FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685188

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685188
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences965382
Sequences flagged as poor quality0
Sequence length18-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC37420.3876185800025275No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTAT36050.3734273064962885No Hit
CCCAAGTACTGTAAGTCTGTTGACAGTAGTAAGTTGCAAAATCTTCAGGT29520.30578568898114944No Hit
GTAATAGACGGCCGTGTCTGCGGCGGTCACAGAGGTCAGCTTCAGGGAGAACTGGTTCTTGGACGTGTCTAGTGA27170.2814429935507395No Hit
GTACTTGGGCGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCC26840.27802465759668193No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC26170.2710843997505651No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCT25510.26424772784244993No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC24690.2557536809263069No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGG24470.25347479029026854No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG23730.24580943087813942No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA22730.235450837077965No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG22550.2335862901939336No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG22270.23068588392988476No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG21780.22561017296779928No Hit
GACTTACAGTACTTGGGCGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCT21190.21949860262569634No Hit
GCATAAATCAGGGAAAGCCCCTAAGCTCCTGATCTTTGCTGCATACAATTTGCAAAGTGGGGTCCCATCAAGGT20790.21535516510562658No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAACATTAACAAGTATTTAAATTGGTATCAGCATAAA19540.2024069228554085No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCC19180.19867782908734574No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACT18720.19391287593926548No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT18460.19121964155122015No Hit
GATTTATGCTGATACCAATTTAAATACTTGTTAATGTTCTGACTTGCCCGGCAAGTGATGGTGAC16530.17122755551688348No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTT16510.17102038364087999No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT16290.1687414930048416No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAAC15960.16532315705078404No Hit
CTGTAAGTCTGTTGACAGTAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGTCGATGGTGAGAGTGAAATCTGTC15830.16397653985676136No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGCTGTGACATC15730.16294068047674393No Hit
ACTTACTACTGTCAACAGACTTACAGTACTTGGGCGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTG15620.16180123515872474No Hit
GTCTATTACTGTGCGAGAGCCACAGTGACTCCTGGAGAAGGGCGTTTGAA15610.161697649220723No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT15580.16138689140671775No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCC15480.1603510320267003No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC15210.1575542117006532No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG15160.15703628201064448No Hit
GATTTCACTCTCACCATCGACAGTCTGCAACCTGAAGATTTTGCAACTTACTACTGTCAACAGACTTACAGTACT14580.1510282976065433No Hit
GTCTGCAACCTGAAGATTTTGCAACTTACTACTGTCAACAGACTTACAGT14140.14647051633446656No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGCTG13890.14388086788442295No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13670.14160197724838458No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGCTGTGACATCCTGATGACCCAGTC13530.14015177411636015No Hit
GAGTGAAATCTGTCCCAGATCCACTGCCACTGAACCTTGATGGGACCCCACTTTGCAAATTGTATGCAGCAAAGA13400.13880515692233747No Hit
GTAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGTCGATGGTGAGAGTGAAATCTGTCCCAGATCCACTGCCA13230.13704419597630782No Hit
CACCATCGACAGTCTGCAACCTGAAGATTTTGCAACTTACTACTGTCAACAGACTTACAGTACTTGGGCGTTCGG13180.1365262662862991No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGCTGTGACA13020.13486889127827117No Hit
GTCGATGGTGAGAGTGAAATCTGTCCCAGATCCACTGCCACTGAACCTTGATGGGACCCCACTTTGCAAATTGTA12820.1327971725182363No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG12490.12937883656417873No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC12410.1285501490601648No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT12200.12637484436212815No Hit
GATCAGGAGCTTAGGGGCTTTCCCTGATTTATGCTGATACCAATTTAAAT12190.12627125842412643No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA12030.1246138834160985No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC11820.12243857871806185No Hit
GTACTGTAAGTCTGTTGACAGTAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGTCGATGGTGAGAGTGAAA11720.12140271933804442No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA11720.12140271933804442No Hit
GTATTTAAATTGGTATCAGCATAAATCAGGGAAAGCCCCTAAGCTCCTGATCTTTGCTGCATACAATTTGCAAAG11460.11870948494999906No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCA11390.11798438338398685No Hit
GTGGGGTCCCATCAAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCACT11330.11736286775597639No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT11320.11725928181797465No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGCC11110.11508397711993801No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTG11070.11466963336793103No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC10980.11373735992591533No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTT10970.11363377398791359No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAGTTGAGGGAGATGGGGTAGGTGGAGT10770.11156205522787871No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC10730.11114771147587173No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC10710.11094053959986823No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAACATTAACAAGTATTTAAATTGGTA10610.1099046802198508No Hit
GCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGCTGTGACATCCTGATGACCCAGTCTCCATCCTCC10440.10814371927382115No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA10260.10627917238978973No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG10240.10607200051378626No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT10120.10482896925776532No Hit
GTGACATCCTGATGACCCAGTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGG10110.10472538331976358No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC10090.10451821144376008No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATGGGTGGGTTTACCCGCCAAGCGG10080.10441462550575835No Hit
GCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTC10030.10389669581574962No Hit
AGTCAGAACATTAACAAGTATTTAAATTGGTATCAGCATAAATCAGGGAAAGCCCCTAAGCTCCTGATCTT9930.10286083643573217No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA9930.10286083643573217No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC9920.10275725049773043No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT9900.10255007862172694No Hit
CTCCTGATCTTTGCTGCATACAATTTGCAAAGTGGGGTCCCATCAAGGTTCAGTGGCAGTGGATCTGGGACAGAT9820.101721391117713No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGG9810.10161780517971124No Hit
ACCTTGATGGGACCCCACTTTGCAAATTGTATGCAGCAAAGATCAGGAGCTTAGGGGCTTTCCCTGATTTA9810.10161780517971124No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTT9710.10058194579969379No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA9690.10037477392369032No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA9690.10037477392369032No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCCTAG209.238838E-567.869931
AATCGAG754.272204E-665.3340370
AATGGTA4000.051.4505570
TGTTAGA11450.050.49835670
ATGGCAA1953.6379788E-1045.2312570
AAACTCA2457.6397555E-1140.00042770
GTGGTAT2050.039.7287371
CTAGTGA7350.039.3255869
CGTATGC1850.038.95887441
CCCTAGT350.001464264738.76875315
GTATCAA21500.037.7230571
GCGTAGA23750.037.13723870
TCGTATG1850.037.0745440
CGAGACG2350.036.1328623
CTTATAC2500.035.2923621
AGTAAGA1401.7077853E-435.00037470
ACGAGAC2450.034.64546622
TATTCTA1150.002751353434.08732270
GACGCTC2500.033.9772226
CCCACGA2500.033.9367219
Produced by FastQC (version 0.11.5)