FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685204

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685204
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences930513
Sequences flagged as poor quality0
Sequence length18-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAAAGAGCCGTGTCGTCAATTGTCAGGCTGCTCATTTGCAGAAACA44940.4829593998149408No Hit
CTCTAGGCCCTTGCCTGGAGCCTGGCGGACCCAGTGCATAGAATAATCAC32170.34572327307624934No Hit
CTTTATTACTGTGCGAGAGATTGGAGTCCCTTCTCTCTTTGGAATCCTGTTGACTATTGGGGCCAGGGAACCC32060.34454112946299514No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTAT27180.2920969400749909No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC25210.27092582263762033No Hit
CCCTAAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCAGTGATTATTCT24110.2591043865050784No Hit
GGCCTAGAGTGGGTGGCAGTTATATCGTATGATGGCCTTAGAGAATATTG21530.23137774539420727No Hit
GTCCTGCAGGGCCAGTGAAAGTCTTAGCAGCACCTACCTAGCCTGGTACC20470.21998617966648504No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA20340.21858910085082098No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAGTTGAGGGAGATGGGGTAGGTGGAGT19180.20612285911104947No Hit
CTCCATTAGCAGCCTGGAGCCTGAAGATTTTGCAGTTTATTACTGTCAACAATTTGGTAACTCGCCTGTGGCGT18970.20386603948574605No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT18880.2028988310749017No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCT18260.19623583980019627No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG17670.18989525132910556No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG16710.17957836161343257No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC16090.17291537033872714No Hit
GCACTGAACACAGAGGACTCACCATGGAGTTTGGGCTGAGCTGGGTTTTC16040.1723780323327025No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC15700.168724133891735No Hit
GTCTTAGGGACCTCCCAGGCTGGACCACGCCTCCCCCAGACTCCACCAGC15290.164317962242333No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC15280.16421049464112805No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC15220.1635656890338985No Hit
GCACTGGAAGTCGCCGGTGTTTCCATTCGGTGATCAGCACTGAACACAGA15030.16152380461100488No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAGTGTCCTGCCGGGCTGTGCCGAGCCA14930.16044912859895563No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGG14900.16012672579534087No Hit
CATCTATGCTGCATCTACCAGGGCCACTGGCATCCCAGACAGGTTCCGGGGCCTTGGGTCTGGGACAGACTTCA14580.15668776255678318No Hit
GTTTATTACTGTCAACAATTTGGTAACTCGCCTGTGGCGTTCGGCCAAGGGACCAAGGTGGAAATGAAACGAA14400.1547533457350945No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG14270.15335626691943047No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT14180.1523890585085861No Hit
GCTAATGGAGAGAGTGAAGTCTGTCCCAGACCCAAGGCCCCGGAACCTGTCTGGGATGCCAGTGGCCCTGGTAGA14110.15163678530015165No Hit
GAGTGAAGTCTGTCCCAGACCCAAGGCCCCGGAACCTGTCTGGGATGCCAGTGGCCCTGGTAGATGCAGCATAGA14060.151099447294127No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG13950.1499173036808728No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA13910.1494874332760531No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATGGGTGGGTTTACCCGCCAAGCGG13870.1490575628712334No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC13760.1478754192579792No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCC13740.14766048405556934No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT13730.14755301645436442No Hit
GACTTCACTCTCTCCATTAGCAGCCTGGAGCCTGAAGATTTTGCAGTTTATTACTGTCAACAATTTGGTAACTCG13730.14755301645436442No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT13630.14647834044231514No Hit
GTAATAAACTGCAAAATCTTCAGGCTCCAGGCTGCTAATGGAGAGAGTGA13570.1458335348350856No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG13400.14400658561460183No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAAC13060.14035268717363433No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT12880.13841827035194565No Hit
GGGTTCCCTGGCCCCAATAGTCAACAGGATTCCAAAGAGAGAAGGGACTCCAATCTCTCGCACAGTAATAAAGAG12660.13605398312543726No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA12570.13508677471459293No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCC12560.134979307113388No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTCAGTGATTATTCTATGCACTGGG12480.13411956630374858No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC12460.13390463110133874No Hit
