FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004685172

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685172
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1012327
Sequences flagged as poor quality0
Sequence length19-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGG53110.5246328508476016No Hit
GTCTCTGTGGCCTCCTATGAGTTGACACAGCCATCCTCAGTGTCAGTGTCTCCGGGACAGACAGTCAGGATCAC46840.46269634218982597No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAGACCCCATTCTGGTCAGCCGCAGAGTAACAG42800.4227882887644013No Hit
GGAAATATACTGGCAGAAAAATATGTTCGGTGGTTCCAGCAGAAGCCAGG42680.4216029010388936No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG39960.39473411259405317No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAG37420.3696434057374741No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAGACCCCATTCTGGTCAGCCGCAGAGTAACAGTAATAGTCAG33380.32973535231204937No Hit
CTATTACTGTTACTCTGCGGCTGACCAGAATGGGGTCTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCC32360.3196595566452342No Hit
CAGTAATAGTCAGCCTCATCCTCAACCTGGGCCCCGGTGATGGTCAAGGT31840.3145228765013676No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC31570.31185575411897537No Hit
GTGTCAGTGTCTCCGGGACAGACAGTCAGGATCACCTGCTCAGGAAATATACTGGCAGAAAAATATGTTCGGTGG31560.3117569718085164No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCT29920.295556672893245No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG28980.2862711357101016No Hit
GAGTAACAGTAATAGTCAGCCTCATCCTCAACCTGGGCCCCGGTGATGGTCAAGGTGACTGTGGTCCCTGAGC28860.2850857479845939No Hit
CCTATGAGTTGACACAGCCATCCTCAGTGTCAGTGTCTCCGGGACAGACAGTCAGGATCACCTGCTCAGGAAATA27350.2701696191052891No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT26550.26226703426857134No Hit
GAATGGGGTCTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGT26210.2589084357129663No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC25440.2513021978076254No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC24370.24073249058851534No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG24290.2399422321048436No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC23510.23223721188904375No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG23200.22917496026481562No Hit
GCCATCCTCAGTGTCAGTGTCTCCGGGACAGACAGTCAGGATCACCTGCTCAGGAAATATACTGGCAGAAAAATA23080.22798957253930796No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC22600.22324802163727728No Hit
GTAATAGACAGCCGTGTCCTCGGGTCTCAGGCTGTTCATTTGCAGAAACA22050.2178149945620338No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG21800.2153454368005595No Hit
GACTATTACTGTTACTCTGCGGCTGACCAGAATGGGGTCTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAG21220.20961606279393913No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA21150.2089245866207263No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGG20330.2008244371630906No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC20260.2001329609898778No Hit
CTGTTACTCTGCGGCTGACCAGAATGGGGTCTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGG20150.19904635557482908No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA20070.1982560970911573No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGC19920.19677436243427274No Hit
GCTCTGAATGTCTCACCATGGCCTGGATCCCTCTCCTGCTCCCCCTCCTCATTCTCTGCACAGTCTCTGTGGCC19770.19529262777738815No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG19630.19390967543096255No Hit
GGTTGAGGATGAGGCTGACTATTACTGTTACTCTGCGGCTGACCAGAATGGGGTCTTCGGCGGAGGGACCAAG19380.19144011766948824No Hit
GTATATTTCCTGAGCAGGTGATCCTGACTGTCTGTCCCGGAGACACTGAC18970.18739004294067035No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA18530.18304362128047558No Hit
TCCTCATTCTCTGCACAGTCTCTGTGGCCTCCTATGAGTTGACACAGCCA18500.18274727434909865No Hit
CAGTCACCTTGACCATCACCGGGGCCCAGGTTGAGGATGAGGCTGACTAT18330.18106797507129613No Hit
GTAATAGTCAGCCTCATCCTCAACCTGGGCCCCGGTGATGGTCAAGGTGA18100.17879598193073978No Hit
CTCCGGGACAGACAGTCAGGATCACCTGCTCAGGAAATATACTGGCAGAA17360.17148609095677583No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT17120.1691153155057605No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC17070.16862140395346562No Hit
GCTCAGGAGGCAGAGCTCTGAATGTCTCACCATGGCCTGGATCCCTCTCC17000.1679299277802528No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGT16880.16674454005474515No Hit
GCTCTAGTCTCCCGTGGTGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGG16730.1652628053978606No Hit
GCCCTCTGGGATCCCTGAGCGCTTCTCCGGCTCCAGCTCAGGGACCACAG16730.1652628053978606No Hit
CCAATAGAGGGGCCTGGCCTGGCTTCTGCTGGAACCACCGAACATATTTTTCTGCCAGTATATTTCCTGAGCAGG16670.16467011153510674No Hit
TCCTCAACCTGGGCCCCGGTGATGGTCAAGGTGACTGTGGTCCCTGAGCTGGAGCCGGAGAAGCGCTCAGGGATC16630.16427498229327084No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCTGACGCCTGAGCAG16530.1632871591886811No Hit
