FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685174

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685174
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1052995
Sequences flagged as poor quality0
Sequence length18-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACACGGCCGTGTCCGCGGCAGTCACAGAACTCAACTTCAGGGAGAAGTGATTCTTAGACGTGTCTATTGA41920.39810255509285414No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAG35100.3333349161202095No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGACTTAGGACGGTGACCTTGGTCCCAG34070.32355329322551385No Hit
GATTATTACTGCTGCTCATATGCAGGTATTAGAAGTTATGTCTTCGGAACTGGGACCAAGGTCACCGTCCTAAG33430.3174753916210428No Hit
GACTTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATAACTTCTAATACCTGCATATGAGCAGCAGTAATAA32600.30959311297774444No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC32180.30560449004981033No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC27210.25840578540258974No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG26990.25631650672605283No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCC25730.24435063794225045No Hit
GTAATAATCAGCCTCGTCCTCAGCCTGGAGCCCAGAGATTGTCAGGGAGG22850.21700008072213067No Hit
GTCTCTGGTGGCTCCATCAGCAGTGGTGGTTACTACTGGACCTGGATCCGCCAGCACCCAGGGAAGGGCCTGGAG22060.20949767092911173No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACTTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATAACTTCTAA21170.20104558901039415No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT21170.20104558901039415No Hit
GTCCTAAGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC20510.19477775298078337No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC20450.1942079497053642No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCACTGGAACCAGCAGTGATGTT20340.19316331036709575No Hit
CAGTAATACACGGCCGTGTCCGCGGCAGTCACAGAACTCAACTTCAGGGAGAAGTGATTCTTAGACGTGTCTAT20130.19116899890312872No Hit
GGACACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC19640.18651560548720555No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAG19470.1849011628735179No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCT19110.18148234322100293No Hit
CTTATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGT18810.17863332684390715No Hit
CTTATAACCTTGTCTCCTGGTACCAACAGCACGCAGGCAAAGCCCCCAAA18630.17692391701764965No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC18510.17578431046681134No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACACCTGTGGTTCTTCCTCCTGCTGGTGGCAGCTCCCAGATGGGTC18420.1749296055536826No Hit
GACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCCTGCACTGGAACCAGCAGTGATGT18360.17435980227826345No Hit
GTCGATCACCATCTCCTGCACTGGAACCAGCAGTGATGTTGGGACTTATAACCTTGTCTCCTGGTACCAACAGCA18160.17246045802686621No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG18120.17208058917658678No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGG18080.17170072032630734No Hit
GTGTATTACTGTGCGAGAATGCCTCCGGGGGACCTTGATGCTTTTGATAT17920.17018124492518957No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA17720.16828190067379237No Hit
CTCCTGCACTGGAACCAGCAGTGATGTTGGGACTTATAACCTTGTCTCCTGGTACCAACAGCACGCAGGCAAAGC17040.16182413021904188No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG16610.15774054007853788No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGC14840.14093134345367261No Hit
GGCCTGGGCTCTGCTGCTCCTCACTCTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGC14520.13789239265143707No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT14390.13665781888802891No Hit
GCCTTGGGCTGACTTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATAACTTCTAATACCTGCATATGAGCAG14370.13646788446288918No Hit
GGCAAAGCCCCCAAACTCATGATTTATGAGGTCAGTAAGCGGCCCTCAGG14260.13542324512462073No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG14200.13485344184920156No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAGTTGAGGGAGATGGGGTAGGTGGAGT14190.1347584746366317No Hit
CCCCTGAGGGCCGCTTACTGACCTCATAAATCATGAGTTTGGGGGCTTTGCCTGCGTGCTGTTGGTACCAGGAGA14100.13390376972350296No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGTGACT13900.13200442547210575No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGC13580.12896547466987024No Hit
