FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685173

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685173
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1012327
Sequences flagged as poor quality0
Sequence length18-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGT49890.49282494687981254No Hit
GTCTCTGTGGCCTCCTATGAGTTGACACAGCCATCCTCAGTGTCAGTGTCTCCGGGACAGACAGTCAGGATCAC43870.4333579959835112No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG39250.3877205685514661No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAGACCCC38450.3798179837147483No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT36340.35897491620790517No Hit
GGAAATATACTGGCAGAAAAATATGTTCGGTGGTTCCAGCAGAAGCCAGG34260.3384281956324389No Hit
CTATTACTGTTACTCTGCGGCTGACCAGAATGGGGTCTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCC31860.3147204411222856No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAGACCCCATTCTGGTCAGCCGCAGAGTAACAGTAATAGTCA31330.30948497866796004No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG30300.29931040069068593No Hit
GTGTCAGTGTCTCCGGGACAGACAGTCAGGATCACCTGCTCAGGAAATAT29530.29170416278534506No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC27930.27589899311190946No Hit
CAGTAATAGTCAGCCTCATCCTCAACCTGGGCCCCGGTGATGGTCAAGGTGACTGTGGTCCCTGAGCTGGAGCCG27530.2719477006935506No Hit
GAGTAACAGTAATAGTCAGCCTCATCCTCAACCTGGGCCCCGGTGATGGTCAAGGTGACTGTGGTCCCTGAGCTG27240.2690830136902404No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT27030.267008585170602No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAG26670.263452421994079No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT25880.2556486194678202No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATA25040.24735090538926652No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCA25030.24725212307880753No Hit
GAATGGGGTCTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTT23910.23618850430740262No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC23340.23055791261124126No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAG23280.2299652187484874No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC23130.22848348409160282No Hit
CCTATGAGTTGACACAGCCATCCTCAGTGTCAGTGTCTCCGGGACAGACA22580.22305045701635934No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC22160.2189015999770825No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGG21960.21692595376790305No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCA20920.20665259348016995No Hit
CTGTTACTCTGCGGCTGACCAGAATGGGGTCTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAG20850.20596111730695715No Hit
GACTATTACTGTTACTCTGCGGCTGACCAGAATGGGGTCTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCA20740.20487451189190845No Hit
GCCATCCTCAGTGTCAGTGTCTCCGGGACAGACAGTCAGGATCACCTGCT20040.19795975015978037No Hit
GGTTGAGGATGAGGCTGACTATTACTGTTACTCTGCGGCTGACCAGAATGGGGTCTTCGGCGGAGGGACCAA19830.19588532164014197No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGG19710.1946999339146343No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCT19430.19193402922178307No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTG18940.18709369600929343No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA18920.1868961313883755No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTG18740.18511804980011398No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGG18660.18432779131644222No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTT18460.18235214510726278No Hit
GTAATAGACAGCCGTGTCCTCGGGTCTCAGGCTGTTCATTTGCAGAAACAACGTGTTCTTGGAATTGTCTCTGGA18300.1807716281399192No Hit
AGGTGACTGTGGTCCCTGAGCTGGAGCCGGAGAAGCGCTCAGGGATCCCA17530.17316539023457836No Hit
TCCTCAACCTGGGCCCCGGTGATGGTCAAGGTGACTGTGGTCCCTGAGCTGGAGCCGGAGAAGCGCTCAGG17430.17217756712998863No Hit
GCTCTGAATGTCTCACCATGGCCTGGATCCCTCTCCTGCTCCCCCTCCTCATTCTCTGCACAGTCTCTGTGGCCT16980.1677323631593349No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC16650.1644725469141888No Hit
CTCCGGGACAGACAGTCAGGATCACCTGCTCAGGAAATATACTGGCAGAA16640.16437376460372982No Hit
CAGTCACCTTGACCATCACCGGGGCCCAGGTTGAGGATGAGGCTGACTAT16570.16368228843051702No Hit
GCTCTAGTCTCCCGTGGTGGGGGTGAGGGTTGAGAACCTATGAACATTCT16350.16150907760041963No Hit
TCCTCATTCTCTGCACAGTCTCTGTGGCCTCCTATGAGTTGACACAGCCA16280.1608176014272068No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT15770.15577970359379925No Hit
GTAATAGTCAGCCTCATCCTCAACCTGGGCCCCGGTGATGGTCAAGGTGACTGTGGTCCCTGAGCTGGAGCCGGA15550.15360649276370184No Hit
GCCCTCTGGGATCCCTGAGCGCTTCTCCGGCTCCAGCTCAGGGACCACAGTCACCTTGACCATCACCGGGGCCCA15330.15143328193360445No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGC15220.15034667651855577No Hit
GTATATTTCCTGAGCAGGTGATCCTGACTGTCTGTCCCGGAGACACTGAC15170.1498527649662609No Hit
CCAATAGAGGGGCCTGGCCTGGCTTCTGCTGGAACCACCGAACATATTTT15170.1498527649662609No Hit
GCTCAGGAGGCAGAGCTCTGAATGTCTCACCATGGCCTGGATCCCTCTCC14740.1456051256165251No Hit
