FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004685184

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685184
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1133564
Sequences flagged as poor quality0
Sequence length18-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTATACACAGCCGTGTCCTCGGCTCTCAGGTTGTTCATTTGCAGATACA60080.5300097744811938No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGG37140.32763919813967274No Hit
CTTTACAGGTTCTGTGGCCTCCTATGAGCTGACTCAGCCACTCTCAGTGT33930.2993214322261469No Hit
CCTATGAGCTGACTCAGCCACTCTCAGTGTCAGTGGCCCTGGGACAGACG33530.295792738654368No Hit
GGCCTGGACCGCTCTCCTTCTGAGCCTCCTTGCTCACTTTACAGGTTCTG30160.2660634953121306No Hit
GCCTTGGGCTGACCTAGGACGGTCAGTTTGGTCCCTCCGCCGAATATTATAGAGTTGGTGTCCCACACCTGACAG29670.2617408456867014No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAG28980.2556538492753828No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG28950.25538919725749937No Hit
GACCTAGGACGGTCAGTTTGGTCCCTCCGCCGAATATTATAGAGTTGGTGTCCCACACCTGACAGTAATAGTCAG25680.22654212730820666No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG23920.21101587559237944No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGTTTGGTCCCTC22520.1986654480911532No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCT21250.18746184600075513No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC20500.18084554555366966No Hit
GCCATGGCCTGGACCGCTCTCCTTCTGAGCCTCCTTGCTCACTTTACAGGTTCTGTGGCCTCCTATGAGCTGAC20180.17802259069624654No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAGTTGAGGGAGATGGGGTAGGTGGAGT20070.17705219996400734No Hit
CTCTCAGTGTCAGTGGCCCTGGGACAGACGGCCAGGATTCCCTGTGGGGGAAACAACATTGGAAGTAAAAATG19980.17625824391035708No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT19620.17308241969575605No Hit
GACTATTACTGTCAGGTGTGGGACACCAACTCTATAATATTCGGCGGAGGGACCAAACTGACCGTCCTAGGTCAG18980.16743650998090978No Hit
CTATAATATTCGGCGGAGGGACCAAACTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCC18940.16708364062373188No Hit
GTGTATAACTGTGCGAGAGATGGGGGGGCGGTGGCTGGTATATATAACTA18380.1621434696232414No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG18340.16179060026606348No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC18220.16073199219452983No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC18200.16055555751594086No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG17640.15561538651545037No Hit
CAGTTATACACAGCCGTGTCCTCGGCTCTCAGGTTGTTCATTTGCAGATACACCGTGTTCTTGGAATTGTCTC17300.1526159969794383No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC17220.15191025826508253No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC17070.15058699817566543No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG17020.15014591147919307No Hit
GTAATAGTCAGCCTCATCCCCGGCTTGGGCTCTGCTGATGGTCAGGGTGG16870.14882265138977596No Hit
GGCCAGGCCCCTGCGCTGGTCATATATAAAGATGACAAGAGGCCCTCTGG16850.14864621671118702No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA16740.14767582597894782No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGC16680.14714652194318098No Hit
GCGCAGGGGCCTGGCCTGCCTTCTGCTGGTACCAGTGCACATTTTTACTTCCAATGTTGTTTCCCCCACAGGGAA16590.14635256588953072No Hit
GCACTGAACACAGAGGACTCACCATGGAGTTTGGGCTGAGCTGGGTTTTC16270.14352961103210757No Hit
CCCCTGCGCTGGTCATATATAAAGATGACAAGAGGCCCTCTGGGATCCCG16210.14300030699634075No Hit
GGGTGGCCGTGTTCCCCGAGTTGGAGCCAGAGAATCGCTCCGGGATCCCAGAGGGCCTCTTGTCATCTTTATATA16170.14264743763916285No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC16130.14229456828198497No Hit
CTCCTGTGCAGCGTCTGGATTCGGCTTCAGTAATTATGGCATGCACTGGGTCCGCCAGGCTCCAGGCAAGGGG16100.14202991626410152No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATGGGTGGGTTTACCCGCCAAGCGG16020.14132417754974577No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGG15920.14044200415680103No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA15910.14035378681750654No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG15750.13894230938879498No Hit
