FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685192

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685192
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences363092
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC29470.811640025117601No Hit
ACCTAGGACAGTGACCTTGGTCCCTCTTCCGAAGACATATGTATCACTAC19900.5480704614808368No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAG19780.5447655139744197No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC19670.541735978760204No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACAGTGACCTTGGTCCC16550.4558073435933592No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAG15050.4144954997631454No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG14870.40953807850351975No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC14680.40430524495169273No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC13540.37290824364073016No Hit
GTTATAACCTTGTCTCCTGGTACCAACAGGACGCAGGCAAAGCCCCCAAA12350.3401341808687605No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCC12200.33600299648573917No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT11330.31204212706421514No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCT10830.29827151245414385No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGG10750.29606821411653245No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACAGTGACCTTGGTCCCTCTTCCGAAGACATATGTATCA10700.2946911526555253No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC10400.28642878388948256No Hit
GGACACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC10350.28505172242847543No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCACTGGAACCAGCAGTGATGTT9460.26054002842254853No Hit
GACCTAGGACAGTGACCTTGGTCCCTCTTCCGAAGACATATGTATCACTACTTGCAAATGAGCAGCAATAATAA9460.26054002842254853No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG9450.2602646161303471No Hit
GATTATTATTGCTGCTCATTTGCAAGTAGTGATACATATGTCTTCGGAAGAGGGACCAAGGTCACTGTCCTAGG9250.2547563702863186No Hit
GTCGATCACCATCTCCTGCACTGGAACCAGCAGTGATGTTGGGAGTTATAACCTTGTCTCCTGGTACCAACAGGA9170.25255307194870724No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG8830.24318905401385874No Hit
CTCCTGCACTGGAACCAGCAGTGATGTTGGGAGTTATAACCTTGTCTCCTGGTACCAACAGGACGCAGGCAAAGC8750.24098575567624733No Hit
GCCTTGGGCTGACCTAGGACAGTGACCTTGGTCCCTCTTCCGAAGACATATGTATCACTACTTGCAAATGAGCAG8710.23988410650744163No Hit
GACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCCTGCACTGGAACCAGCAGTGATGT8590.23657915900102455No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGC7570.20848710519647912No Hit
GGTTATAACTCCCAACATCACTGCTGGTTCCAGTGCAGGAGATGGTGATC7500.20655921915106917No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGC7410.20408050852125634No Hit
ATAATAATCAGCCTCGTCCTCAGCCTGGAGCGCAGAGATTGTCAGGGAGG7340.20215262247584637No Hit
GTACCAACAGGACGCAGGCAAAGCCCCCAAATTAATAATTTTTGAGGGCACTAAGCGGCCCTCAGGGGTTTCTAA7310.20132638559924204No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGA7270.20022473643043637No Hit
GGCCTGGGCTCTGCTGCTCCTCACTCTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGC7260.1999493241382349No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGTGACT7130.19636896433961643No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC6820.18783118328137222No Hit
CTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTG6640.18287376202174654No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGT6600.18177211285294084No Hit
AGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA6590.18149670056073944No Hit
CCTCAGGGGTTTCTAATCGCTTCTCTGGCTCCAAGTCTGGCAACACGGCCTCCCTGACAATCTCTGCGCTCCAGG6540.1801196390997323No Hit
CTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCC6180.170204796580481No Hit
CTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCCCG6020.16579819990525818No Hit
GGCAAAGCCCCCAAATTAATAATTTTTGAGGGCACTAAGCGGCCCTCAGG5950.16387031385984818No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG5850.16111619093783394No Hit