GTCTATTACTGTACGCGAGAGGCTAAACATTTCGGTGACTTCGATTTTGA14740.1456051256165251No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGTGAGGGTTGA14730.14550634330606613No Hit
GGATGAGGCTGACTATTACTGTTACTCTGCGGCTGACCAGAATGGGGTCTTCGGCGGAGGGACCAAGCTGACCGT14690.14511121406423022No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTT14670.14491364944331228No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAGACCCCATTCTGGTCAGCCGCAGAGTAACAGTAATAGTCAGC14440.14264165630275594No Hit
CTATTGGTGATTTATAAGGGCAGCGAGCGGCCCTCTGGGATCCCTGAGCG14340.14165383319816618No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGC14150.13977696929944572No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC14030.13859158157393808No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAGACCCCATTCTGGT13940.13770254077980731No Hit
CTCCTGCTCCCCCTCCTCATTCTCTGCACAGTCTCTGTGGCCTCCTATGAGTTGACACAGCCATCCTCAGTGTCA13850.13681349998567657No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC13620.1345415068451202No Hit
TCCCTCTCCTGCTCCCCCTCCTCATTCTCTGCACAGTCTCTGTGGCCTCC13080.1292072620803357No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC13040.12881213283849982No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCA13000.12841700359666391No Hit
CTGGGATCCCTGAGCGCTTCTCCGGCTCCAGCTCAGGGACCACAGTCACCTTGACCATCACCGGGGCCCAGGTT12970.128120656665287No Hit
CCCGTGGTGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGCCA12730.12574988121427166No Hit
CCTCATTCTCTGCACAGTCTCTGTGGCCTCCTATGAGTTGACACAGCCATCCTCAGTGTCAGTGTCTCCGGGACA12700.12545353428289474No Hit
TCTCTGCACAGTCTCTGTGGCCTCCTATGAGTTGACACAGCCATCCTCAGTGTCAGTGTCTCCGGGACAGACAGT12510.12357667038417428No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTG12320.12169980648545381No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAG12140.11992172489719231No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG12060.11913146641352053No Hit
CTCTATTGGTGATTTATAAGGGCAGCGAGCGGCCCTCTGGGATCCCTGAGCGCTTCTCCGGCTCCAGCTCAGG12000.1185387725507667No Hit
ACCCCATTCTGGTCAGCCGCAGAGTAACAGTAATAGTCAGCCTCATCCTCAACCTGGGCCCCGGTGATGGTCAAG11990.11843999024030773No Hit
GGCCTGGATCCCTCTCCTGCTCCCCCTCCTCATTCTCTGCACAGTCTCTG11920.11774851406709493No Hit
GTGTGACCTGGAGCGAAAGCGGACAGAACGTGACCGCCAGAAACTTCCCACCTAGCCAGGATGCCTCCGGGGAC11790.11646434403112828No Hit
ACCTGGGCCCCGGTGATGGTCAAGGTGACTGTGGTCCCTGAGCTGGAGCCGGAGAAGCGCTCAGGGATCCCAGAG11760.11616799709975136No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGTGAGGGTTGAG11760.11616799709975136No Hit
GCTTGGTCCCTCCGCCGAAGACCCCATTCTGGTCAGCCGCAGAGTAACAGTAATAGTCAGCCTCATCCTCAAC11730.11587165016837445No Hit
GTATCAACGCAGAGTACGGGGTAGGCTCAGGAGGCAGAGCTCTGAATGTCTCACCATGGCCTGGATCCCTCTC11720.11577286785791546No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC11560.11419235089057192No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTAT11310.11172279312909761No Hit
GGGATCCCAGAGGGCCGCTCGCTGCCCTTATAAATCACCAATAGAGGGGCCTGGCCTGGCTTCTGCTGGAACCAC11280.1114264461977207No Hit
GACCATCACCGGGGCCCAGGTTGAGGATGAGGCTGACTATTACTGTTACTCTGCGGCTGACCAGAATGGGGTCTT11270.11132766388726172No Hit
GGCCAGGCCCCTCTATTGGTGATTTATAAGGGCAGCGAGCGGCCCTCTGG11200.11063618771404893No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAA11170.110339840782672No Hit
CGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC11140.1100434938512951No Hit
AGCCAGGCCAGGCCCCTCTATTGGTGATTTATAAGGGCAGCGAGCGGCCCTCTGGGATCCCTGAGCGCTTCTCCG11110.10974714691991817No Hit
GCCTCATCCTCAACCTGGGCCCCGGTGATGGTCAAGGTGACTGTGGTCCCTGAGCTGGAGCCGGAGAAGCGCTCA11000.10866054150486947No Hit
GGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGA10900.10767271840027975No Hit
GAGTTGACACAGCCATCCTCAGTGTCAGTGTCTCCGGGACAGACAGTCAGGATCACCTGCTCAGGAAATATACTG10820.10688245991660797No Hit
GGGTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCA10810.106783677606149No Hit
GGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAGAC10750.10619098374339515No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG10450.10322751442962601No Hit
CAGTAATAGACAGCCGTGTCCTCGGGTCTCAGGCTGTTCATTTGCAGAAACAACGTGTTCTTGGAATTGTCTCTG10430.10302994980870807No Hit
GGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGA10420.10293116749824908No Hit
TTCTCTGCACAGTCTCTGTGGCCTCCTATGAGTTGACACAGCCATCCTCA10330.10204212670411833No Hit
GGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC10150.10026404511585683No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGTAT301.4124035E-4140.0206970
AATGGTA2950.056.9575670
GTGGTAT1800.054.743971
GTATAGG200.00781503350.968521
TACGGAT200.00783643750.9332737
ACCAGGT2202.2719178E-950.9166170
TGGTATC1950.050.500442
AACGCTG352.4968262E-548.507885
TAACAGT27550.048.2829970
GTGTTCA2151.08959284E-745.58812770
GGTATCA6600.043.246021
TAGGCAA1308.7012094E-443.08328670
ATTTATA21550.042.88336670
CTCGTAT2005.198581E-742.00620370
GTATCAA16500.038.509551
GGTAGAA26450.038.1152770
CAGCCGT8550.037.6663870
TATAAGA754.622234E-836.221012
GGGGGGT10450.036.17759370
AACGAAC400.002557089334.60686551
Produced by FastQC (version 0.11.5)