FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685185

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685185
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1133564
Sequences flagged as poor quality0
Sequence length18-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTATACACAGCCGTGTCCTCGGCTCTCAGGTTGTTCATTTGCAGATACACCGTGTTCTTGGAATTGTCTCTGGA53950.4759325454936819No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGT36340.32058181099611494No Hit
CTTTACAGGTTCTGTGGCCTCCTATGAGCTGACTCAGCCACTCTCAGTGTCAGTGGCCCTGGGACAGACGGCCAG30180.2662399299907195No Hit
CCTATGAGCTGACTCAGCCACTCTCAGTGTCAGTGGCCCTGGGACAGACG29950.26421093118694666No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG29510.26032936825798986No Hit
GGCCTGGACCGCTCTCCTTCTGAGCCTCCTTGCTCACTTTACAGGTTCTG28630.2525662424000762No Hit
GCCTTGGGCTGACCTAGGACGGTCAGTTTGGTCCCTCCGCCGAATATTAT28050.2474496367209968No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT26980.2380103814164882No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG25640.2261892579510288No Hit
GACCTAGGACGGTCAGTTTGGTCCCTCCGCCGAATATTATAGAGTTGGTGTCCCACACCTGACAGTAATAGTCA23800.20995726752084573No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAG22310.1968128839659693No Hit
GCCATGGCCTGGACCGCTCTCCTTCTGAGCCTCCTTGCTCACTTTACAGGTTCTGTGGCCTCCTATGAGCTGACT20230.1784636773927189No Hit
GACTATTACTGTCAGGTGTGGGACACCAACTCTATAATATTCGGCGGAGGGACCAAACTGACCGTCCTAGGTCA20110.17740506932118522No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGTTTGGTCCCTC20050.17687576528541837No Hit
CTCTCAGTGTCAGTGGCCCTGGGACAGACGGCCAGGATTCCCTGTGGGGGAAACAACATTGGAAGTAAAAATGT19590.17281776767787263No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC19370.1708769862133942No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCA19150.1689362047489158No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT18920.1669072059451429No Hit
CTATAATATTCGGCGGAGGGACCAAACTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCC18850.16628968457008161No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC18720.16514285915925347No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT18600.1640842510877198No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATA18000.1587912107300514No Hit
CTCCAGCCCCTTGCCTGGAGCCTGGCGGACCCAGTGCATGCCATAATTACTGAAGCCGAATCCAGACGCTGCACA17510.15446856110462223No Hit
GTGTATAACTGTGCGAGAGATGGGGGGGCGGTGGCTGGTATATATAACTA17500.15438034376532775No Hit
GGCCAGGCCCCTGCGCTGGTCATATATAAAGATGACAAGAGGCCCTCTGG16680.14714652194318098No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC16560.1460879138716473No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG16400.14467643644293574No Hit
GGGTGGCCGTGTTCCCCGAGTTGGAGCCAGAGAATCGCTCCGGGATCCCA16380.1445000017643468No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC16310.14388248038928547No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGG16140.14238278562127943No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTTCCATGGCTCGGCACAGCCCGGCAG15910.14035378681750654No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAG15880.14008913479962312No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCA15860.13991270012103418No Hit
GTAATAGTCAGCCTCATCCCCGGCTTGGGCTCTGCTGATGGTCAGGGTGGCCGTGTTCCCCGAGTTGGAGCCAGA15780.1392069614066784No Hit
CAGTTATACACAGCCGTGTCCTCGGCTCTCAGGTTGTTCATTTGCAGATACACCGTGTTCTTGGAATTGTCTCTG15730.13876587471020604No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC15690.13841300535302814No Hit
CCCCTGCGCTGGTCATATATAAAGATGACAAGAGGCCCTCTGGGATCCCGGAGCGATTCTCTGGCTCCAACTCGG15670.1382365706744392No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG15500.13673687590643316No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTG15240.13444322508477685No Hit
ATATTATAGAGTTGGTGTCCCACACCTGACAGTAATAGTCAGCCTCATCCCCGGCTTGGGCTCTGCTGATGGTCA15220.1342667904061879No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA14790.1304734448165256No Hit
