FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004685180

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685180
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1073033
Sequences flagged as poor quality0
Sequence length18-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC35400.32990597679661293No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTAT34620.32263686205363673No Hit
CAGTAATACACGGCCGTGTCTTCGGCTCTCAGGCTGTTCATTTGCAGATGCAGTGAGTTCCTGGCGTTGTCCC32870.3063279507713183No Hit
GTAATACACGGCCGTGTCTTCGGCTCTCAGGCTGTTCATTTGCAGATGCA32380.30176145561226914No Hit
ATATAATGGTTATTCGGTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCA25790.2403467554119957No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCT25300.23578026025294654No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC24540.22869753306748253No Hit
CCATTATATTGTTGGCAATAATAAGTTGCAAAATCATCAGGCTGCAGGCTACTGATGGTGAGAGTGAATTCTGTC23300.2171415045017255No Hit
ACTAAATACTACGCAGACTCTGTGAAGGGTCGATTCACCATCTCCAGGGACAACGCCAGGAACTCACTGCATC22230.2071697701748222No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC21540.20073939944065095No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC21280.1983163611929922No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA21100.1966388731753823No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG20990.1956137416090651No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG20510.1911404402287721No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG20400.19011530866245493No Hit
GTATTACTGTGCGCGAGATCGGGGAGGTGGATACAGTTATGGTCTCTCCT20300.18918337087489387No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG19960.1860147823971863No Hit
AGCCTGGAGGGTCCCTGAGTCTCTCCTGTGAAGCCTCTGGATTCACCTTC19370.18051634945057607No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT18920.17632262940655133No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCC18850.1756702729552586No Hit
GTGTATTACTGTGCGCGAGATCGGGGAGGTGGATACAGTTATGGTCTCTCCTTTGACTACTGGGGCCAGGGAACC18320.17073100268118502No Hit
GAATTCACTCTCACCATCAGTAGCCTGCAGCCTGATGATTTTGCAACTTATTATTGCCAACAATATAATGGTTAT18170.16933309599984342No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACT17720.16513937595581868No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA17440.16252995015064775No Hit
GCACTGAACACAGAGGACTCACCATGGAGTTTGGGCTGAGCTGGGTTTTC16840.1569383234252814No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAGTTGAGGGAGATGGGGTAGGTGGAGT16730.15591319185896427No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT16610.15479486651389102No Hit
GTATTTAGTAGTACCACTACCACTAATGTATGAAAGCCACTCCAGTCCCTTCCCTGGAGCCTGGCGGATCCAGG16260.15153308425742731No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAAC15670.14603465131081708No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT15370.14323883794813394No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG15140.14109538103674352No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCC14630.13634249832018214No Hit
CCTTCCACCCTGTCTGCGTCTGTAGGAGACAGAGTCACCATCACTTGCCGGACCAGTCAGACTATTAATAATTGG14170.1320555844974013No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC14080.13121684048859633No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13950.13000532136476697No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGG13790.1285142209046693No Hit
GTACGGGAACTCTGGGAGAAGAGCCCCAGCCCCAGAATTCCCAGGAGTTT13770.12832783334715708No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG13530.12609118265701055No Hit
ACTTTAGAAAGAGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACTCTCACCATCAGTAGC13470.1255320199844739No Hit
TCAGTAGCCTGCAGCCTGATGATTTTGCAACTTATTATTGCCAACAATAT13100.12208385017049803No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTG12990.12105871860418087No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC12590.11733096745393665No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA12410.11565347943632676No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGACCAGTCAGACTATTAATAATTGGTTGGCCTGGTATCAGCAGAAA12330.1149079292062779No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC12190.11360321630369244No Hit
ATTAATAATTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAACCTCCTGATCTATAAGGCGTCTACT12140.11313724740991189No Hit
GTCTGCGTCTGTAGGAGACAGAGTCACCATCACTTGCCGGACCAGTCAGACTATTAATAATTGGTTGGCCTGGTA11950.11136656561354591No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT11950.11136656561354591No Hit
GGTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGCCAACCAATTATTAATAGTCTGACTGGTCCGGCAAGTGA11940.1112733718347898No Hit
CCTTATAGATCAGGAGGTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGCCAACCAATTATTAATAGTCTGA11910.11099379049852148No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG11900.11090059671976538No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACT11840.11034143404722874No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT11840.11034143404722874No Hit
CTCCAGGGACAACGCCAGGAACTCACTGCATCTGCAAATGAACAGCCTGAGAGCCGAAGACACGGCCGTGTATTA11730.10931630248091158No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC11570.1078252020208139No Hit
CAATATAATGGTTATTCGGTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCA11490.10707965179076505No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC11430.10652048911822841No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG11270.10502938865813075No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC11250.10484300110061853No Hit
GTTATGGTCTCTCCTTTGACTACTGGGGCCAGGGAACCCCGGTCACCGTC11210.1044702259855941No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC11060.10307231930425252No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATGGGTGGGTTTACCCGCCAAGCGG11050.10297912552549643No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATG11050.10297912552549643No Hit
GTCTGGGGGAGGCTTGGTCAAGCCTGGAGGGTCCCTGAGTCTCTCCTGTG11000.10251315663171588No Hit
GATCAGCACTGAACACAGAGGACTCACCATGGAGTTTGGGCTGAGCTGGGTTTTCCTTGTTGCTATTATAAAAGG10880.10139483128664263No Hit
TCTATAAGGCGTCTACTTTAGAAAGAGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCAC10830.10092886239286211No Hit
GGTATCAGCAGAAACCAGGGAAAGCCCCTAACCTCCTGATCTATAAGGCGTCTACTTTAGAAAGAGGGGTCCCA10800.10064928105659378No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)