FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685195

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685195
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences919710
Sequences flagged as poor quality0
Sequence length18-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTAAGCTGTTGACAGTAATAAGTTGCAAAATCTTCAGGCTGCAGGCTGCTGATTGTGAGAGTGAATTCTGTC64570.7020691304867839No Hit
GCTTAATACTTACCCCCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT52320.5688749714583945No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT35620.3872959954768351No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTA34250.372399995650803No Hit
GATCTATGGTGCATCCACTTTGCAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGAATCTGGGACAGAATTCA30590.3326048428308923No Hit
GTCCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG26720.29052636157049505No Hit
CCATAGATCAGGGGCTTAGGGGCTTTACCTGGTTTTTGCTGATACCAGGCTAAATAGCTACTAATGCCCTGACTG25450.27671766100183753No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTT24080.2618216611758054No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC23870.25953833273531873No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGC22500.24464233290928664No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG21050.2288764936773548No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGTTA19920.21659001206902176No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTC19750.21474160333148493No Hit
GTCTCCATCCTTCCTGTCTGCATCTGTAGGAGAGAGAGTCACCATCACTTGCCGGGCCAGTCAGGGCATTAGTA19630.21343684422263537No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG19350.2103924063019865No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCC18910.20560828956953822No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT18830.20473845016363854No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAA18670.20299877135183916No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT18100.1968011655848039No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG17980.19549640647595437No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAGATGT17920.1948440269215296No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCA17710.19256069848104296No Hit
GGGCATTAGTAGCTATTTAGCCTGGTATCAGCAAAAACCAGGTAAAGCCCCTAAGCCCCTGATCTATGGTGCATC17560.19092974959498102No Hit
GTGGATGCACCATAGATCAGGGGCTTAGGGGCTTTACCTGGTTTTTGCTGATACCAGGCTAAATAGCTACTAAT17250.18755912189711976No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT16330.17755596872927334No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT15590.16950995422470128No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACT15140.1646171075665155No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGA15120.16439964771504062No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAGATGTGACAC14530.15798458209653043No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACA13950.1516782464037577No Hit
GTGCATCCACTTTGCAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGAA13820.15026475736917072No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTG13820.15026475736917072No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA13540.14722031944852182No Hit
GAATTCACTCTCACAATCAGCAGCCTGCAGCCTGAAGATTTTGCAACTTATTACTGTCAACAGCTTAATACTTAC13100.14243620271607355No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT12880.1400441443498494No Hit
GTAGGAGAGAGAGTCACCATCACTTGCCGGGCCAGTCAGGGCATTAGTAGCTATTTAGCCTGGTATCAGCAAAAA12640.13743462613215035No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC12620.13721716628067543No Hit
GTAATAGACGGCCGTGTCCTCGGTTTTCAGGCTGTTCATTTCCAAAAACAGTGAGTTCTTTGAATCATCTCTTGA12540.13634732687477574No Hit
GACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAGATGTGACACCCAGTT12520.13612986702330082No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAGATGTGACACCCAGTTGACCCAGTCTCCATCCTTC12100.13156321014232747No Hit
GGGTAATAACGTCCACCAACATAATACAGTAATCTGACACAGTAATAGACGGCCGTGTCCTCGGTTTTCAG11790.1281925824444662No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCA11780.12808385251872875No Hit
TTATTACCCTGACTACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAGCATCCCCGACCAGCCCCAAGGTCTT11770.12797512259299126No Hit
GTGGGGTCCCATCAAGGTTCAGCGGCAGTGAATCTGGGACAGAATTCACTCTCACAATCAGCAGCCTGCAGCCT11730.12754020289004142No Hit
GTAATAAGTTGCAAAATCTTCAGGCTGCAGGCTGCTGATTGTGAGAGTGAATTCTGTCCCAGATTCACTGCC11620.12634417370692935No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG11320.12308227593480553No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT11190.12166878690021854No Hit
CTGTAGGAGAGAGAGTCACCATCACTTGCCGGGCCAGTCAGGGCATTAGT11170.12145132704874362No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGG11000.1196029183112068No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAA10910.11862434897956964No Hit
ACTTATTACTGTCAACAGCTTAATACTTACCCCCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAA10580.11503626143023345No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCT10420.11329658261843406No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTAC10380.11286166291548422No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT10310.112100553435322No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC10290.11188309358384708No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA10210.1110132541779474No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA10200.11090452425220994No Hit
CAGTTGACCCAGTCTCCATCCTTCCTGTCTGCATCTGTAGGAGAGAGAGTCACCATCACTTGCCGGGCCAGTCAG10170.11057833447499754No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTA10150.11036087462352262No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT9940.10807754618303594No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC9710.10557675789107436No Hit
ACCTTGATGGGACCCCACTTTGCAAAGTGGATGCACCATAGATCAGGGGCTTAGGGGCTTTACCTGGTTTTT9640.10481564841091215No Hit
GTGTGACCTGGAGCGAAAGCGGACAGAACGTGACCGCCAGAAACTTCCCACCTAGCCAGGATGCCTCCGGGGAC9620.10459818855943723No Hit
GAGTACGGGAGTCCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCT9600.1043807287079623No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA9480.10307596959911276No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC9460.10285850974763784No Hit
GGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAGATGTGACACCCAGTT9420.10242359004468798No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC9370.10187994041600071No Hit
ATACTTACCCCCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGA9360.10177121049026325No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCA9200.10003153167846386No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGAGTA455.333692E-4100.2684470
CGGCAAA1258.238749E-872.19327570
TAAGTCA951.2993196E-463.3274370
GAGAAAT951.2993196E-463.3274370
CCCACAA1001.67455E-460.1610670
GTGGTAT2150.048.9134861
CAATAGT5450.044.15490770
TTGCTTA504.340851E-640.6710243
GTATCAA18100.039.1717641
TGCATTT1959.6394404E-538.56478570
ACTTCCA4307.2759576E-1238.47509870
TCACAAT15350.037.23322770
GTGTATA558.298073E-637.0078621
TACCAAA2051.293903E-436.68357570
GAAGTTT7150.035.7600770
AAGGACA23700.035.5381870
AATGGTA2651.4620418E-534.05343270
GACAGGT2500.033.923871
TATCAAC20800.033.8962062
TATAGGA400.0028308133.8962062
Produced by FastQC (version 0.11.5)