FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004685193

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685193
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences363092
Sequences flagged as poor quality0
Sequence length18-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAG25270.6959668623930023No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT18140.4995978980533859No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC18120.4990470734689831No Hit
ACCTAGGACAGTGACCTTGGTCCCTCTTCCGAAGACATATGTATCACTAC17010.46847630903462484No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACAGTGACCTTGGTCCCT14790.40733478016590835No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG14540.40044947286087273No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC13400.3690524715499102No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC12930.3561080938164432No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGT12920.3558326815242418No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGG12260.3376554702389477No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCCT11600.3194782589536536No Hit
GTTATAACCTTGTCTCCTGGTACCAACAGGACGCAGGCAAAGCCCCCAAATTAATAATTTTTGAGGGCACTAAGC11460.3156224868628337No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT11070.30488140746697806No Hit
GGACACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC10080.27761559053903695No Hit
GTCGATCACCATCTCCTGCACTGGAACCAGCAGTGATGTTGGGAGTTATAACCTTGTCTCCTGGTACCAACAG9700.26714992343538274No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCACTGGAACCAGCAGTGATGTT9630.2652220373899728No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT9560.2632941513445628No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC9470.26081544071475No Hit
GATTATTATTGCTGCTCATTTGCAAGTAGTGATACATATGTCTTCGGAAGAGGGACCAAGGTCACTGTCCTAGGT9280.25558260716292286No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACAGTGACCTTGGTCCCTCTTCCGAAGACATATGTATCA9130.2514514227799015No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGT9020.24842188756568584No Hit
GCCTTGGGCTGACCTAGGACAGTGACCTTGGTCCCTCTTCCGAAGACATATGTATCACTACTTGCAAATGAGCA8970.2470448261046787No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTA8840.24346446630606017No Hit
CTCCTGCACTGGAACCAGCAGTGATGTTGGGAGTTATAACCTTGTCTCCTGGTACCAACAGGACGCAGGCAAAGC8550.23547750983221885No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG8200.22583807960516894No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGTGACTTCTACCCGG8070.2222577198065504No Hit
GACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCT8030.2211560706377447No Hit
GACCTAGGACAGTGACCTTGGTCCCTCTTCCGAAGACATATGTATCACTACTTGCAAATGAGCAGCAATAATAAT7890.21730029854692473No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCT7400.2038050962290549No Hit
GGCCTGGGCTCTGCTGCTCCTCACTCTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGC7360.2027034470602492No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTG7250.19967391184603353No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC7110.19581813975521356No Hit
GTACCAACAGGACGCAGGCAAAGCCCCCAAATTAATAATTTTTGAGGGCA7070.19471649058640786No Hit
GTATCAACGCAGAGTACGGGGGGGGTCACAAGAGGCAGCGCTCTCGGGACGTCTCCACCATGGCCTGGGCTCT6890.1897590693267822No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC6810.18755577098917078No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTT6720.18507706035935798No Hit
CTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAG6620.1823229374373437No Hit
AGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCT6390.1759884547167109No Hit
GGTTATAACTCCCAACATCACTGCTGGTTCCAGTGCAGGAGATGGTGATCGACTGTCCAGGAGACCCAGACACG6230.17158185804148812No Hit
ATAATAATCAGCCTCGTCCTCAGCCTGGAGCGCAGAGATTGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCAGA6160.16965397199607812No Hit
CCTCAGGGGTTTCTAATCGCTTCTCTGGCTCCAAGTCTGGCAACACGGCCTCCCTGACAATCTCTGCGCTCCAG6070.1671752613662653No Hit
CCCCTGAGGGCCGCTTAGTGCCCTCAAAAATTATTAATTTGGGGGCTTTGCCTGCGTCCTGTTGGTACCAGGA6000.16524737532085532No Hit
CTGCTGCTCCTCACTCTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTC5960.16414572615204961No Hit
CTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCCCGTCAAG5950.16387031385984818No Hit
CCCGTGGGTGGGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCA5910.16276866469104248No Hit
ACCTGAGCCTGACGCCCGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC5910.16276866469104248No Hit
GCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCCTGCACTGGAACCAGCAGT5780.15918830489242394No Hit
GGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGGAAC5730.15781124343141684No Hit
CTCTCGGGACGTCTCCACCATGGCCTGGGCTCTGCTGCTCCTCACTCTCCTCACTCAGGACACAGGGTCCTGGGC5620.15478170821720116No Hit
ACCATGGCCTGGGCTCTGCTGCTCCTCACTCTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTCTGCCCTGACT5610.15450629592499973No Hit
