACTGTCAATCAGCTGACAGCAACAATAATCTTAGAGGCTTCGGAA14380.1732342276138732No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT13840.16672890891349132No Hit
ACACTGGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC13120.15805515064631545No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTGGTGTGTCTGATCAGTGACTTCTACCCGG11740.1414304473008951No Hit
CAATAAGAGGCCCTCAGGGATCCCTGAGCGATTCTCTGGCTCCAGCTCAG11390.13721403703212906No Hit
GACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGCCTCTAAGATTATTG11210.1350455974653351No Hit
ACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGCCTCTAAGATTATTGTTGCTGTCAGCTGATTGACAGTAATA11160.13444325314122565No Hit
GCCTTGGGCTGACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGCCTCT11160.13444325314122565No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT10800.13010637400763772No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC10770.12974496741317207No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACGGTGACCTTGGTC10640.12817887217048754No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCT10570.12733559011673434No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG10510.126612776927803No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTT10500.12649230806298112No Hit
TCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAG10380.12504668168511848No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTG10330.12444433736100904No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC10270.12372152417207774No Hit
CACCAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGG10250.12348058644243394No Hit
GGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCCCGTCAAGGC9910.11938464503848979No Hit
GTAATAGTCAGCCTCGTCTTCTGCCTGGACTCCACTGATGGTCAACGTGACCGTTGTCCCTGAGCTGGAGCCAGA9780.11781854979580528No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG9690.11673433001240828No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC9650.11625245455312075No Hit
GGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGC9550.11504776590490187No Hit
CCCCAGGACAGACGGCCAGGATCACCTGCTCTGCAGATGCATTGTCAAAG9390.11312026406775169No Hit
GACAATAAGAGGCCCTCAGGGATCCCTGAGCGATTCTCTGGCTCCAGCTC9060.10914479152862942No Hit
GTCCAGGAGGCAGAACTCTGGGTGTCTCACCATGGCCTGGATCCCTCTAC8850.10661494536736979No Hit
CCCGTGGGTGGGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCA8810.10613306990808226No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTTCCATGGCTCGGCACAGCCCGGCAG8580.10336228601717885No Hit
CCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGG8530.10275994169306943No Hit
CTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCCCG8510.10251900396342564No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT8470.10203712850413811No Hit
ACCTGAGCCTGACGCCCGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC8470.10203712850413811No Hit
GTACCTGACTTGGGCATCCCGGCAGGAGCCCAGCCAGGGCACCACCACCTTCGCTGTGACCAGCATACTGCGCGT8360.10071197099109734No Hit
CTCTCTGCACAGACTCTGAGGCCTCCTTTGAGCTGACACAGCCACCCTCGGTGTCAGTGTCCCCAGGACAGACG8320.10023009553180981No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGCGT705.362478E-575.72047470
CGTGGTT700.00508934256.79035670
GTAGTTT1702.6437192E-854.56328670
AATGGTA1753.322566E-853.00433370
TAGGCAA1251.4739022E-553.00433370
CCGCTAT1352.3238648E-549.07808770
TTTCCTA1156.2389136E-446.0907270
GGTATCA7500.043.4650231
GTATCAA22400.041.688341
GAGCCAT9500.036.2661270
AACACAA5900.035.9351470
ATCAACG26900.034.7966083
TCAACGC27300.034.4109954
GCTGATT11200.034.3108470
GCACCGT10050.034.2814170
TCTGTCG400.002825011333.918
AACGCAG27950.033.7300456
CAACGCA27900.033.668955
TATCAAC28250.033.261832
CGCAGAG29100.032.2785878
Produced by FastQC (version 0.11.5)