ATATTATAGAGTTGGTGTCCCACACCTGACAGTAATAGTCAGCCTCATCC15620.13779548397796684No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA15520.1369133105850221No Hit
CCCTGGGACAGACGGCCAGGATTCCCTGTGGGGGAAACAACATTGGAAGT15290.13488431178124924No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAGTGTCCTGCCGGGCTGTGCCGAGCCA15200.13409035572759898No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG15160.13373748637042107No Hit
CTATTACTGTCAGGTGTGGGACACCAACTCTATAATATTCGGCGGAGGGACCAAACTGACCGTCCTAGGTCAGCC15010.132414226281004No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC14890.1313556182094703No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT14350.12659188188756879No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCTGACGCCTGAGCAG14260.12579792583391852No Hit
GGTCATATATAAAGATGACAAGAGGCCCTCTGGGATCCCGGAGCGATTCTCTGGCTCCAACTCGGGGAACACGGC14040.1238571443694401No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA13820.1219163629049617No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA13540.11944627740471646No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT13510.11918162538683302No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC13460.11874053869036066No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC13410.1182994519938883No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCC13390.11812301731529935No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT13360.11785836529741593No Hit
GAGTACGGGAGCCTGGGCCTCAGGAAGCAGCATCGGGGGTGCCGCAGCCATGGCCTGGACCGCTCTCCTTCTGAG13230.11671153988658779No Hit
GCATCGGGGGTGCCGCAGCCATGGCCTGGACCGCTCTCCTTCTGAGCCTCCTTGCTCACTTTACAGGTTCTG13200.11644688786870439No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT13010.11477075842210938No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT12940.11415323704704806No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG12910.11388858502916466No Hit
GTACGGGAGCCTGGGCCTCAGGAAGCAGCATCGGGGGTGCCGCAGCCATGGCCTGGACCGCTCTCCTTCTGAGC12840.11327106365410333No Hit
GGCCTGGCCTGCCTTCTGCTGGTACCAGTGCACATTTTTACTTCCAATGTTGTTTCCCCCACAGGGAATCCTGGC12760.11256532493974757No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCCGGTCGGTGCAGTGACAGTCGGGGG12750.1124771076004531No Hit
CTCTCCTTCTGAGCCTCCTTGCTCACTTTACAGGTTCTGTGGCCTCCTATGAGCTGACTCAGCCACTCTCAGTG12420.10956593540373546No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGT12270.10824267531431837No Hit
CAGTAATAGTCAGCCTCATCCCCGGCTTGGGCTCTGCTGATGGTCAGGGT12190.10753693659996258No Hit
GGATTCCCTGTGGGGGAAACAACATTGGAAGTAAAAATGTGCACTGGTACCAGCAGAAGGCAGGCCAGGCCCC12060.10639011118913444No Hit
GCTCTAGTCTCCCGTGGTGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGG12050.10630189384983997No Hit
CCTTCTGAGCCTCCTTGCTCACTTTACAGGTTCTGTGGCCTCCTATGAGCTGACTCAGCCACTCTCAGTGTCAG11900.10497863376042287No Hit
CAGTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCC11790.10400824302818368No Hit
CTCCAGCCCCTTGCCTGGAGCCTGGCGGACCCAGTGCATGCCATAATTAC11770.10383180834959473No Hit
GATCAGCACTGAACACAGAGGACTCACCATGGAGTTTGGGCTGAGCTGGGTTTTCCTCGTTGCTCTTTTAAGAGG11720.10339072165312237No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGG11640.10268498293876657No Hit
GGGCTGGAGTGGGTGGCATTTACATGGTATGATGGAAGTAATAAATACTA11610.10242033092088318No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA11510.10153815752793843No Hit
GTTTGGTCCCTCCGCCGAATATTATAGAGTTGGTGTCCCACACCTGACAGTAATAGTCAGCCTCATCCCCGGCT11350.10012668009922686No Hit
GTATCAACGCAGAGTACGGGAGCTCTGGGAGAGGAGCCCAGCACTAGAAG11350.10012668009922686No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)