FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685194

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685194
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences919710
Sequences flagged as poor quality0
Sequence length18-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTAAGCTGTTGACAGTAATAAGTTGCAAAATCTTCAGGCTGCAGGCTGCTGATTGTGAGAGTGAATTCTGTC66870.7270770134063999No Hit
GCTTAATACTTACCCCCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCA56830.6179121679659892No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC35100.3816420393384871No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTAT34910.37957617074947536No Hit
GATCTATGGTGCATCCACTTTGCAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGAATCTGGGACAGAATTCA33060.3594611344880452No Hit
CCATAGATCAGGGGCTTAGGGGCTTTACCTGGTTTTTGCTGATACCAGGCTAAATAGCTACTAATGCCCTGAC28190.3065096606539018No Hit
GTCCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGG27820.30248665340161573No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCT25210.2741081427841385No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC23470.2551891357058203No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC23280.25312326711680855No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG23080.2509486686020594No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG23050.25062247882484695No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG21360.23224712137521608No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA20800.22615824553391828No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCC19980.21724239162344652No Hit
GTCTCCATCCTTCCTGTCTGCATCTGTAGGAGAGAGAGTCACCATCACTTGCCGGGCCAGTCAGGGCATTAGTAG19780.21506779310869728No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG19730.21452414348001No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG19620.21332811429689794No Hit
GGGCATTAGTAGCTATTTAGCCTGGTATCAGCAAAAACCAGGTAAAGCCCCTAAGCCCCTGATCTATGGTGCATC18860.2050646399408509No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT18550.20169401224298966No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACT18480.2009329027628274No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAGATG17400.18919007078318165No Hit
GTGGATGCACCATAGATCAGGGGCTTAGGGGCTTTACCTGGTTTTTGCTGATACCAGGCTAAATAGCTACTAA17370.18886388100596926No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC16800.182666275238934No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAAC16520.17962183731828513No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT16140.1754901001402616No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAGATGTGACAC15990.1738591512541997No Hit
GTGCATCCACTTTGCAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGAATCTGGGACAGAATTCACTCTCACAA14700.15983299083406727No Hit
GTAGGAGAGAGAGTCACCATCACTTGCCGGGCCAGTCAGGGCATTAGTAGCTATTTAGCCTGGTATCAGCAAAAA14120.15352665514129454No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG13990.15211316610670755No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTG13800.1500472975176958No Hit
GTAATAGACGGCCGTGTCCTCGGTTTTCAGGCTGTTCATTTCCAAAAACA13690.14885126833458373No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC13640.14830761870589643No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA13540.14722031944852182No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13510.14689412967130944No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT13330.14493699100803514No Hit
GAATTCACTCTCACAATCAGCAGCCTGCAGCCTGAAGATTTTGCAACTTATTACTGTCAACAGCTTAATACTTAC13230.14384969175066054No Hit
TTATTACCCTGACTACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAGCATCCCCGACCAGCCCCAAGGTCT13090.14232747279033608No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAGATGTGACACCCAGTTGACCCAGTCTCCATCCTTC12820.13939176479542464No Hit
CAGTTGACCCAGTCTCCATCCTTCCTGTCTGCATCTGTAGGAGAGAGAGT12760.13873938524099988No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT12580.13678224657772559No Hit
GGGTAATAACGTCCACCAACATAATACAGTAATCTGACACAGTAATAGACGGCCGTGTCCTCGGTTTTCAGG12560.13656478672625066No Hit
GTAATAAGTTGCAAAATCTTCAGGCTGCAGGCTGCTGATTGTGAGAGTGAATTCTGTCCCAGATTCACTGCCG12430.13515129769166367No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG12360.13439018821150145No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC12260.13330288895412684No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC12120.1317806699938024No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCA11980.13025845103347794No Hit
CTGTAGGAGAGAGAGTCACCATCACTTGCCGGGCCAGTCAGGGCATTAGT11920.12960607147905318No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC11810.1284100422959411No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTG11450.12449576496939253No Hit
ACTTATTACTGTCAACAGCTTAATACTTACCCCCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAA11390.12384338541496775No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC11380.1237346554892303No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA11290.12275608615759316No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCC11180.12156005697448108No Hit
GTGGGGTCCCATCAAGGTTCAGCGGCAGTGAATCTGGGACAGAATTCACT11180.12156005697448108No Hit
ACCTTGATGGGACCCCACTTTGCAAAGTGGATGCACCATAGATCAGGGGCTTAGGGGCTTTACCTGGTTTT11140.12112513727153124No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT11080.12047275771710647No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTT11040.12003783801415664No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC10810.11753704972219504No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG10670.11601483076187058No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC10420.11329658261843406No Hit
CTAATGCCCTGACTGGCCCGGCAAGTGATGGTGACTCTCTCTCCTACAGA10330.11231801328679693No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT10180.110687064400735No Hit
ATACTTACCCCCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCA10030.1090561155146731No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC10030.1090561155146731No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC9870.10731643670287375No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA9720.10568548781681182No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTAGGTGGGAAGTTTCTGGCGGT9710.10557675789107436No Hit
GATCAGGGGCTTAGGGGCTTTACCTGGTTTTTGCTGATACCAGGCTAAAT9670.1051418381881245No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGG9640.10481564841091215No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTT9620.10459818855943723No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA9600.1043807287079623No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA9590.10427199878222483No Hit
GAGTACGGGAGTCCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGC9570.10405453893074991No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC9460.10285850974763784No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA9350.10166248056452579No Hit
GGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAGATGTGACACCCAGT9330.10144502071305085No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA9290.10101010101010101No Hit
GACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCC9210.10014026160420132No Hit
GACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCT9210.10014026160420132No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCTAA500.003757239661.32060670
AATGGTA2900.052.8625970
TACCGGT200.00782069750.9588136
TCTTGAG6350.049.8934170
CGGCAAA1301.5619789E-647.16969770
AACTGTA906.708934E-445.42267270
GTGGTAT1900.044.710441
TCGTATG1400.044.10582440
CGTATGC1550.042.08537341
TGTTAGA9550.041.7365470
CTAGGGA1000.001126965240.88040570
AATCTCG1350.040.5281736
TCAATAG350.001262182739.95760759
GTATCAA16150.039.765991
GAGCAAA14250.038.72880670
ATGCCGT1600.038.70734444
ACGAGAC1800.037.77396822
TTATACA1900.037.5465622
CGTCGGA1658.0442605E-637.16400570
TGAGCGG3600.036.90592270
Produced by FastQC (version 0.11.5)