FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004685181

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685181
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1073033
Sequences flagged as poor quality0
Sequence length18-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT34710.3234756060624417No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTA31900.2972881542319761No Hit
GTAATACACGGCCGTGTCTTCGGCTCTCAGGCTGTTCATTTGCAGATGCAGTGAGTTCCTGGCGTTGTCCCTGGA31170.2904850083827804No Hit
CAGTAATACACGGCCGTGTCTTCGGCTCTCAGGCTGTTCATTTGCAGATGCAGTGAGTTCCTGGCGTTGTCCCTG30530.28452060654238964No Hit
ATATAATGGTTATTCGGTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT25950.24183785587209342No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC24980.2327980593327512No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTT24580.22907030818250695No Hit
CCATTATATTGTTGGCAATAATAAGTTGCAAAATCATCAGGCTGCAGGCTACTGATGGTGAGAGTGAATTCTGTC24020.22385145657216504No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG22350.20828809551989547No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGC21950.20456034436965126No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGTTA21510.20045981810438265No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG20320.18936975843240608No Hit
ACTAAATACTACGCAGACTCTGTGAAGGGTCGATTCACCATCTCCAGGGACAACGCCAGGAACTCACTGCATCT20300.18918337087489387No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT18820.17539069161899026No Hit
GAATTCACTCTCACCATCAGTAGCCTGCAGCCTGATGATTTTGCAACTTA18450.17194252180501438No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTC18260.17017184000864838No Hit
GTGTATTACTGTGCGCGAGATCGGGGAGGTGGATACAGTTATGGTCTCTCCTTTGACTACTGGGGCCAGGGAACC17990.16765560798223356No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCC17450.16262314392940386No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT17380.1619707874781111No Hit
GTATTACTGTGCGCGAGATCGGGGAGGTGGATACAGTTATGGTCTCTCCTTTGACTACTGGGGCCAGGGAACCCC17230.16057288079676954No Hit
AGCCTGGAGGGTCCCTGAGTCTCTCCTGTGAAGCCTCTGGATTCACCTTCAGTGAGTCGTACATGACCTGGATCC17090.15926816789418405No Hit
GTATTTAGTAGTACCACTACCACTAATGTATGAAAGCCACTCCAGTCCCTTCCCTGGAGCCTGGCGGATCCAGGT16710.15572680430145205No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT16510.15386292872632995No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC16100.15004198379732964No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAA15940.14855088333723196No Hit
GCACTGAACACAGAGGACTCACCATGGAGTTTGGGCTGAGCTGGGTTTTC15570.14510271352325604No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACA15560.14500951974449994No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGA15110.1408157997004752No Hit
GAGTGAATTCTGTCCCAGATCCACTGCCGCTGAACCTTGATGGGACCCCTCTTTCTAAAGTAGACGCCTTATA14670.13671527343520656No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC14410.13429223518754782No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT14270.13298752228496233No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC13960.13009851514352308No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAG13780.12842102712591316No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCC13600.12674353910830327No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACT13460.1254388262057178No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTG13290.12385453196686402No Hit
GTACGGGAACTCTGGGAGAAGAGCCCCAGCCCCAGAATTCCCAGGAGTTT13230.12329536929432738No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGACTTGCCGGCTAGGCACTGTGTGGCC12930.12049955593164423No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT12850.11975400570159538No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG12810.11938123058657095No Hit
ACTTTAGAAAGAGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACTCTCACCATCAGTAGC12620.11761054879020497No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG12280.11444196031249738No Hit
ATTAATAATTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAA12260.11425557275498517No Hit
CCTTCCACCCTGTCTGCGTCTGTAGGAGACAGAGTCACCATCACTTGCCG12210.11378960386120465No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA12100.11276447229488747No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG11980.11164614694981422No Hit
CCTTATAGATCAGGAGGTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGCCAACCAATTATTAATAGTCT11940.1112733718347898No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG11430.10652048911822841No Hit
CAATATAATGGTTATTCGGTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT11400.1062409077819601No Hit
TCAGTAGCCTGCAGCCTGATGATTTTGCAACTTATTATTGCCAACAATATAATGGTTATTCGGTCACTTTCGGCG10970.10223357529544758No Hit
GTATCAACGCAGAGTACGGGAACTCTGGGAGAAGAGCCCCAGCCCCAGAATTCCCAGGAGTTTCCATTCGGT10930.10186080018042315No Hit
CCTTGGGGCTGGTCGGGGATGCTGAGGAGACGGTGACCGGGGTTCCCTGG10820.10083566861410599No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)