FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685197

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685197
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences883441
Sequences flagged as poor quality0
Sequence length18-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG40160.45458610139217No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGCTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGT34400.3893865011924962No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT27050.3061890946877041No Hit
CCACAAGAGTGGTGGTTTTTGTATATGAGCTGCAATAATAATCAGCCTCG26210.296680819658585No Hit
CTCTTGTGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCT26110.29554888215511843No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG25300.2863801883770393No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCCT25130.2844558946211462No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCACAAGAGTGGTGGTTTTTGTATATGA25020.283210763367333No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT21300.241102688238377No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC19480.22050142567528563No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT19170.21699241941453926No Hit
CCTCAGAGGAGGGCGGGAGCAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC19040.21552090066003274No Hit
GGGTTTATTGAGTGGAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCA18610.21065356939512656No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATA17300.19582518809971466No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCACAAGAGTGGTG17170.19435366934520812No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCAGTGGAACCAGCAGTGACGTT16220.1836002630622758No Hit
TCTCTGGGCTCCAACCTGACGACGAGGCTGATTATTATTGCAGCTCATAT16200.1833738755615825No Hit
GTAATAGACGGCCGTGTCCGCGGCAGTCACAGAGTTCAGGTTCAGGGAGAACTGGTTCTTAGACGGGTCTAGT15920.18020445055187612No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGCTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAG14710.16650800675993077No Hit
GTCGATCACCATCTCCTGCAGTGGAACCAGCAGTGACGTTGGTTTTTATAACTATGTCTCCTGGTACCAACAACA13940.15779208798323827No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC13810.15632056922873172No Hit
GACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCT13730.1554150192259585No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTT13150.14884978170585247No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCT13000.1471518754506526No Hit
GGTTTATTGAGTGGAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCA12970.14681229419961267No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTT12750.1443220316919862No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGCTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAG12490.14137899418297317No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTG12320.13945470042708003No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA12040.13628527541737365No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACG11830.1339082066600939No Hit
CTCTGCTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC11680.13221030040489404No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC11470.12983323164761426No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC11340.12836171289310774No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAGCAGAGTGACCGAGGGGGCAGCCTTGG11110.12575825663513465No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAGCAGAGTGACCGAGGGG11030.12485270663236141No Hit
GTCTGAGAGTCCTGGACCTCCTGTGCAAGAACATGAAACACCTGTGGTTCTTCCTCCTGCTGGTGGCAGCTCCCA11020.12473951288201476No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGC10940.1238339628792415No Hit
GATTATTATTGCAGCTCATATACAAAAACCACCACTCTTGTGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTA10890.12326799412750823No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT10840.12270202537577495No Hit
GCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCCTGCAGTGGAACCAGCAGT10760.1217964753730017No Hit
CTGCTGCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTC10550.11941940661572192No Hit
GGCCTGGGCTCTGCTGCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGC10490.118740244113642No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTG10470.11851385661294869No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG10280.11636317535636223No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACACCTGTGGTTCTTCCTCCTGCTGGTGGCAGCTCCCAGATGGGTC10260.11613678785566892No Hit
CTCCTGCAGTGGAACCAGCAGTGACGTTGGTTTTTATAACTATGTCTCCTGGTACCAACAACACCCAGGCAAAGC10220.1156840128542823No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT10060.1138729128487358No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCA10030.11353333159769582No Hit
GACCATCTCTGGGCTCCAACCTGACGACGAGGCTGATTATTATTGCAGCTCATATACAAAAACCACCACTCTTGT9940.11251458784457592No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC9800.11092987533972275No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTT9690.10968474408590953No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTC9460.10708128782793644No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTTCCATGGCTCGGCACAGCCCGGCAG9390.10628893157550985No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC9330.10560976907342992No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC9330.10560976907342992No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGACTTGCCGGCTAGGCACTGTGTGGCC9280.10504380032169663No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCACAAGAGTGGTGG9260.10481741282100333No Hit
CTATGTCTCCTGGTACCAACAACACCCAGGCAAAGCCCCCAAACTCATGA9240.10459102532031002No Hit
GTGGAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC9190.10402505656857673No Hit
AGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCCTGCAGTGGAACCA9160.10368547531753676No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG9140.10345908781684346No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC9060.1025535378140702No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAG9050.10244034406372356No Hit
CCCCTGAGGGCCGATTTGTGACTTCATAAATCATGAGTTTGGGGGCTTTGCCTGGGTGTTGTTGGTACCAGGA8960.10142160031060365No Hit
GTCTATTACTGTACGAGAGAAGATGGCAGTGGCTGGTACCTTGATGCTTTTGATCTCTGGGGCCAAGGAACAATG8900.10074243780852372No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATTCTA851.2148976E-680.719370
AATGGTA2500.065.8669570
CAAGGTA4250.058.1178970
ATCCGTT1853.9099177E-851.92214270
GGTAGAA17200.050.2618470
GTGGTAT1750.048.6082651
AAAGTCA1701.3004701E-648.4315870
TTCAGTA1155.2521634E-447.72966870
GGTATCA7250.045.5241551
GTATCAA22400.042.9879341
TTAACGG405.2937285E-542.7113235
AGATGGT22000.042.4143270
TCAAGGT5850.039.87671370
TGGTATC2150.039.5335042
ATGGCAA1758.7481065E-539.20651670
CGCAATC459.059417E-538.99063562
AACCGTG459.674856E-538.5580154
TAACCGT459.744217E-538.51127253
CGGTACC451.0097221E-438.27927445
TATCAAC25500.037.7320142
Produced by FastQC (version 0.11.5)