FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004685179

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685179
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1001089
Sequences flagged as poor quality0
Sequence length18-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAATGCCTGGCCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT67960.6788607206751847No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT49000.48946697046915905No Hit
GCATTATACTGCTGACAGTAATAAACTGCAAAATCTTCAGACTGCAGGCTGCTGATGGTGAAGGTGAACTCTGTC47950.47897839253053426No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTA42950.4290327832989874No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTT29700.29667691883538827No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC29510.2947789856845895No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGC27160.2713045493457624No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTC26010.2598170592225067No Hit
GTCTGGGACAGAGTTCACCTTCACCATCAGCAGCCTGCAGTCTGAAGATT24580.2455326149822843No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT24130.24103751015144506No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCC23660.2363426228836797No Hit
CATCTATGGTGCATCCACCAGGGCCACTGGCATTCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGAGTTCAC23560.23534371069904875No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG23510.23484425460673325No Hit
GAGTTCACCTTCACCATCAGCAGCCTGCAGTCTGAAGATTTTGCAGTTTATTACTGTCAGCAGTATAATGCCTG23200.23174762683437736No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGTTA23180.23154784439745119No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG22890.22865099906202146No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCTGGAATGCCAGTGGCCCTGGTGGATGCACCATAGATGAGGAGCCTG22690.22665317469275958No Hit
GAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCACTGGAGAAATAGT21940.21916133330802753No Hit
GTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTCGTGCAGGGCCAGTCAGAGTGTTACTAGCAACTTAGCCTGGTA21920.21896155087110136No Hit
CCATAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGCTGGTACCAGGCTAAGTTGCTAGTAACACTCTGACTG21530.21506579335104073No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAA21450.214266663603336No Hit
CTATTATACTGGGGGCACCTACTACAACCCGTCCCTCAGGAGCCGAATCA20560.20537634516012063No Hit
GCTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA20030.20008211058157666No Hit
GTCTCCAGCCACCCTGTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTCGTGCAGGGCCAGTCAGAGTGTTACTA19690.1966858091538315No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT19640.19618635306151602No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACT19160.19139157457528752No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGA18950.18929385898756254No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT18940.18919396776909944No Hit
GAGTGTTACTAGCAACTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGGTGCATC18460.18439918928287094No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT18360.18340027709824003No Hit
GGAGAAATAGTGATGACGCAGTCTCCAGCCACCCTGTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTCGTGCAGG18200.18180201760283052No Hit
GTTTATTACTGTCAGCAGTATAATGCCTGGCCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAA17400.17381072012578302No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCC16670.16651866117797717No Hit
GTAATAAACTGCAAAATCTTCAGACTGCAGGCTGCTGATGGTGAAGGTGAACTCTGTCCCAGACCCACTGCCA16390.16372170706101055No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTG16230.16212344756560104No Hit
GCTCAGCTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCA15780.15762834273476184No Hit
GGGCCAGTCAGAGTGTTACTAGCAACTTAGCCTGGTACCAGCAGAAACCT15610.15593019202088926No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC15450.15433193252547975No Hit
GTCTCTGGTGGCTCCATCAGCAGTAGTCGTTTCTACTGGGGCTGGATCCG15410.15393236765162738No Hit
GGTGAACTCTGTCCCAGACCCACTGCCACTGAACCTGGCTGGAATGCCAGTGGCCCTGGTGGATGCACCATAGAT15290.15273367303007024No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT15010.14993671891310362No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACA14890.1487380242915465No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA14850.14833845941769414No Hit
GTATAATAGATACTCCCAATCCACTCCAGCCCCTTCCCTGGGGGCTGGCGGATCCAGCCCCAGTAGAAACGACTA14310.1429443336206871No Hit
GGCATTATACTGCTGACAGTAATAAACTGCAAAATCTTCAGACTGCAGGCTGCTGATGGTGAAGGTGAACT14180.14164574778066685No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT13920.13904857610062643No Hit
CTGCTACTCTGGCTCCCAGATACCACTGGAGAAATAGTGATGACGCAGTCTCCAGCCACCCTGTCTGTGTCTCCA13630.1361517307651967No Hit
GTCAGAGTGTTACTAGCAACTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGGT13330.1331549942113039No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCT13320.1330551029928408No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG12960.12945901912816943No Hit
CACCAGGGCCACTGGCATTCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGAGTTCACCTTCACCATCAGCA12950.12935912790970633No Hit
TCTATGGTGCATCCACCAGGGCCACTGGCATTCCAGCCAGGTTCAGTGGC12910.12895956303585396No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGG12870.1285599981620016No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA12830.12816043328814922No Hit
CAGTAATACACAGCCGAATCCGCGGCGGTCACTGAGCTCAGCTTCAAGAAGGACTGATTCTTGGACGTGTCTACG12690.12676195622966588No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA12540.1252635879527195No Hit
GTATTACTGTGGTTGGGTAGTGGGACGTACTACGTGGAACTACTTTGACGACTGGGGCCAGGGAACCCTGGTCAC12330.12316587236499452No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA12300.12286619870960525No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC12260.12246663383575287No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCA12060.120468809466491No Hit
GTGTATTACTGTGGTTGGGTAGTGGGACGTACTACGTGGAACTACTTTGA11960.11946989728186005No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTA11890.11877065875261839No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAA11710.11697261682028272No Hit
CCCTGTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTCGTGCAGGGCCAGTCAGAGTGTTACTAGCAACTTAGC11530.11517457488794702No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTAC11460.11447533635870538No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA11280.11267729442636969No Hit
GCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGGTGCATCCACCAGGGCCACTGGCATTCCAGCCAGGTT11270.11257740320790659No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT11140.11127881736788638No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA10910.10898131934323523No Hit
GTAATACACAGCCGAATCCGCGGCGGTCACTGAGCTCAGCTTCAAGAAGGACTGATTCTTGGACGTGTCTACG10880.10868164568784593No Hit
CAGTAATAAACTGCAAAATCTTCAGACTGCAGGCTGCTGATGGTGAAGGTGAACTCTGTCCCAGACCCACTGCCA10850.10838197203245664No Hit
GGTTTCTGCTGGTACCAGGCTAAGTTGCTAGTAACACTCTGACTGGCCCTGCACGAGAGGGTGGCTCTTTCCC10670.10658393010012097No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT10640.10628425644473169No Hit
GACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCACTGGA10560.10548512669702693No Hit
CCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCACTGGAGAAATAGTGATGACGCAGTCTC10530.10518545304163765No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC10510.10498567060471146No Hit
CCTCATCTATGGTGCATCCACCAGGGCCACTGGCATTCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGAGTT10430.10418654085700672No Hit
CTCCCAGGCTCCTCATCTATGGTGCATCCACCAGGGCCACTGGCATTCCAGCCAGGTTCAGTGGCAGTGGGTCTG10430.10418654085700672No Hit
GCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCACTGGAGAAATAGTGATGACGCAGTCTCCAGCCACC10400.10388686720161744No Hit
GTGTGACCTGGAGCGAAAGCGGACAGAACGTGACCGCCAGAAACTTCCCACCTAGCCAGGATGCCTCCGGGGAC10390.10378697598315434No Hit
GAACTGCTCAGCTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTC10290.1027880637985234No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTAGGTGGGAAGTTTCTGGCGGTCACGTTCTGTCCGCTTTCGCTCCAG10290.1027880637985234No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT10260.10248839014313413No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGACTTGCCGTCTGGGCACTGTGTGGCC10230.10218871648774484No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC10230.10218871648774484No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)