FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685189

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685189
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences965382
Sequences flagged as poor quality0
Sequence length18-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTA36560.3787101893343775No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT36160.3745667518143077No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC28350.2936661342349453No Hit
CCCAAGTACTGTAAGTCTGTTGACAGTAGTAAGTTGCAAAATCTTCAGGT26030.26963419661854066No Hit
GTAATAGACGGCCGTGTCTGCGGCGGTCACAGAGGTCAGCTTCAGGGAGAACTGGTTCTTGGACGTGTCTAGT25140.2604150481363854No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG25140.2604150481363854No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG25130.26031146219838364No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTT24760.25647878249231915No Hit
GTACTTGGGCGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCC24260.25129948559223186No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGC24130.24995286839820918No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG21530.22302052451775564No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGTTA21350.2211559776337243No Hit
GACTTACAGTACTTGGGCGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTT21070.2182555713696754No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAA19370.2006459619093789No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTC19160.19847065721134224No Hit
GCATAAATCAGGGAAAGCCCCTAAGCTCCTGATCTTTGCTGCATACAATTTGCAAAGTGGGGTCCCATCAAGGTT18920.1959845946993004No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT18520.19184115717923061No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT18260.18914792279118525No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAACATTAACAA17510.18137897744105444No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCC17340.1796180164950248No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAACATTAACAA16780.17381720396692707No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT16290.1687414930048416No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT16060.16635901643080148No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGA15540.16097254765471078No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGCTGTGACATC15250.1579685554526602No Hit
GATTTATGCTGATACCAATTTAAATACTTGTTAATGTTCTGACTTGCCCGGCAAGTGATGGTGACT15200.15745062576265148No Hit
CTGTAAGTCTGTTGACAGTAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGTCGATGGTGAGAGTGAAATCTGTC15190.15734703982464973No Hit
GTCTATTACTGTGCGAGAGCCACAGTGACTCCTGGAGAAGGGCGTTTGAA15050.1558968366926253No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC14900.15434304762259912No Hit
GATTTCACTCTCACCATCGACAGTCTGCAACCTGAAGATTTTGCAACTTA14800.1533071882425817No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACT14640.1516498132345538No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACA14400.14916375072251192No Hit
ACTTACTACTGTCAACAGACTTACAGTACTTGGGCGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTG14240.147506375714484No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGCTGTGACATCCTGATGACCCAGTCTCCATCCTCCCTGTCTGCATCTGTA14110.14615975852046134No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT13940.1443987975744317No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGCTGT13720.1421199069383933No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCC13680.14170556318638633No Hit
GTCTGCAACCTGAAGATTTTGCAACTTACTACTGTCAACAGACTTACAGT13350.13828722723232878No Hit
GAGTGAAATCTGTCCCAGATCCACTGCCACTGAACCTTGATGGGACCCCACTTTGCAAATTGTATGCAGCAAA13160.13631909441029563No Hit
CACCATCGACAGTCTGCAACCTGAAGATTTTGCAACTTACTACTGTCAACAGACTTACAGTACTTGGGCGTTCG12720.13176131313821887No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA12630.13082903969620316No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG12480.129275250626177No Hit
GTGGGGTCCCATCAAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCACTCTCACCATCGACAGTCTGCAACCT12260.1269963599901386No Hit
GTCGATGGTGAGAGTGAAATCTGTCCCAGATCCACTGCCACTGAACCTTG12260.1269963599901386No Hit
GTAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGTCGATGGTGAGAGTGAAATCTGTCCCAGATCCACTGCCA12230.1266856021761334No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGCTGTGACAT12030.1246138834160985No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA11520.11933100057800954No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC11490.1190202427640043No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCT11330.11736286775597639No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGA11160.11560190680994674No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT10820.11207998491788744No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT10780.11166564116588044No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTAC10750.11135488335187521No Hit
GCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGCTGTGACATCCTGATGACCCAGTCTCCATCCTCC10720.11104412553786998No Hit
CTCCTGATCTTTGCTGCATACAATTTGCAAAGTGGGGTCCCATCAAGGTT10690.11073336772386476No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA10660.11042260990985953No Hit
GTACTGTAAGTCTGTTGACAGTAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGTCGATGGTGAGAGTGAAAT10630.11011185209585429No Hit
GATCAGGAGCTTAGGGGCTTTCCCTGATTTATGCTGATACCAATTTAAATACTTGTTAATGTTCTGACTTGCCCG10620.11000826615785253No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAA10510.10886882083983335No Hit
GTATTTAAATTGGTATCAGCATAAATCAGGGAAAGCCCCTAAGCTCCTGA10420.10793654739781765No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA10230.1059684145757845No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGG10190.10555407082377753No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT10100.10462179738176182No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC10030.10389669581574962No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA10020.10379310987774788No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTA9890.1024464926837252No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGACTTGCCGGCTAGGCACTGTGTGGCC9790.10141063330370775No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGCGT404.7957973E-4102.9898570
GTTATTA901.6998893E-676.2887970
TATTCTA1157.284383E-659.7042670
AATGGTA3900.042.25224770
TAATACC504.346437E-640.66370820
CATAAAT15550.040.62193770
AAGGACA24250.040.20497570
TAAGTCA1758.7140514E-539.2342370
GAGTGAA20750.038.38336670
CTGGAGT9500.037.5822670
GTATCAA20150.036.4741441
GAGCAAA21550.036.3212570
ACTGTAA18650.035.34236570
TGCCCGA400.002437188334.94718657
GTGGTAT2150.034.6564941
TAACCCG501.8518115E-434.5296148
CAATAGA601.5081319E-533.918126
AAGCGGT8250.033.28965470
GACAGGT2050.033.042781
AGTAATA6700.032.7927970
Produced by FastQC (version 0.11.5)