FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685176

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685176
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1333587
Sequences flagged as poor quality0
Sequence length18-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAAACGGCCGTGTCTGCGGCGGTCACAGAGCTCAGCCTCAGGGAGAATTGGTTCTTGGACGTGTCTACTGA48330.3624060522485597No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGG42470.3184644121455893No Hit
GTCTCTGGTGGCTCCATCAGTAGTAGTAGTGACTACTGGAGCTGGATCCGGCAGCCCGCCGAGAAGGGACTGGAG37880.2840459602560613No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCTTAGCACTCAGGCTGCTATCCCATGT34210.2565261958912317No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAG29290.21963321478088793No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG28390.21288449872411772No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCTTAGCACTCAGG27930.2094351549617685No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACATCTGTGGTTCTTCCTCCTCCTGGTGGCAGCTCCCAGATGGGTC27080.20306136757481888No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA26850.2013366956936443No Hit
GTTTATTACTGTGCGAGAGAGGTCCAGGGAGGTACCTACTACTTTGACTA24760.18566467729514458No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT23900.17921590417423086No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC23680.17756621802702036No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCT22980.17231721664953242No Hit
GTAGTAGTAGTGACTACTGGAGCTGGATCCGGCAGCCCGCCGAGAAGGGACTGGAGTGGGTTGGGCGTATCTATC22970.1722422309155683No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAGTTGAGGGAGATGGGGTAGGTGGAGT22930.17194228797971187No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG22820.1711174449061066No Hit
CCTTAGCACTCAGGCTGCTATCCCATGTTCCGCAGTAATAATCGGCCTCGTCCCCAGTCTGGAGTCCGGTGATGC21810.16354388577573117No Hit
GTAATAATCGGCCTCGTCCCCAGTCTGGAGTCCGGTGATGCCCAGGGTGG21520.1613692994907719No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT21310.1597945990775255No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC21120.15836987013220735No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC20690.15514548357175048No Hit
GTGCTAAGGTATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGT20470.15349579742453998No Hit
CACCTGCACTGTCTCTGGTGGCTCCATCAGTAGTAGTAGTGACTACTGGA19990.14989648219426255No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGG19590.146897052835698No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC18990.14239790879785122No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG18540.13902355076946613No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG18510.13879859356757376No Hit
GGACAGAAGGTCACCATCTCCTGCTCTGGAAGCAGCTCCAACATTGGGAATAATTATGTATCCTGGTACCAGCAG18290.1371489074203633No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG18240.13677397875054273No Hit
GGACTGGAGTGGGTTGGGCGTATCTATCCCAGTGGGAGCATCAACTACAA17990.13489933540143986No Hit
GATTATTACTGCGGAACATGGGATAGCAGCCTGAGTGCTAAGGTATTCGG17910.13429944952972697No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC17910.13429944952972697No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC17560.131674948840983No Hit
GGAGTGGGTTGGGCGTATCTATCCCAGTGGGAGCATCAACTACAACCCCT17320.1298752912258443No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGC16870.12650093319745917No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC16860.12642594746349506No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT16550.12410138971060757No Hit
GGGTTCCCTGGCCCCAGTAGTCAAAGTAGTAGGTACCTCCCTGGACCTCTCTCGCACAGTAATAAACGGCCGTG16180.12132691755393536No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAGGTCCCCGGAGGCATCCTGGCTGGG16160.12117694608600714No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA16080.12057706021429423No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGG16050.12035210301240189No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC15890.11915233126897606No Hit
GTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGG15890.11915233126897606No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG15850.11885238833311963No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA15690.11765261658969381No Hit
CCTTGGGGCTGGTCGGGGATGCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAGTAGTAGGTAC15540.1165278305802321No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA15520.11637785911230389No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC15470.11600293044248332No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAGTGTCCTGCCGGGCTGTGCCGAGCCA15310.1148031586990575No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG15120.11337842975373935No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATGGGTGGGTTTACCCGCCAAGCGG15100.11322845828581113No Hit
GTACGGGACTTTCTGAGAGTCCTGGACCTCCTGTGCAAGAACATGAAACATCTGTGGTTCTTCCTCCTCCTGG15020.11262857241409821No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC14960.11217865801031353No Hit
AGGCAGGACTCAGGACAATCTTCATCATGACCTGCTCCCCTCTCCTCCTCACCCTTCTCATTCACTGCACAGGG14910.11180372934049299No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC14790.11090390053292362No Hit
GTCTGGAGTCCGGTGATGCCCAGGGTGGCTGACGTGCCAGACTTGGAGCC14450.10835438557814377No Hit
GTTTGGGGGCTGTTCCTGGGAACTGCTGGTACCAGGATACATAATTATTC14440.10827939984417966No Hit
GGGCATCACCGGACTCCAGACTGGGGACGAGGCCGATTATTACTGCGGAA14430.10820441411021553No Hit
GGACAATCTTCATCATGACCTGCTCCCCTCTCCTCCTCACCCTTCTCATTCACTGCACAGGGTCCTGGGCCCAG14370.10775449970643085No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCC14280.10707962810075383No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCTTAGCACTCAGGC14260.1069296566328256No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCTGACGCCTGAGCAG14190.1064047564950768No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT13970.10475507034786631No Hit
GTCACGGACTTGCCGGCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCT13710.10280544126479937No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA13650.10235552686101469No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT13570.10175564098930179No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT13570.10175564098930179No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG3550.0107.370770
CGCGTAT408.711977E-751.35769339
CGTATGC2400.047.20139741
AGTATCG752.0008883E-1145.4324734
GGTATCA9700.043.992551
ACCGATC3850.043.1491327
TGGTATC2200.043.087652
TCTCGTA2550.041.57750338
CTTATAC4000.041.487421
TATCTCG2800.041.45579536
TCGTATG2400.041.42803240
TTATACA4050.040.9598662
TATACAC4100.040.4603583
CAGTATC4050.040.3509133
ACGAGAC4050.040.1329822
ATCTCGT2750.039.7551837
TCAGTAT4200.039.6785332
CCCACGA4300.039.37022419
ATGCCGT2800.039.3700544
CACGAGA4150.039.16444821
Produced by FastQC (version 0.11.5)