GTATTACTGTGCGAGAATGCCTCCGGGGGACCTTGATGCTTTTGATATCTGGGGCCAAGGGACAATGGTCACCG13340.1266862615681936No Hit
GCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCCTGCACTGGAACCAGCAG13310.126401359930484No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGA13040.12383724519109778No Hit
CTTCTAATACCTGCATATGAGCAGCAGTAATAATCAGCCTCGTCCTCAGC12730.1208932616014321No Hit
AGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA12530.1189939173500349No Hit
CTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTG12380.11756940916148699No Hit
GTACCAACAGCACGCAGGCAAAGCCCCCAAACTCATGATTTATGAGGTCAGTAAGCGGCCCTCAGGGGTTTCTAA12280.1166197370357884No Hit
CTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCCCG12230.11614490097293909No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC12220.11604993376036923No Hit
GGTTATAAGTCCCAACATCACTGCTGGTTCCAGTGCAGGAGATGGTGATC12150.11538516327238021No Hit
CTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCC12000.11396065508383231No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGT11910.11310595017070357No Hit
TCCTAAGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGG11900.11301098295813371No Hit
GTAATAGATGTACCCAATCCACTCCAGGCCCTTCCCTGGGTGCTGGCGGATCCAGGTCCAGTAGTAACCACCA11760.11168144198215567No Hit
CAAGAGGCAGCGCTCTCGGGACGTCTCCACCATGGCCTGGGCTCTGCTGC11650.11063680264388721No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGG11620.11035190100617762No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAGTGTCCTGCCGGGCTGTGCCGAGCCA11570.10987706494332831No Hit
TCTCTGGGCTCCAGGCTGAGGACGAGGCTGATTATTACTGCTGCTCATAT11560.10978209773075845No Hit
CTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCC11540.10959216330561873No Hit
GGGCTCTGCTGCTCCTCACTCTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCC11470.10892739281762971No Hit
GGTACCAACAGCACGCAGGCAAAGCCCCCAAACTCATGATTTATGAGGTC11420.10845255675478041No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC11400.10826262232964069No Hit
CACCTGCACTGTCTCTGGTGGCTCCATCAGCAGTGGTGGTTACTACTGGA11300.10731295020394208No Hit
CCTTGGGGCTGGTCGGGGATGCTGAAGAGACGGTGACCATTGTCCCTTGGCCCCAGATATCAAAAGCATCAAGG11290.10721798299137222No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATGGGTGGGTTTACCCGCCAAGCGG11140.10579347480282432No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC11090.10531863873997503No Hit
ACCATGGCCTGGGCTCTGCTGCTCCTCACTCTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTCTGCCCTGAC11080.10522367152740517No Hit
CTCTCGGGACGTCTCCACCATGGCCTGGGCTCTGCTGCTCCTCACTCTCCTCACTCAGGACACAGGGTCCTGGGC10940.10389413055142713No Hit
CTGCTGCTCCTCACTCTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTG10890.10341929448857781No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT10830.10284949121315866No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT10830.10284949121315866No Hit
GACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTT10790.10246962236287922No Hit
GCCTGGAGCCCAGAGATTGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCC10780.10237465515030934No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC10730.10189981908746006No Hit
CTACTGGACCTGGATCCGCCAGCACCCAGGGAAGGGCCTGGAGTGGATTGGGTACATCTATTACAGTGGGATCAC10700.10161491744975049No Hit
CCCGTGGGTGGGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCA10550.10019040926120258No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG4550.0119.73302570
TCTACGG150.002540229467.6984562
CAGTTTA300.00585351554.83054469
TAGATGA600.006001927454.47852770
AATGGTA2650.053.4506370
CGCGTAT200.007604349451.32220539
GTTATAC200.00793424750.7738383
GTCGTAT750.050.1817139
GGTATCA7700.049.258631
ATGTCGT752.0008883E-1145.4974437
CTATTGA10150.044.83679669
TATACAC5200.044.264373
TACGTGA507.9742126E-443.86443369
CGTATGC4800.043.58592241
AAAAGGG4400.043.43377767
AAAGGGG4900.043.3846668
CTTATAC5250.043.2185861
TCGTATG4600.043.1901640
GTACGGA405.5808225E-542.3316351
TTGAAAA4750.042.15447658
Produced by FastQC (version 0.11.5)