FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685175

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685175
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1052995
Sequences flagged as poor quality0
Sequence length18-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG52010.4939244725758432No Hit
GTAATACACGGCCGTGTCCGCGGCAGTCACAGAACTCAACTTCAGGGAGAAGTGATTCTTAGACGTGTCTATT40240.3821480633811177No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT33640.31946970308500994No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC31930.3032303097355638No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA31820.3021856703972953No Hit
GACTTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATAACTTCTAATACCTGCATATGAGCAGCAGTAATAAT31650.30057122778360773No Hit
GATTATTACTGCTGCTCATATGCAGGTATTAGAAGTTATGTCTTCGGAACTGGGACCAAGGTCACCGTCCTAAGT30920.2936386212660079No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC26290.24966880184616264No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGG24830.23580358881096303No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCCT22820.2167151790844211No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACTTAGGACGGTGACCTTGGTC19990.18983945792715065No Hit
GGACACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC19820.18822501531346303No Hit
GTCTCTGGTGGCTCCATCAGCAGTGGTGGTTACTACTGGACCTGGATCCG19770.1877501792506137No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCACTGGAACCAGCAGTGATGTT19460.18480619566094808No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT19220.1825269825592714No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC19140.18176724485871254No Hit
GTAATAATCAGCCTCGTCCTCAGCCTGGAGCCCAGAGATTGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCAGA18640.17701888423021953No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTA18590.1765440481673702No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACACCTGTGGTTCTTCCTCCTGCTGGTGGCAGCTCCCAGATGGGTC18260.17341013015256482No Hit
CTTATAACCTTGTCTCCTGGTACCAACAGCACGCAGGCAAAGCCCCCAAACTCATGATTTATGAGGTCAGTAAGC17590.16704732691038418No Hit
GTCCTAAGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAG17510.1662875892098253No Hit
CAGTAATACACGGCCGTGTCCGCGGCAGTCACAGAACTCAACTTCAGGGAGAAGTGATTCTTAGACGTGTCTATT17260.16391340889557882No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGT17110.1624889007070309No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGT16980.1612543269436227No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC16450.1562210646774201No Hit
GTGTATTACTGTGCGAGAATGCCTCCGGGGGACCTTGATGCTTTTGATATCTGGGGCCAAGGGACAATGGTCACC16280.15460662206373252No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT16230.15413178600088318No Hit
CTCCTGCACTGGAACCAGCAGTGATGTTGGGACTTATAACCTTGTCTCCTGGTACCAACAGCACGCAGGCAAAGC16110.15299217945004487No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG15880.15080793356093808No Hit
GACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCT15830.1503330974980888No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGTGACTTCTACCCGG15800.1500481958603792No Hit
GTCGATCACCATCTCCTGCACTGGAACCAGCAGTGATGTTGGGACTTATAACCTTGTCTCCTGGTACCAACAGCA15570.14786394997127242No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTT14570.13836722871428642No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCT14260.13542324512462073No Hit
GGCCTGGGCTCTGCTGCTCCTCACTCTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGC14140.13428363857378242No Hit
CCCCTGAGGGCCGCTTACTGACCTCATAAATCATGAGTTTGGGGGCTTTGCCTGCGTGCTGTTGGTACCAGGA14130.13418867136121254No Hit
GGCAAAGCCCCCAAACTCATGATTTATGAGGTCAGTAAGCGGCCCTCAGG13960.13257422874752492No Hit
GCCTTGGGCTGACTTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATAACTTCTAATACCTGCATATGAGCA13920.13219435989724546No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTG13690.1300101140081387No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAG13210.1254516878047854No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC13030.12374227797852791No Hit
GCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCCTGCACTGGAACCAGCAGT12900.12250770421511972No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGACTTGCCGGCTAGGCACTGTGTGGCC12530.1189939173500349No Hit
GTATTACTGTGCGAGAATGCCTCCGGGGGACCTTGATGCTTTTGATATCTGGGGCCAAGGGACAATGGTCACCGT12260.11642980261064868No Hit
AGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCT12230.11614490097293909No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTTCCATGGCTCGGCACAGCCCGGCAG12230.11614490097293909No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC12200.11585999933522952No Hit
CTTCTAATACCTGCATATGAGCAGCAGTAATAATCAGCCTCGTCCTCAGCCTGGAGCCCAGAGATTGTCAGGGAG12190.11576503212265966No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC11970.11367575344612271No Hit
CTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAG11950.113485819020983No Hit
GGTTATAAGTCCCAACATCACTGCTGGTTCCAGTGCAGGAGATGGTGATCGACTGTCCAGGAGACCCAGACACG11670.1108267370690269No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG11650.11063680264388721No Hit
CTGCTGCTCCTCACTCTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTC11470.10892739281762971No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG11460.10883242560505986No Hit
GTAATAGATGTACCCAATCCACTCCAGGCCCTTCCCTGGGTGCTGGCGGATCCAGGTCCAGTAGTAACCACCA11420.10845255675478041No Hit
AGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCCTGCACTGGAACCA11360.10788275347936124No Hit
CTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCCCGTCAAG11260.10693308135366265No Hit
TCTCTGGGCTCCAGGCTGAGGACGAGGCTGATTATTACTGCTGCTCATATGCAGGTATTAGAAGTTATGTCTTCG11170.1060783764405339No Hit
GGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGC11130.10569850759025448No Hit
GGGCTCTGCTGCTCCTCACTCTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCT11090.10531863873997503No Hit
GTACCAACAGCACGCAGGCAAAGCCCCCAAACTCATGATTTATGAGGTCA11040.10484380267712572No Hit
CTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTG10870.10322936006343808No Hit
GACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTG10750.10208975351259977No Hit
CTCTCGGGACGTCTCCACCATGGCCTGGGCTCTGCTGCTCCTCACTCTCCTCACTCAGGACACAGGGTCCTGGGC10700.10161491744975049No Hit
TCCTAAGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAG10620.10085517974919159No Hit
ACCTGAGCCTGACGCCCGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC10610.10076021253662172No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGG10530.10000047483606285No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGGTA200.00620284151.6116670
TTGTTAT800.005067358356.85437470
AATGGTA2505.638867E-1154.58019670
GCTTAGT200.0079445750.7571371
TAATCCA950.009997426547.87736570
CTGGAGT15450.047.1026570
ATTGTTT1305.8802956E-446.6497470
CCACCTT7700.045.286670
GCACCGT12250.043.31761670
TGGTATC2750.043.015522
TCCCAGT27000.042.67587370
GGTATCA8850.042.058641
GTATCAA22700.039.3535461
GGGATGT1550.001397631839.12558770
GAGCCAT9850.038.48011470
ACTCAGT9600.037.90291270
CCCTACA10850.037.72824570
GTGGTAT3300.036.914281
GACAGGT2900.035.004921
CCTGGTA22200.034.8297170
Produced by FastQC (version 0.11.5)