FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685200

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685200
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1055037
Sequences flagged as poor quality0
Sequence length19-76
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATATAGGGCCGTGTCGTCGGCTCTCAGGCTGTTCATTTGCAGATACA54440.5160008606333237No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCCGGGTCCCTCCGCCGAATAGGAAAGTGTTCCTACCTGCATATGAGCAG32960.31240610518872797No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGG32090.30415994889278763No Hit
CTATATTACTGTGCGAAAGATCTGGATTATGATCTTTGGAGGGGGGCTTTTGATATTTGGGGCCAAGGGACAA30080.2851084843469945No Hit
GGACTGAACAGAGAGAACTCACCATGGAATTTGGGCTGAGCTGGCTTTTT28820.2731657752287361No Hit
GATTATTACTGCTGCTCATATGCAGGTAGGAACACTTTCCTATTCGGCGGAGGGACCCGGCTGACCGTCCTAGG28770.2726918582002337No Hit
ATATTACTGTGCGAAAGATCTGGATTATGATCTTTGGAGGGGGGCTTTTGATATTTGGGGCCAAGGGACAATGG26560.25174472554043126No Hit
CAGTAATATAGGGCCGTGTCGTCGGCTCTCAGGCTGTTCATTTGCAGATACAGTGTGTTCTTGGGATTGTCTC25830.24482553692429745No Hit
GACCTAGGACGGTCAGCCGGGTCCCTCCGCCGAATAGGAAAGTGTTCCTACCTGCATATGAGCAGCAGTAATAA25620.2428350854045877No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG24830.23534719635425108No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA23130.21923401738517226No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAGTTGAGGGAGATGGGGTAGGTGGAGT22370.21203047855193705No Hit
GTACCAACACCACCCAGGCAAAGCCCCCAAACTCATGATTTATGAGGGCAGTAAGCGGCCCTCAGGGGTTTCTAA22220.21060872746643008No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCC22150.20994524362652686No Hit
GTAATAATCAGCCCCGTCCTCAGCCTGGAGCCCAGAGATTGTCAGGGAGG21660.20530085674720414No Hit
CTCCTGTGCAGCCTCTGGATTCCCCTTTAGCAACTATGCCATGACCTGCGTCCGCCAGGCTCCAGGGAAGGGG21060.19961385240517632No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCACTGGAACCAGCAGTGATGTT20880.19790775110256797No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAG20500.19430598168595034No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT20200.19146247951493645No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG19580.1855859083615077No Hit
GTCTGGGGGAGGCTTGGTACAGCCGGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCCCCTTTAGCAA19090.18094152148218498No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCCGGGTCCCTC19070.18075195467078406No Hit
ATATAGGGCCGTGTCGTCGGCTCTCAGGCTGTTCATTTGCAGATACAGTGTGTTCTTGGGATTGTCTCTGGAGA19030.1803728210479822No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG19010.18018325423658127No Hit
GCCCTATATTACTGTGCGAAAGATCTGGATTATGATCTTTGGAGGGGGGC18560.1759180009800604No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC18240.1728849319976456No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT18110.17165274772353956No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC18110.17165274772353956No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTT18000.17061013026083446No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT17890.16956751279812934No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC17700.16776662808982054No Hit
CTCCTGCACTGGAACCAGCAGTGATGTTGGGAGTTTTAACCTTGTCTCCTGGTACCAACACCACCCAGGCAAAGC17520.1660605267872122No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAGTGTCCTGCCGGGCTGTGCCGAGCCA17370.16463877570170526No Hit
GTGTTGGTACCAGGAGACAAGGTTAAAACTCCCAACATCACTGCTGGTTCCAGTGCAGGAGATGGTGATCGA17320.16416485867320293No Hit
GGACACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC17170.16274310758769597No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG16560.1569613198399677No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGG16270.15421260107465426No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC16240.15392825085755285No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC15710.14890473035542828No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA15420.14615601159011485No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGC15350.1454925277502116No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC15340.14539774434451114No Hit
GTCGATCACCATCTCCTGCACTGGAACCAGCAGTGATGTTGGGAGTTTTAACCTTGTCTCCTGGTACCAACACCA15300.1450186107217093No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG15290.1449238273160088No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATGGGTGGGTTTACCCGCCAAGCGG15250.14454469369320697No Hit
GACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCCTGCACTGGAACCAGCAGTGATGT15130.1434072928248014No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT15100.1431229426077No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCC14680.13914203956828056No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC14480.13724637145427127No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA14460.13705680464287034No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT14360.1361089705858657No Hit
