FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685178

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685178
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1001089
Sequences flagged as poor quality0
Sequence length18-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAATGCCTGGCCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCA73340.7326021962083291No Hit
GCATTATACTGCTGACAGTAATAAACTGCAAAATCTTCAGACTGCAGGCTGCTGATGGTGAAGGTGAACTCTGTC50370.503152067398603No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTAT48090.48037686958901754No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC44560.4451152694715455No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCT31680.3164553800910808No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG29260.29228170522301217No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC28280.28249236581362897No Hit
CATCTATGGTGCATCCACCAGGGCCACTGGCATTCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGAGTTCAC27350.2732024824965612No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC27300.27270302640424576No Hit
GTCTGGGACAGAGTTCACCTTCACCATCAGCAGCCTGCAGTCTGAAGATTTTGCAGTTTATTACTGTCAGCAGTA25620.25592130170244604No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG25320.25292456514855327No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCC24910.24882902519156638No Hit
GAGTTCACCTTCACCATCAGCAGCCTGCAGTCTGAAGATTTTGCAGTTTATTACTGTCAGCAGTATAATGCC24760.24733065691462No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG24730.2470309832592307No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG24610.24583228863767356No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCTGGAATGCCAGTGGCCCTGGTGGATGCACCATAGATGAGGAGCC24540.24513305010843192No Hit
GTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTCGTGCAGGGCCAGTCAGAGTGTTACTAGCAACTTAGCCTGGTA23820.23794088237908917No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA23760.2373415350683106No Hit
GCTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA23350.23324599511132374No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT23280.2325467565820821No Hit
CCATAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGCTGGTACCAGGCTAAGTTGCTAGTAACACTCTGAC23270.232446865363619No Hit
GAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCACTGGAGAAATAGTGA22840.228151542969706No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAAC22410.22385622057579296No Hit
GTCTCCAGCCACCCTGTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTCGTGCAGGGCCAGTCAGAGTGTTACTAG21250.21226883923407408No Hit
CTATTATACTGGGGGCACCTACTACAACCCGTCCCTCAGGAGCCGAATCACCATATCCGTAGACACGTCCAAGAA20950.20927210268018132No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT20550.20527645394165756No Hit
GGAGAAATAGTGATGACGCAGTCTCCAGCCACCCTGTCTGTGTCTCCAGG20530.20507667150473133No Hit
GAGTGTTACTAGCAACTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGGTGCATC20450.2042775417570266No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACT20320.20297895591700638No Hit
GTTTATTACTGTCAGCAGTATAATGCCTGGCCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAA18970.18949364142448874No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCC17880.17860549861201153No Hit
GTAATAAACTGCAAAATCTTCAGACTGCAGGCTGCTGATGGTGAAGGTGAACTCTGTCCCAGACCCACTGCCA17250.17231235184883661No Hit
GGGCCAGTCAGAGTGTTACTAGCAACTTAGCCTGGTACCAGCAGAAACCT17100.1708139835718902No Hit
GCTCAGCTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGA16980.1696152889503331No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTG16530.16512018411949386No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC16390.16372170706101055No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA16200.1618237739102118No Hit
GTCTCTGGTGGCTCCATCAGCAGTAGTCGTTTCTACTGGGGCTGGATCCGCCAGCCCCCAGGGAAGGGGCTGGAG16040.1602255144148023No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT16040.1602255144148023No Hit
GTCAGAGTGTTACTAGCAACTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGG15660.1564296481132047No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC15600.15583030080242616No Hit
GTATAATAGATACTCCCAATCCACTCCAGCCCCTTCCCTGGGGGCTGGCGGATCCAGCCCCAGTAGAAACGACTA15320.15303334668545954No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG15250.15233410815621787No Hit
GGTGAACTCTGTCCCAGACCCACTGCCACTGAACCTGGCTGGAATGCCAGTGGCCCTGGTGGATGCACCATAGA15010.14993671891310362No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCA14750.1473395472330632No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT14700.14684009114074772No Hit
CAGTAATACACAGCCGAATCCGCGGCGGTCACTGAGCTCAGCTTCAAGAA14320.14304422483915016No Hit
GGCATTATACTGCTGACAGTAATAAACTGCAAAATCTTCAGACTGCAGGCTGCTGATGGTGAAGGTGAAC14270.1425447687468347No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC14090.140746726814499No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC14010.13994759706679427No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA13760.13745031660521692No Hit
TCTATGGTGCATCCACCAGGGCCACTGGCATTCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGAGTTCACCT13750.13735042538675382No Hit