GTGATACATATGTCTTCGGAAGAGGGACCAAGGTCACTGTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACT5540.15257840987958976No Hit
CTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCCCG5510.15175217300298546No Hit
GGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGC5500.15147676071078406No Hit
CTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTG5480.1509259361263812No Hit
ATGTATCACTACTTGCAAATGAGCAGCAATAATAATCAGCCTCGTCCTCAGCCTGGAGCGCAGAGATTGTCAGG5450.15009969924977692No Hit
GGGCTCTGCTGCTCCTCACTCTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCT5390.14844722549656836No Hit
AGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCCTGCACTGGAACCA5350.14734557632776266No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGG5260.14486686569794982No Hit
GACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTG5130.14128650589933128No Hit
GGCAAAGCCCCCAAATTAATAATTTTTGAGGGCACTAAGCGGCCCTCAGG5100.14046026902272704No Hit
CAAGAGGCAGCGCTCTCGGGACGTCTCCACCATGGCCTGGGCTCTGCTGC5070.13963403214612274No Hit
CTCGGGACGTCTCCACCATGGCCTGGGCTCTGCTGCTCCTCACTCTCCTC5010.1379815583929142No Hit
CCACCAAACCCTCCAAACAGAGCAACAACAAGTACGCGGCCAGCAGCTAC5010.1379815583929142No Hit
CTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACT4910.13522743547089994No Hit
CAATAATACACGGCCGTGTCCGCGGCAGTCACAGAGGTCAGCTTCAGGGAGAACTGATTCTTAGACGTGTCTC4870.13412578630209424No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAG4860.1338503740098928No Hit
AAGCGGCCCTCAGGGGTTTCTAATCGCTTCTCTGGCTCCAAGTCTGGCAACACGGCCTCCCTGACAATCTCTGC4860.1338503740098928No Hit
CCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGG4840.13329954942548997No Hit
GGCCACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCC4820.1327487248410871No Hit
CATATGTATCACTACTTGCAAATGAGCAGCAATAATAATCAGCCTCGTCCTCAGCCTGGAGCGCAGAGATTGTCA4790.13192248796448283No Hit
GGACGAGGCTGATTATTATTGCTGCTCATTTGCAAGTAGTGATACATATGTCTTCGGAAGAGGGACCAAGGTCA4620.1272404789970586No Hit
CTGTTGGTACCAGGAGACAAGGTTATAACTCCCAACATCACTGCTGGTTCCAGTGCAGGAGATGGTGATC4560.12558800524385003No Hit
ACATATGTCTTCGGAAGAGGGACCAAGGTCACTGTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTC4500.12393553149064149No Hit
TTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCA4410.12145682086082865No Hit
GCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCCTGCA4400.12118140856862723No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG4370.12035517169202295No Hit
GACAATCTCTGCGCTCCAGGCTGAGGACGAGGCTGATTATTATTGCTGCTCATTTGCAAGTAGTGATACATATGT4310.1187026979388144No Hit
GTCTCTGGTGACTCCATCAGCAGGGGTGGTTACTACTGGAGCTGGATACG4170.11484692584799445No Hit
GGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGA4150.1142961012635916No Hit
GTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCA4140.11402068897139017No Hit
ATACATATGTCTTCGGAAGAGGGACCAAGGTCACTGTCCTAGGTCAGCCC4080.11236821521818162No Hit
CGTCTCCACCATGGCCTGGGCTCTGCTGCTCCTCACTCTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTCTGC4080.11236821521818162No Hit
CAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGG4050.11154197834157734No Hit
GGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCA4030.11099115375717448No Hit
GAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCTGACGCCCGAGCAGTGGAAGTCCCACAGAAGCTACA3910.10768620625075738No Hit
TACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG3880.10685996937415312No Hit
GAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCA3880.10685996937415312No Hit
AGGCAGCGCTCTCGGGACGTCTCCACCATGGCCTGGGCTCTGCTGCTCCT3880.10685996937415312No Hit
GGACCAAGGTCACTGTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGA3860.10630914478975025No Hit
GACACACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGG3840.1057583202053474No Hit
CTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACA3810.10493208332874313No Hit
GAGTTATAACCTTGTCTCCTGGTACCAACAGGACGCAGGCAAAGCCCCCAAATTAATAATTTTTGAGGGCACTAA3800.10465667103654169No Hit
CTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCAAACCCTCCAAACAGA3750.10327960957553459No Hit
CTCAGGACACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTC3740.10300419728333314No Hit
GCCATCTGCCTTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTTGTT3740.10300419728333314No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACACCTGTGGTTCTTCCTCCTGCTGGTGGCAGCTCCCAGATGGGTC3730.10272878499113174No Hit
GGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGC3730.10272878499113174No Hit
GGCCTGGAAGGCAGATGGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCAAACCCTCCAAACAGAGCAACAACAA3700.10190254811452745No Hit
ACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGG3690.10162713582232603No Hit
CCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGGAACCTATGAACATTCTGTAG3680.1013517235301246No Hit
CCATTATCCCTTGGCCCCAGATATCAAAAGCATCGAGGTCCCCCGGAGGC3670.10107631123792318No Hit
ATAATACACGGCCGTGTCCGCGGCAGTCACAGAGGTCAGCTTCAGGGAGA3650.1005254866535203No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)