FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685196

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685196
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences883441
Sequences flagged as poor quality0
Sequence length19-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGCTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGG33860.38327403867377674No Hit
CCACAAGAGTGGTGGTTTTTGTATATGAGCTGCAATAATAATCAGCCTCGTCGTCAGGTTGGAGCCCAGAGATGG29640.33550627602748795No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCACAAGAGTGGTGGTTTTTGTATATGAG29630.3353930822771413No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAG28370.32113066973346266No Hit
CTCTTGTGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTC26960.3051703509345842No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCC25680.29068155089021225No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG25460.28819128838258584No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCT22290.25230886952269593No Hit
CCTCAGAGGAGGGCGGGAGCAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC21370.24189504449080357No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCACAAGAGTGGTG20930.23691451947555073No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT20380.23068886320648466No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC17850.20205084436878074No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG17480.19786267560595447No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCAGTGGAACCAGCAGTGACGTT17300.19582518809971466No Hit
GTAATAGACGGCCGTGTCCGCGGCAGTCACAGAGTTCAGGTTCAGGGAGAACTGGTTCTTAGACGGGTCTAGTGA17090.19344811934243486No Hit
GGGTTTATTGAGTGGAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG16870.19095785683480845No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGCTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG15920.18020445055187612No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC15440.17477115053523665No Hit
GTCGATCACCATCTCCTGCAGTGGAACCAGCAGTGACGTTGGTTTTTATAACTATGTCTCCTGGTACCAACAACA14660.1659420380081975No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGCTCCCGCCCTC14510.16424413175299765No Hit
TCTCTGGGCTCCAACCTGACGACGAGGCTGATTATTATTGCAGCTCATATACAAAAACCACCACTCTTGTGGTGT14290.16175386924537122No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA14180.16050873799155801No Hit
GACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCCTGCAGTGGAACCAGCAGTGACGT14090.15948999423843813No Hit
GGTTTATTGAGTGGAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG13480.1525851754672921No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT13460.1523587879665988No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC13360.15122685046313225No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC13320.1507740754617456No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAGCAGAGTGACCGAGGGGGCAGCCTTGGG13240.14986852545897236No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG13090.14817061920377253No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGC13050.1477178442023859No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA12780.14466161294302618No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA12730.14409564419129292No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC12650.14319009418851966No Hit
CTCTGCTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC12520.14171857543401314No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCTGACGCCTGAGCAG12500.14149218793331983No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA12400.14036025042985328No Hit
GATTATTATTGCAGCTCATATACAAAAACCACCACTCTTGTGGTGTTCGG11910.13481375666286713No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATGGGTGGGTTTACCCGCCAAGCGG11680.13221030040489404No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGG11550.13073878165038752No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGT11420.12926726289588097No Hit
GGCCTGGGCTCTGCTGCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGC11360.12858810039380106No Hit
CTCCTGCAGTGGAACCAGCAGTGACGTTGGTTTTTATAACTATGTCTCCTGGTACCAACAACACCCAGGCAAAGC11190.1266638066379079No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT11130.12598464413582797No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA11100.12564506288478802No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACACCTGTGGTTCTTCCTCCTGCTGGTGGCAGCTCCCAGATGGGTC10890.12326799412750823No Hit
GTCTGAGAGTCCTGGACCTCCTGTGCAAGAACATGAAACACCTGTGGTTC10700.12111731287092176No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAGTTGAGGGAGATGGGGTAGGTGGAGT10650.1205513441191885No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC10630.12032495661849518No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT10580.1197589878667619No Hit
CTTATACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGT10460.11840066286260202No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT10420.1179478878612154No Hit
GTCTATTACTGTACGAGAGAAGATGGCAGTGGCTGGTACCTTGATGCTTTTGATCTCTGGGGCCAAGGAACAA10240.1159104003549756No Hit
GCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCCTGCAGTGGAACCAGCAG10160.11500485035220237No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG9790.11081668158937608No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCACAAGAGTGGTGG9750.11036390658798946No Hit
GACCATCTCTGGGCTCCAACCTGACGACGAGGCTGATTATTATTGCAGCTCATATACAAAAACCACCACTCTTG9650.10923196908452291No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG9600.10866600033278963No Hit
CTGCTGCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTG9550.10810003158105634No Hit
CTATGTCTCCTGGTACCAACAACACCCAGGCAAAGCCCCCAAACTCATGA9520.10776045033001637No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCACAAGAGTGG9490.10742086907897641No Hit
TATTTATTGAGGGTTTATTGAGTGGAGGGAGAAGGGCTTGATGCCTTGGG9310.10538338157273659No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCT9250.10470421907065666No Hit
CGGTCACTCTGCTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACT9240.10459102532031002No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT9220.10436463781961669No Hit
CCCCTGAGGGCCGATTTGTGACTTCATAAATCATGAGTTTGGGGGCTTTGCCTGGGTGTTGTTGGTACCAGGAGA9150.10357228156719012No Hit
GAGATGGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCAGAGAAGCGATC8980.10164798781129696No Hit
GCTTGGTCCCTCCGCCGAACACCACAAGAGTGGTGGTTTTTGTATATGAGCTGCAATAATAATCAGCCTCGTCG8980.10164798781129696No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG3400.095.3962870
GACCGGA500.00300621364.8694870
AGCTGCG650.0084915149.89959770
GTGGTAT1600.048.9454881
GGTATCA8500.045.2651821
TATCGCG704.838512E-1043.9640336
ATGGCAA1751.6502963E-743.24631570
AATGGTA2051.198714E-842.19152570
CGCGTAT504.0112845E-641.1407139
GTATCGC759.604264E-1040.99346535
ATCGCGT603.1033233E-739.9205337
TATCTCG2600.039.454936
GTATCAA21550.039.026041
TTATACA3800.038.516092
TGGTATC1950.038.401252
GGTAGAA17150.037.8247670
TATACAC4050.037.8236923
TCGTATG2450.037.81461740
ACCGATC3700.037.74283227
ACGAGAC3700.037.72787522
Produced by FastQC (version 0.11.5)