GCGCAGGGGCCTGGCCTGCCTTCTGCTGGTACCAGTGCACATTTTTACTT14650.12923840206640294No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA14420.12720940326263008No Hit
GGTCATATATAAAGATGACAAGAGGCCCTCTGGGATCCCGGAGCGATTCTCTGGCTCCAACTCGGGGAACACGGC14240.12562149115532956No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGG14220.12544505647674062No Hit
CTATTACTGTCAGGTGTGGGACACCAACTCTATAATATTCGGCGGAGGGACCAAACTGACCGTCCTAGGTCAGCC14170.12500396978026826No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAACGTGGTCATCGCCTGCCTGGTCCAGG14090.12429823106591247No Hit
CCCTGGGACAGACGGCCAGGATTCCCTGTGGGGGAAACAACATTGGAAGT14040.1238571443694401No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTT13980.12332784033367326No Hit
GAGTACGGGAGCCTGGGCCTCAGGAAGCAGCATCGGGGGTGCCGCAGCCATGGCCTGGACCGCTCTCCTTCTGA13960.12315140565508431No Hit
CTCCTGTGCAGCGTCTGGATTCGGCTTCAGTAATTATGGCATGCACTGGGTCCGCCAGGCTCCAGGCAAGGG13850.12218101492284512No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCT13820.1219163629049617No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGC13480.11891697336894962No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTG13470.11882875602965515No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT13430.11847588667247724No Hit
GTATTTATTACTTCCATCATACCATGTAAATGCCACCCACTCCAGCCCCTTGCCTGGAGCCTGGCGGACCCAGT13270.1170644092437657No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGG13190.1163586705294099No Hit
GGTACCAGCAGAAGGCAGGCCAGGCCCCTGCGCTGGTCATATATAAAGATGACAAGAGGCCCTCTGGGATCCCG12770.11265354227904202No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGACTTGCCGGCTAGGCACTGTGTGGCC12520.11044810879668021No Hit
CTCTCCTTCTGAGCCTCCTTGCTCACTTTACAGGTTCTGTGGCCTCCTATGAGCTGACTCAGCCACTCTCAGTGT12510.11035989145738573No Hit
GCATCGGGGGTGCCGCAGCCATGGCCTGGACCGCTCTCCTTCTGAGCCTCCTTGCTCACTTTACAGGTTCTGTG12510.11035989145738573No Hit
GTACGGGAGCCTGGGCCTCAGGAAGCAGCATCGGGGGTGCCGCAGCCATGGCCTGGACCGCTCTCCTTCTGAGC12410.109477718064441No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAG12280.10833089265361284No Hit
GCACTGAACACAGAGGACTCACCATGGAGTTTGGGCTGAGCTGGGTTTTC12250.10806624063572944No Hit
GGGCTGGAGTGGGTGGCATTTACATGGTATGATGGAAGTAATAAATACTA12200.10762515393925706No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCT12120.10691941522490128No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC12010.10594902449266208No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGTGAGGGTTGA11880.10480219908183394No Hit
GGCCTGGCCTGCCTTCTGCTGGTACCAGTGCACATTTTTACTTCCAATGTTGTTTCCCCCACAGGGAATCCTGGC11860.10462576440324499No Hit
CTGCAGCACCCAGCCAGATGGGAACGTGGTCATCGCCTGCCTGGTCCAGG11840.10444932972465604No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC11800.10409646036747815No Hit
GTCCAAGACCCCGCTAACCGCCACCCTCTCAAAATCCGGAAACACATTCCGGCCCGAGGTCCACCTGCTGCCGCC11710.10330250431382788No Hit
CCTGTACACCACGAGCAGCCAGCTGACCCTGCCGGCCACACAGTGCCTAGCCGGCAAGTCCGTGACATGCCACGT11680.10303785229594448No Hit
CAGTAATAGTCAGCCTCATCCCCGGCTTGGGCTCTGCTGATGGTCAGGGTGGCCGTGTTCCCCGAGTTGGAGCCA11650.10277320027806106No Hit
GCTCTAGTCTCCCGTGGTGGGGGTGAGGGTTGAGAACCTATGAACATTCT11440.10092063615287712No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGC11350.10012668009922686No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACGAC150.00259671167.324314
GAGCATT951.4146774E-462.25369370
TGGGGCA7050.056.6243770
GGTAGAA21250.049.40013570
AATGGTA2852.6557245E-1046.6902770
GGTATCA9450.046.0022541
GAAACAA25300.044.41419270
CTCTGGA15700.043.88781769
CGATTCA10900.043.40624670
GTGGTAT2050.042.7406041
AAAGTCA1755.6379045E-542.24357670
GTATCAA24100.039.2925721
TGACAGT48600.038.02791670
TCAGCCA28550.037.286870
TGGTATC2450.037.0937922
CCACCTT10100.036.59715770
GTTATAC14150.035.9618231
AAGCGGT10700.034.5449870
TAGGCAA3009.699779E-734.4989270
AGGGAAT13750.034.40931370
Produced by FastQC (version 0.11.5)