FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004685198

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685198
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1067413
Sequences flagged as poor quality0
Sequence length18-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACAGGGGACTGGCCTGGCCTCTGCTGATACCAGCAAGCAGATTTATGCCCCAGTTTATCTCCAGAGCAGTTGA32850.3077534187797975No Hit
CCCCTGTACTGATCATCTATGAACATGTCAAGCGGCCCTCAGGGATCCCTGAGCGATTCTCTGGCTCCAAATC32430.30381867187302386No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG32280.30241340512060466No Hit
GTAATAAATAGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATACA29400.27543228347415666No Hit
GTATATGTTAGTACTACTACTGGTAATACATGAAAGCCATTCCAGCCCCTTCCCTGGAGCCTGGCGGACCCAAT28570.26765647411077065No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGG25660.2403942991138388No Hit
AACATATACTATGCAGACTCTGTGAAGGGCCGATTCACCGTCTCCAGAGACAACACCAAGAAGTCACTGTATC23470.21987740452851895No Hit
CCTATGAGCTGACTCAGCCACCCTCAGTGTCCGTGTCCCCAGGACAGACA23180.2171605554738419No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG19280.18062361991094356No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAAACAGCCGTGCCGCTGTCCCACGCCTGACAA19190.17978045985949206No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA19050.17846887755723417No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAG18940.17743834860546012No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC18890.17696992635465372No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG18790.17603308185304095No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT18770.1758457129527184No Hit
GGATTTGTGGCCTCCTATGAGCTGACTCAGCCACCCTCAGTGTCCGTGTCCCCAGGACAGACAGCCAGCATCAA17880.16750779688836467No Hit
ATTTATTACTGTGTGAGAGATGGGGGGGGTTGGTACTTCGATCTCTGGGGCCGTGGCACCCTGGTCACTGTCTC17600.1648846322838489No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACTTCGCCCACAGCATCCTGACCGTGTC17200.16113725427739778No Hit
CTTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGT16890.15823303632239819No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT16420.1538298671648181No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC16250.1522372315120764No Hit
TCTATGAACATGTCAAGCGGCCCTCAGGGATCCCTGAGCGATTCTCTGGCTCCAAATCTGGGAACACAGCCAC16250.1522372315120764No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCT15650.14661616450239973No Hit
GGCCAGGCCAGTCCCCTGTACTGATCATCTATGAACATGTCAAGCGGCCCTCAGGGATCCCTGAGCGATTCTC15290.14324352429659373No Hit
GTCCTGAGAGGGGGCAAGTACGCAGCCACCTCACAGGTGCTGCTGCCTTC14900.1395898307403039No Hit
GAGTTGGGGCTGTGCTGGGTTTTCCTTGTTGCTATTTTAGAAGGTGTCCAGTGTGAGGTGCAACTGGTGGAG14760.13827824843804598No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAAACAGCCGTGCCGCTGTCCCACGCCTGACAATAATATTCAG14750.13818456398788473No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT14250.13350034147982084No Hit
GTACAGGGTGGGTTTACCGGTGGACTTGTCCACGGTCCTCTCGGTGACCCTGTTGGGCAGGGCCTCATGGGCCAC13780.1290971723222408No Hit
CTCCTGGACCCGCCAGAATGGCGAAGCTGTGAAAACCCACACCAACATCTCCGAGAGCCACCCCAATGCCACTT13680.12816032782062803No Hit
CCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGTG13240.12403821201353178No Hit
GCACTGAACACAGAGGACTCACCATGGAGTTGGGGCTGTGCTGGGTTTTC12750.11944767395562918No Hit
CCCTCAGTGTCCGTGTCCCCAGGACAGACAGCCAGCATCAACTGCTCTGGAGATAAACTGGGGCATAAATCTGCT12580.11785503830288745No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG12580.11785503830288745No Hit
GTACTGATCATCTATGAACATGTCAAGCGGCCCTCAGGGATCCCTGAGCG12420.11635608710030701No Hit
GGATGGGGGGATGGCGAAGACCCGGATGGCTGTGTCTTGATCGGGGACAC12420.11635608710030701No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG12260.11485713589772656No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC12170.11401397584627505No Hit
GTACCGGCCTGGGGCCTGGGGCTCAGGCATTGGGGCGCTGGTCACATACTTCTCCGGGGACAAGGGCTGCCCCC12150.11382660694595251No Hit
CTCCATGTGTGTCCCCGATCAAGACACAGCCATCCGGGTCTTCGCCATCCCCCCATCCTTTGCCAGCATCTTCC12070.11307713134466228No Hit
CTCCTGTGCAGCCTCTGGATTCAGCTTCAGTAATTATGAAATGAATTGGGTCCGCCAGGCTCCAGGGAAGGGG11950.11195291794272695No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC11920.1116718645922431No Hit
GGTCACATACTTCTCCGGGGACAAGGGCTGCCCCCTCTGCATCCACTGCACGAAGACGTCCGCGGGAGAGAAGCC11750.1100792289395014No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG11570.1083929088365984No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC11430.1070813265343405No Hit
GTCCAGCACCCCAACGGCAACAAAGAAAAGAACGTGCCTCTTCCAGTGAT11280.10567605978192134No Hit
TCTCAGGACTGATGGGAAGCCCCGGGTGCTGCTGATGTCAGAGTTGTTCT11140.10436447747966345No Hit
GTGCTGGGTTTTCCTTGTTGCTATTTTAGAAGGTGTCCAGTGTGAGGTGCAACTGGTGGAGTCTGGGGGAGGCT10990.10295921072724429No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC10980.10286552627708301No Hit
GACCTGCCCTCGCCACTGAAGCAGACCATCTCCCGGCCCAAGGGGGTGGCCCTGCACAGGCCCGATGTCTACT10850.1016476284249864No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGG10820.10136657507450256No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG10800.10117920617418001No Hit
GTCCCACGCCTGACAATAATATTCAGCCTCATCCATAGTCTGGGTCCCGC10710.1003360461227285No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)