ATATTGGGCAGAGTCCGTGAAGGGCCGATTCACCATCTCCAGAGACAACTCCAAGAACACGTTGTTTCTGCAAA12390.13315235789290422No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAGGTCCCCGGAGGCATCCTGGCTGGG12000.1289611214459121No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT11830.12713417222542833No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCCGGTCGGTGCAGTGACAGTCGGGGG11750.12627443141578892No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC11670.12541469060614951No Hit
GTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGG11640.12509228780253473No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTCATTTCCACCTTGGTCC11500.12358774138566575No Hit
GAGTTTGGGCTGAGCTGGGTTTTCCTCGCTGTTCTTTTAAGAGGTGTCCAGTGTCAGGTACAGCTGGTGGAG11480.1233728061832559No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG11270.12111598655795243No Hit
TCCTTGGGGCTGAAGCCGCGTGCCAGGCACGTCAGCGTCACCAGCTCGTT11230.12068611615313274No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACT11220.12057864855192782No Hit
GTGCTGCAGAGGCTCAGCGGGAAGACCTTGGGGCTGGTCGGGGATGCTGAGGAGACGGTGACCAGGGTTCCC11020.11842929652782928No Hit
CAGTAATAAAGAGCCGTGTCGTCAATTGTCAGGCTGCTCATTTGCAGAAACAACGTGTTCTTGGAGTTGTCTC10800.1160650093013209No Hit
GTCCCAGACCCAAGGCCCCGGAACCTGTCTGGGATGCCAGTGGCCCTGGTAGATGCAGCATAGATGAGGAGCC10710.11509780089047654No Hit
GATCAGCACTGAACACAGAGGACTCACCATGGAGTTTGGGCTGAGCTGGGTTTTCCTCGCTGTTCTTTTAAGAGG10700.1149903332892716No Hit
CATTGACATGGGTGGGTTTACCCGCCAAGCGGTCGATGGTCTTCTGTGTGAAGGCCAGCGGCAGGGCCTCGTGGC10690.1148828656880667No Hit
GCATAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTGTGCTGGTACCAGGCTAGGTAGGTGCTGCTAAGACTTTCA10680.11477539808686177No Hit
GTATCAACGCAGAGTACGGGAGCTCTGGGAGAGGAGCCCAGCACTGGAAG10590.11380818967601741No Hit
GTGCAGCCACAGTTCGTTTCATTTCCACCTTGGTCCCTTGGCCGAACGCC10570.11359325447360757No Hit
CAGTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCC10570.11359325447360757No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGTGAAAGAGCCACCCTGTCCTGCAGGGCCAGTGAAAGTCTTAGCAG10490.11273351366396815No Hit
GTACCTGACTTGGGCATCCCGGCAGGAGCCCAGCCAGGGCACCACCACCTTCGCTGTGACCAGCATACTGCGCG10470.11251857846155829No Hit
CCTTGGGGCTGGTCGGGGATGCTGAGGAGACGGTGACCAGGGTTCCCTGG10400.11176630525312381No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT10330.11101403204468933No Hit
ATATAACTGCCACCCACTCTAGGCCCTTGCCTGGAGCCTGGCGGACCCAG10250.1101542912350499No Hit
GTCTTTGTCTCCAGGTGAAAGAGCCACCCTGTCCTGCAGGGCCAGTGAAAGTCTTAGCAGCACCTACCTAGCC10190.10950948562782035No Hit
GGTTTGTGCTGGTACCAGGCTAGGTAGGTGCTGCTAAGACTTTCACTGGCCCTGCAGGACAGGGTGGCTCTTTCA10130.1088646800205908No Hit
GCCCAGCACTGGAAGTCGCCGGTGTTTCCATTCGGTGATCAGCACTGAAC10080.10832734201456616No Hit
GTCACGGACTTGCCGGCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCT10030.10779000400854151No Hit
CAGTTAGGACGCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCCTCCTCCTGCTACTCTGGCTCCCAGATAC9940.10682279559769718No Hit
ACCCTGTCCTGCAGGGCCAGTGAAAGTCTTAGCAGCACCTACCTAGCCTGGTACCAGCACAAACCTGGCCAGGC9910.10650039279408241No Hit
GTTAGGACGCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCCTCCTCCTGCTACTCTGGCTCCCAGATACCA9830.10564065198444299No Hit
GTGTAGTGCTTCACGTGGCATGTCACGGACTTGCCGGCTAGGCACTGTGT9790.1052107815796233No Hit
GTGTCAGGTACAGCTGGTGGAGTCTGGGGGAGGCGTGGTCCAGCCTGGGAGGTCCCTAAGACTCTCCTGTGCAGC9760.10488837877600851No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT9620.10338383235913952No Hit
GAGTACGGGAGCTCTGGGAGAGGAGCCCAGCACTGGAAGTCGCCGGTGTTTCCATTCGGTGATCAGCACTGAACA9600.10316889715672967No Hit
CAGTTATATCGTATGATGGCCTTAGAGAATATTGGGCAGAGTCCGTGAAG9560.10273902675190998No Hit
CTCCAGAGACAACTCCAAGAACACGTTGTTTCTGCAAATGAGCAGCCTGACAATTGACGACACGGCTCTTTATTA9500.1020942211446804No Hit
GCACAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGCTGCATCTACCAGGGCCACTGGCATCCCAGACAGGT9400.10101954513263113No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT9330.10026727192419665No Hit
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGT9310.1000523367217868No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCACAG11300.069.97356470
TCTGGAG13000.068.33221470
CTCTTAA8150.051.50368570
GGTATCA5850.048.2658121
TTCTTAA856.347018E-445.93762270
TGTTAGA6750.043.3855370
ACTAGAT1650.041.34586732
CTGCACA11450.039.6947169
ACCGTAC1750.038.9266127
GACCGTA1750.038.9119526
GCGCTTT350.00143794638.9119526
GAGACCG1800.037.8147924
GTATCAA14650.037.385711
CTAGATC1650.037.22733333
ATGCCGT1850.037.17656744
CGTATGC1850.037.0879141
GTACTAG1850.036.85022730
AGACCGT1850.036.79672225
CGTACTA1900.035.86503229
ATCTCGT1750.035.17401537
Produced by FastQC (version 0.11.5)