CTGCTACTCTGGCTCCCAGATACCACTGGAGAAATAGTGATGACGCAGTC13270.13255564690052535No Hit
GTATTACTGTGGTTGGGTAGTGGGACGTACTACGTGGAACTACTTTGACGACTGGGGCCAGGGAACCCTGGTCAC12980.1296588015650956No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG12960.12945901912816943No Hit
CACCAGGGCCACTGGCATTCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGAGTTCACCTTCACCATCAGCAG12880.1286598893804647No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTT12590.12576304404503497No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA12350.12336565480192072No Hit
GCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGGTGCATCCACCAGGGCCACTGGCATTCCAGCCAGGT12290.12276630749114215No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGG12110.12096826555880646No Hit
GTGTATTACTGTGGTTGGGTAGTGGGACGTACTACGTGGAACTACTTTGA12090.12076848312188027No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA12080.12066859190341718No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC12030.12016913581110171No Hit
AGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCT11920.11907033240800768No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT11870.11857087631569221No Hit
GACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCT11750.11737218169413509No Hit
CCCTGTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTCGTGCAGGGCCAGTCAGAGTGTTACTAGCAACTTAGC11750.11737218169413509No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT11720.1170725080387458No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA11690.11677283438335652No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA11530.11517457488794702No Hit
GTAATACACAGCCGAATCCGCGGCGGTCACTGAGCTCAGCTTCAAGAAGGACTGATTCTTGGACGTGTCTACGGA11470.11457522757716847No Hit
CAGTAATAAACTGCAAAATCTTCAGACTGCAGGCTGCTGATGGTGAAGGTGAACTCTGTCCCAGACCCACTGCCA11420.114075771484853No Hit
GGTTTCTGCTGGTACCAGGCTAAGTTGCTAGTAACACTCTGACTGGCCCTGCACGAGAGGGTGGCTCTTTCCC11280.11267729442636969No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG11280.11267729442636969No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC11260.1124775119894435No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT11140.11127881736788638No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC11080.1106794700571078No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTT11050.11037979640171852No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTAGGTGGGAAGTTTCTGGCGGT11050.11037979640171852No Hit
CTCTCGTGCAGGGCCAGTCAGAGTGTTACTAGCAACTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGG11040.11027990518325544No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC11000.10988034030940305No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG10950.1093808842170876No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTG10900.10888142812477213No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA10820.10808229837706738No Hit
CCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCACTGGAGAAATAGTGATGACGCAGTCTC10780.107682733503215No Hit
GGGGAAAGAGCCACCCTCTCGTGCAGGGCCAGTCAGAGTGTTACTAGCAACTTAGCCTGGTACCAGCAGAAAC10670.10658393010012097No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA10630.10618436522626859No Hit
GTGCAGGGCCAGTCAGAGTGTTACTAGCAACTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCTCC10540.10528534426010076No Hit
CCTCATCTATGGTGCATCCACCAGGGCCACTGGCATTCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGAGT10470.1045861057308591No Hit
GCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCACTGGAGAAATAGTGATGACGCAGTCTCCAGCCACC10450.1043863232939329No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT10440.10428643207546981No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC10410.10398675842008052No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGT10400.10388686720161744No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCT10310.1029878462354496No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA10300.10288795501698651No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGG10290.1027880637985234No Hit
GTATCAACGCAGAGTACGGGGAGGAACTGCTCAGCTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCT10210.10198893405081864No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC10200.10188904283235556No Hit
GAACTGCTCAGCTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTC10180.10168926039542939No Hit
CTCCCAGGCTCCTCATCTATGGTGCATCCACCAGGGCCACTGGCATTCCAGCCAGGTTCAGTGGCAGTGGGTC10110.10099002186618772No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGAACG200.007615975651.30219737
TGTTAGA10650.050.67209270
GGTATCA8050.048.049631
AATGGTA2600.047.89861370
TCTACGG8200.043.0308770
TCTTATA950.042.8501052
GTATCAA21900.039.042491
TTTCGAG350.00146421838.76914212
GAGCAAA16800.038.29988570
GTCGAGG7450.037.61166470
GCTTGTA1100.037.014311
TCGTATG1300.036.92905440
AAAGGGG2301.6370905E-1136.09750770
ACGACTA5350.035.72661669
CGTATGC1300.034.32766341
TACTATC400.002732009234.14300535
AGGTATA502.0551246E-433.9233
ATCAACG25500.033.2578433
TATCAAC25650.033.195612
AATCTCG1550.033.0682236
Produced by FastQC (version 0.11.5)