FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004685177

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685177
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1333587
Sequences flagged as poor quality0
Sequence length18-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAAACGGCCGTGTCTGCGGCGGTCACAGAGCTCAGCCTCAGGGAGAATTGGTTCTTGGACGTGTCTACT45610.34200993261032087No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG43780.32828754329488813No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGT39780.2982932497092428No Hit
GTCTCTGGTGGCTCCATCAGTAGTAGTAGTGACTACTGGAGCTGGATCCG37760.28314613144849193No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT28500.21370934179772297No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG28290.21213464138447657No Hit
GTCCTGGGCCCAGTCTGTGTTGACGCAGCCGCCCTCAGTGTCTGCGGCCCCAGGACAGAAGGTCACCATCTCCT27580.20681065427302453No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACATCTGTGGTTCTTCCTCCTCCTGGTGGCAGCTCCCAGATGGGTC27490.20613578266734756No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCTTAGCACTCAGGCTGCTATCCCATGTT26620.19961202381246967No Hit
GTGTTGACGCAGCCGCCCTCAGTGTCTGCGGCCCCAGGACAGAAGGTCAC23120.17336701692503No Hit
GTGCTAAGGTATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCC22760.17066753050232192No Hit
GTTTATTACTGTGCGAGAGAGGTCCAGGGAGGTACCTACTACTTTGACTACTGGGGCCAGGGAACCCTGGTCACC22300.1672181867399727No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAG22070.1654935148587981No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAG21760.1631689571059106No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGACTTGCCGGCTAGGCACTGTGTGGCC21670.16249408550023356No Hit
GTAGTAGTAGTGACTACTGGAGCTGGATCCGGCAGCCCGCCGAGAAGGGACTGGAGTGGGTTGGGCGTATCTATC21220.1591197274718485No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT21050.15784496999445854No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCTTAGCACTCAGG20490.1536457688924682No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC20440.15327084022264764No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT19720.1478718673772315No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCA19670.1474969387074109No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAG19400.14547232389037987No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC19200.14397260921109759No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG18980.14232292306388708No Hit
GGACAGAAGGTCACCATCTCCTGCTCTGGAAGCAGCTCCAACATTGGGAA18570.13924850797135843No Hit
CACCTGCACTGTCTCTGGTGGCTCCATCAGTAGTAGTAGTGACTACTGGAGCTGGATCCGGCAGCCCGCCGAGAA18570.13924850797135843No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC18430.13819870769586087No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATA18330.13744885035621973No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC18320.13737386462225562No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG17620.1321248632447677No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAACGTGGTCATCGCCTGCCTGGTCCAGG16890.12665090466538742No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGG16820.1261260045276386No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG16660.12492623278421282No Hit
CCTTAGCACTCAGGCTGCTATCCCATGTTCCGCAGTAATAATCGGCCTCGTCCCCAGTCTGGAGTCCGGTGATGC16530.12395141824267933No Hit
GGGTTCCCTGGCCCCAGTAGTCAAAGTAGTAGGTACCTCCCTGGACCTCTCTCGCACAGTAATAAACGGCCGTGT16350.12260167503132528No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCA16160.12117694608600714No Hit
GGAGTGGGTTGGGCGTATCTATCCCAGTGGGAGCATCAACTACAACCCCT16050.12035210301240189No Hit
GATTATTACTGCGGAACATGGGATAGCAGCCTGAGTGCTAAGGTATTCGGCGGAGGGACCAAGCTGACCGTCCTA16040.12027711727843778No Hit
GGACTGGAGTGGGTTGGGCGTATCTATCCCAGTGGGAGCATCAACTACAA15800.11847745966329906No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC15790.11840247392933495No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTTCCATGGCTCGGCACAGCCCGGCAG15700.11772760232365792No Hit
AGGCAGGACTCAGGACAATCTTCATCATGACCTGCTCCCCTCTCCTCCTCACCCTTCTCATTCACTGCACAGGGT15680.1175776308557297No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC15450.1158529589745551No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA15190.11390332989148813No Hit
GTAATAATCGGCCTCGTCCCCAGTCTGGAGTCCGGTGATGCCCAGGGTGGCTGACGTGCCAGACTTGGAGCCAGA15170.11375335842355992No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTG14930.1119537008084212No Hit
GTACGGGACTTTCTGAGAGTCCTGGACCTCCTGTGCAAGAACATGAAACATCTGTGGTTCTTCCTCCTCCTGGTG14790.11090390053292362No Hit
CCTTGGGGCTGGTCGGGGATGCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAGTAGTAGGTAC14700.11022902892724659No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTT14660.10992908599139013No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGCAGAG14460.10842937131210788No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCT14450.10835438557814377No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTG14340.10752954250453851No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA14320.10737957103661028No Hit
GGACAATCTTCATCATGACCTGCTCCCCTCTCCTCCTCACCCTTCTCATTCACTGCACAGGGTCCTGGGCCCAGT14210.10655472796300505No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGCAGAGGCTCAGCGGGAAGACCTTGGG14190.1064047564950768No Hit
GGGCATCACCGGACTCCAGACTGGGGACGAGGCCGATTATTACTGCGGAACATGGGATAGCAGCCTGAGTGCTAA14010.10505501328372277No Hit
GGACTGGTGAAGCCTTCACAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCAGTAGTAGTAGT13770.10325535566858406No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC13740.10303039846669172No Hit
CCCTCAGTGTCTGCGGCCCCAGGACAGAAGGTCACCATCTCCTGCTCTGG13500.10123074085155298No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)