CCCCTGAGGGCCGCTTAGTGCCCTCAAAAATTATTAATTTGGGGGCTTTGCCTGCGTCCTGTTGGTACCAGGAGA5840.1608407786456325No Hit
CCCGTGGGTGGGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCA5810.16001454176902824No Hit
GACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTT5740.15808665572361824No Hit
CAATAATACACGGCCGTGTCCGCGGCAGTCACAGAGGTCAGCTTCAGGGAGAACTGATTCTTAGACGTGTCTC5720.1575358311392154No Hit
CCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGG5710.157260418847014No Hit
GCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCCTGCACTGGAACCAGCAG5640.155332532801604No Hit
CAAGAGGCAGCGCTCTCGGGACGTCTCCACCATGGCCTGGGCTCTGCTGC5490.15120134841858263No Hit
GTGATACATATGTCTTCGGAAGAGGGACCAAGGTCACTGTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCAC5460.15037511154197833No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT5430.14954887466537406No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGG5420.14927346237317263No Hit
CTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCC5350.14734557632776266No Hit
GGGCTCTGCTGCTCCTCACTCTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCC5340.14707016403556122No Hit
GGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGT5320.1465193394511584No Hit
CTCTCGGGACGTCTCCACCATGGCCTGGGCTCTGCTGCTCCTCACTCTCCTCACTCAGGACACAGGGTCCTGGGC5250.1445914534057484No Hit
GTATCAACGCAGAGTACGGGGGGGGTCACAAGAGGCAGCGCTCTCGGGACGTCTCCACCATGGCCTGGGCTC5200.14321439194474128No Hit
CATATGTATCACTACTTGCAAATGAGCAGCAATAATAATCAGCCTCGTCC5170.14238815506813698No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGT5150.14183733048373415No Hit
AAGCGGCCCTCAGGGGTTTCTAATCGCTTCTCTGGCTCCAAGTCTGGCAACACGGCCTCCCTGACAATCTCTGCG5150.14183733048373415No Hit
ACCATGGCCTGGGCTCTGCTGCTCCTCACTCTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTCTGCCCTGAC5090.1401848567305256No Hit
GGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTTGTTGGCTTGGAG5070.13963403214612274No Hit
CTGTTGGTACCAGGAGACAAGGTTATAACTCCCAACATCACTGCTGGTTCCAGTGCAGGAGATGGTGATCGA5050.1390832075617199No Hit
CTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGA5050.1390832075617199No Hit
AGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCG5030.13853238297731704No Hit
GACAATCTCTGCGCTCCAGGCTGAGGACGAGGCTGATTATTATTGCTGCTCATTTGCAAGTAGTGATACATATG5030.13853238297731704No Hit
GGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGGAAC4980.1371553215163099No Hit
CTGCTGCTCCTCACTCTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTG4970.1368799092241085No Hit
CCACCAAACCCTCCAAACAGAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCTGACGCCCGAGCAG4960.13660449693190707No Hit
CCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAG4960.13660449693190707No Hit
ATGTATCACTACTTGCAAATGAGCAGCAATAATAATCAGCCTCGTCCTCA4800.13219790025668426No Hit
GGCCACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCC4770.13137166338007997No Hit
ACATATGTCTTCGGAAGAGGGACCAAGGTCACTGTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTC4720.12999460191907286No Hit
GAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG4710.12971918962687143No Hit
GGACGAGGCTGATTATTATTGCTGCTCATTTGCAAGTAGTGATACATATGTCTTCGGAAGAGGGACCAAGGTCA4640.12779130358146143No Hit
CCATTATCCCTTGGCCCCAGATATCAAAAGCATCGAGGTCCCCCGGAGGCATTCTCGCACAATAATACACGGCCG4530.12476176836724577No Hit
GTTATAACTCCCAACATCACTGCTGGTTCCAGTGCAGGAGATGGTGATCG4510.12421094378284292No Hit
GCCATCTGCCTTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTTGT4330.11925352252321725No Hit
GACATATGTATCACTACTTGCAAATGAGCAGCAATAATAATCAGCCTCGTCCTCAGCCTGGAGCGCAGAGATTG4330.11925352252321725No Hit
GTCTCTGGTGACTCCATCAGCAGGGGTGGTTACTACTGGAGCTGGATACGCCAGCACCCAGGGAAGGGCCTGGAG4280.11787646106221014No Hit
CTCGGGACGTCTCCACCATGGCCTGGGCTCTGCTGCTCCTCACTCTCCTC4260.11732563647780726No Hit
GGTACCAACAGGACGCAGGCAAAGCCCCCAAATTAATAATTTTTGAGGGC4220.11622398730900156No Hit
GGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAG4190.1153977504323973No Hit
GCTCGGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCA4190.1153977504323973No Hit
TTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCA4190.1153977504323973No Hit
GTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTT4160.11457151355579302No Hit
GCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCCTGCACTGGAACCAGCAGTGATGTTGGGAG4150.1142961012635916No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACT4120.11346986438698732No Hit
GGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC4110.1131944520947859No Hit
TACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTT4100.11291903980258447No Hit
GGACCAAGGTCACTGTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAG4060.11181739063377877No Hit
GTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCA4060.11181739063377877No Hit
ATAATACACGGCCGTGTCCGCGGCAGTCACAGAGGTCAGCTTCAGGGAGA4060.11181739063377877No Hit
ATACATATGTCTTCGGAAGAGGGACCAAGGTCACTGTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGT4010.11044032917277163No Hit
GCAATAATAATCAGCCTCGTCCTCAGCCTGGAGCGCAGAGATTGTCAGGG3980.10961409229616736No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT3940.10851244312736166No Hit
GAGTTATAACCTTGTCTCCTGGTACCAACAGGACGCAGGCAAAGCCCCCA3840.1057583202053474No Hit
CGTCTCCACCATGGCCTGGGCTCTGCTGCTCCTCACTCTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTCTGC3840.1057583202053474No Hit
GGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGC3810.10493208332874313No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGC3800.10465667103654169No Hit
GGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGG3800.10465667103654169No Hit
GGAGTGGAGACCACCAAACCCTCCAAACAGAGCAACAACAAGTACGCGGC3790.10438125874434029No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA3780.10410584645213884No Hit
CTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG3750.10327960957553459No Hit
AGGCAGCGCTCTCGGGACGTCTCCACCATGGCCTGGGCTCTGCTGCTCCTCACTCTCCTCACTCAGGACACAGGG3740.10300419728333314No Hit
CTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAG3710.10217796040672888No Hit
GTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGTGACT3710.10217796040672888No Hit
GGGTTGGCCTTGGGCTGACCTAGGACAGTGACCTTGGTCCCTCTTCCGAAGACATATGTATCACTACTTGCAAA3710.10217796040672888No Hit
GACACACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCT3700.10190254811452745No Hit
GATCAGACACACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGT3690.10162713582232603No Hit
GTGCAGGAGATGGTGATCGACTGTCCAGGAGACCCAGACACGGAGGCAGG3690.10162713582232603No Hit
AGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTGGA3680.1013517235301246No Hit
ACCTGAGCCTGACGCCCGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCACCG3660.10080089894572175No Hit
CCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAG3650.1005254866535203No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG3650.1005254866535203No Hit
GAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAG3650.1005254866535203No Hit
GCACTAAGCGGCCCTCAGGGGTTTCTAATCGCTTCTCTGGCTCCAAGTCTGGCAACACGGCCTCCCTGACAATC3640.1002500743613189No Hit
GTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCA3640.1002500743613189No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACTGTA304.5319143E-4104.4330470
AGGGGGG752.9818693E-883.5464370
TCGGAGG400.001421408778.32477670
GCACATC150.00233730669.1255650
TCCTGAA2200.061.71043470
TAATGCT200.006996219552.4084160
GTTAGTT200.007609763651.30614537
ATTACAC200.007752307751.06593
GTCTCCC305.789626E-446.80355563
GCTTGTC306.7193434E-445.4106751
GTGGCCG3250.044.98653470
GTGGTAT804.0017767E-1142.5725061
GTATCAA7100.041.2533571
ATGCTAG350.001245304640.06044862
AATGCTA350.001249653340.03206661
AACAAGG15550.038.9525270
GGTATCA2400.038.3152541
TATCAAC7650.038.271612
TGGTATC901.4188117E-1037.8265952
CCCGTCA9550.037.18034770
Produced by FastQC (version 0.11.5)