CTATTGGTGATTTATAAGGGCAGCGAGCGGCCCTCTGGGATCCCTGAGCG16520.16318837687822216No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAGACCCCATTCTGGTCAGCCGCAGAGTAACAGTAATAGTCAGC16120.15923708445986326No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT16010.15815047904481458No Hit
AGGTGACTGTGGTCCCTGAGCTGGAGCCGGAGAAGCGCTCAGGGATCCCAGAGGGCCGCTCGCTGCCCTTATAAA15770.15577970359379925No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA15580.15390283969507876No Hit
GTCTATTACTGTACGCGAGAGGCTAAACATTTCGGTGACTTCGATTTTGA15230.15044545882901475No Hit
TCTCTGCACAGTCTCTGTGGCCTCCTATGAGTTGACACAGCCATCCTCAGTGTCAGTGTCTCCGGGACAGACAG14850.1466917310315738No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAGACCCCAT14840.1465929487211148No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAGACCCCATTCTGG14630.1445185202014764No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG14570.14392582633872258No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCT14460.14283922092367388No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGTGAGGGTTGAG14090.1391842754366919No Hit
GGATGAGGCTGACTATTACTGTTACTCTGCGGCTGACCAGAATGGGGTCTTCGGCGGAGGGACCAAGCTGACCG14060.13888792850531498No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCACCG13540.13375124836144842No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC13360.13197316677318693No Hit
CCTCATTCTCTGCACAGTCTCTGTGGCCTCCTATGAGTTGACACAGCCAT13170.13009630287446644No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG13170.13009630287446644No Hit
TCCCTCTCCTGCTCCCCCTCCTCATTCTCTGCACAGTCTCTGTGGCCTCCTATGAGTTGACACAGCCATCCTCAG13150.1298987382535485No Hit
CCCGTGGTGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGCCA13150.1298987382535485No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT13140.12979995594308952No Hit
GAGTTGACACAGCCATCCTCAGTGTCAGTGTCTCCGGGACAGACAGTCAGGATCACCTGCTCAGGAAATATAC13050.12891091514895878No Hit
ACCCCATTCTGGTCAGCCGCAGAGTAACAGTAATAGTCAGCCTCATCCTC12780.12624379276656653No Hit
GGCCTGGATCCCTCTCCTGCTCCCCCTCCTCATTCTCTGCACAGTCTCTG12710.12555231659335372No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTG12680.1252559696619768No Hit
CGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACT12590.12436692886784606No Hit
GTATCAACGCAGAGTACGGGGTAGGCTCAGGAGGCAGAGCTCTGAATGTCTCACCATGGCCTGGATCCCTCTC12510.12357667038417428No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA12450.12298397652142044No Hit
ACCTGGGCCCCGGTGATGGTCAAGGTGACTGTGGTCCCTGAGCTGGAGCC12390.12239128265866663No Hit
GCTTGGTCCCTCCGCCGAAGACCCCATTCTGGTCAGCCGCAGAGTAACAGTAATAGTCAGCCTCATCCTCAAC12390.12239128265866663No Hit
GGCCAGGCCCCTCTATTGGTGATTTATAAGGGCAGCGAGCGGCCCTCTGG12260.12110711262269998No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA12210.12061320107040512No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG12150.1200205072076513No Hit
CTCCTGCTCCCCCTCCTCATTCTCTGCACAGTCTCTGTGGCCTCCTATGA12120.11972416027627437No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGT12110.1196253779658154No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT12090.11942781334489745No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC12030.11883511948214362No Hit
GGATCACCTGCTCAGGAAATATACTGGCAGAAAAATATGTTCGGTGGTTCCAGCAGAAGCCAGGCCAGGCCCC11890.11745216713571802No Hit
CAGTAATAGACAGCCGTGTCCTCGGGTCTCAGGCTGTTCATTTGCAGAAACAACGTGTTCTTGGAATTGTCTC11840.11695825558342314No Hit
AGCCAGGCCAGGCCCCTCTATTGGTGATTTATAAGGGCAGCGAGCGGCCC11830.11685947327296417No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG11710.11567408554745649No Hit
GGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG11660.11518017399516164No Hit
GTCAGGATCACCTGCTCAGGAAATATACTGGCAGAAAAATATGTTCGGTGGTTCCAGCAGAAGCCAGGCCAGGCC11130.10994471154083611No Hit
GGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAG11050.10915445305716433No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTAGGTGGGAAGTTTCTGGCGGT11000.10866054150486947No Hit
CTGCACAGTCTCTGTGGCCTCCTATGAGTTGACACAGCCATCCTCAGTGT10960.10826541226303357No Hit
TTCTCTGCACAGTCTCTGTGGCCTCCTATGAGTTGACACAGCCATCCTCAGTGTCAGTGTCTCCGGGACAGACAG10790.10658611298523105No Hit
GCCTCATCCTCAACCTGGGCCCCGGTGATGGTCAAGGTGACTGTGGTCCC10760.10628976605385414No Hit
CTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCC10710.10579585450155929No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACT10690.10559828988064135No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAGTGTCCTGCCTGGCTGTGCCCAGCCA10640.10510437832834647No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC10590.10461046677605161No Hit
GATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGG10350.10223969132503628No Hit
GGGTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG10340.1021409090145773No Hit
GCACAGTCTCTGTGGCCTCCTATGAGTTGACACAGCCATCCTCAGTGTCAGTGTCTCCGGGACAGACAGTCAGGA10250.10125186822044654No Hit
GACCATCACCGGGGCCCAGGTTGAGGATGAGGCTGACTATTACTGTTACTCTGCGGCTGACCAGAATGGGGTCT10140.10016526280539786No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)