GTATGTGCTACCGCTACCGCTAATAGTTGAGACCCACTCCAGCCCCTTCCCTGGAGCCTGGCGGACGCAGGTCA14350.13601418718016525No Hit
GAACAGAGAGAACTCACCATGGAATTTGGGCTGAGCTGGCTTTTTCTTGTGGCTATTTTAAAAGGTGTCCAGTG14180.13440286928325737No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG14170.1343080858775569No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT14110.13373938544335412No Hit
GTATCAACGCAGAGTACGGGAGCTCTGAGAGAGGAGCCCAGCCCTGGGAT13920.13193850073504532No Hit
CCTTGGGGCTGGTCGGGGATGCTGAAGAGACGGTGACCATTGTCCCTTGG13900.1317489339236444No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAGGTCCCCGGAGGCATCCTGGCTGGG13800.13080109986663974No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG13650.1293793487811328No Hit
GATCAGGACTGAACAGAGAGAACTCACCATGGAATTTGGGCTGAGCTGGCTTTTTCTTGTGGCTATTTTAAAAGG13530.12824194791272722No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCCGGTCGGTGCAGTGACAGTCGGGGG13510.1280523811013263No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA13300.12606192958161658No Hit
GGCCTGGGCTCTGCTGCTCCTCACTCTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGC13290.12596714617591612No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGC13220.12530366233601287No Hit
GTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGG13060.12378712784480546No Hit
AGCACATACTACGCAGACTCCGTGAGGGGCCGGTTCACCATCTCCAGAGACAATCCCAAGAACACACTGTATC12940.12264972697639989No Hit
GGACAATGGTCACCGTCTCTTCAGCATCCCCGACCAGCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGC12790.12122797589089293No Hit
CAGTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCC12730.12065927545669014No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGT12590.11933230777688365No Hit
TCCTTGGGGCTGAAGCCGCGTGCCAGGCACGTCAGCGTCACCAGCTCGTT12530.11876360734268086No Hit
CTCCAGCCCCTTCCCTGGAGCCTGGCGGACGCAGGTCATGGCATAGTTGC12480.11828969031417856No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC12390.11743663966287438No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGG12360.11715228944577298No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG12340.11696272263437206No Hit
GGGCTGGAGTGGGTCTCAACTATTAGCGGTAGCGGTAGCACATACTACGC12290.11648880560586974No Hit
GTCACGGACTTGCCGGCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCT12010.11383487024625677No Hit
GAGTACGGGAGCTCTGAGAGAGGAGCCCAGCCCTGGGATTTTCAGGTGTT11870.11250790256645028No Hit
GTTTTAACCTTGTCTCCTGGTACCAACACCACCCAGGCAAAGCCCCCAAA11840.11222355234934889No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGG11760.11146528510374518No Hit
CCATTGTCCCTTGGCCCCAAATATCAAAAGCCCCCCTCCAAAGATCATAA11730.11118093488664378No Hit
ACCTAGGACGGTCAGCCGGGTCCCTCCGCCGAATAGGAAAGTGTTCCTAC11680.11070701785814148No Hit
GCCCTGGGATTTTCAGGTGTTTTCATTTGGTGATCAGGACTGAACAGAGA11640.11032788423533961No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC11620.11013831742393869No Hit
GTGTGAGGTGCAGCTGGTGCAGTCTGGGGGAGGCTTGGTACAGCCGGGGGGGTCCCTGAGACTCTCCTGTGCAGC11520.10919048336693404No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA11460.10862178293273128No Hit
GCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCCTGCACTGGAACCAGCAG11440.10843221612133033No Hit
CCCTGAGACTCTCCTGTGCAGCCTCTGGATTCCCCTTTAGCAACTATGCC11270.10682089822442245No Hit
CTGCAGCACCCAGCCAGATGGGAACGTGGTCATCGCCTGCCTGGTCCAGGGCTTCTTCCCCCAGGAGCCACTCAG11150.10568349735601688No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC11100.1052095803275146No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCTGACGCCTGAGCAG11100.1052095803275146No Hit
CTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCC10920.10350347902490624No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT10890.10321912880780484No Hit
GTGTAGTGCTTCACGTGGCATGTCACGGACTTGCCGGCTAGGCACTGTGT10830.10265042837360207No Hit
GTGCTGCAGAGGCTCAGCGGGAAGACCTTGGGGCTGGTCGGGGATGCTGAAGAGACGGTGACCATTGTCCCT10780.10217651134509975No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC10580.10028084323109047No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGGAG19200.062.21552370
CGTACGC200.006745555452.9013865
CGCATTT200.007698203451.1632522
AATGGTA1251.0663061E-649.8589870
GGTATCA6150.048.262961
GTGGTAT1350.045.4892231
CGAAACC604.0101146E-544.76699469
TCGTATG1200.042.8840940
TCTCGTA1200.042.84118738
CTCTTAT504.148982E-640.9403041
GTATCAA16350.040.481241
ATGCCGT1200.040.11506344
CGTATGC1200.040.0480741
GTGGTGA1307.83967E-539.95110370
AGTAAGA1050.001322920939.57061870
TCTACAT1300.039.41427232
TGGTATC1500.038.642022
ATCTCGT1350.038.0520537
TATACAC1350.037.8861273
GCCGTCT1300.037.07890746
Produced by FastQC (version 0.11.5)