FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004685205

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685205
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences930513
Sequences flagged as poor quality0
Sequence length18-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAAAGAGCCGTGTCGTCAATTGTCAGGCTGCTCATTTGCAGAAACAACGTGTTCTTGGAGTTGTCTCTGGA38770.416651889871501No Hit
CTTTATTACTGTGCGAGAGATTGGAGTCCCTTCTCTCTTTGGAATCCTGTTGACTATTGGGGCCAGGGAACCCTG29990.3222953360135753No Hit
CTCTAGGCCCTTGCCTGGAGCCTGGCGGACCCAGTGCATAGAATAATCACTGAAGGTGAATCCAGAGGCTGCACA29050.3121933815003122No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT26320.28285472637136716No Hit
GGCCTAGAGTGGGTGGCAGTTATATCGTATGATGGCCTTAGAGAATATTG24250.26060893292194737No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG24190.2599641273147178No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTA23770.25545048806411086No Hit
CCCTAAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCAGTGATTATTCTATGCACTGGGTCCGCCAGGCTCCAG20350.21869656845202595No Hit
GTCCTGCAGGGCCAGTGAAAGTCTTAGCAGCACCTACCTAGCCTGGTACCAGCACAAACCTGGCCAGGCTCCCAG18210.1956985017941716No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG17300.18591895008452328No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGACTTGCCGGCTAGGCACTGTGTGGCC17080.1835546628580149No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTT17030.18301732485199024No Hit
CTCCATTAGCAGCCTGGAGCCTGAAGATTTTGCAGTTTATTACTGTCAACAATTTGGTAACTCGCCTGTGGCGTT16970.1823725192447607No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC16790.18043810242307198No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAG16230.17441991675559612No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTTCCATGGCTCGGCACAGCCCGGCAG16210.17420498155318626No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGC15750.1692614718977596No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC15720.16893906909414483No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG15190.16324328623028372No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTC15140.16270594822425907No Hit
GCACTGAACACAGAGGACTCACCATGGAGTTTGGGCTGAGCTGGGTTTTC14800.15905204978329157No Hit
GACTTCACTCTCTCCATTAGCAGCCTGGAGCCTGAAGATTTTGCAGTTTATTACTGTCAACAATTTGGTAACTC14280.1534637345206354No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG14030.1507770444905122No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT13730.14755301645436442No Hit
GAGTGAAGTCTGTCCCAGACCCAAGGCCCCGGAACCTGTCTGGGATGCCAGTGGCCCTGGTAGATGCAGCATA13630.14647834044231514No Hit
GTCTTAGGGACCTCCCAGGCTGGACCACGCCTCCCCCAGACTCCACCAGC13520.14529619682906095No Hit
GCACTGGAAGTCGCCGGTGTTTCCATTCGGTGATCAGCACTGAACACAGAGGACTCACCATGGAGTTTGGGCTGA13470.14475885882303632No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA13460.1446513912218314No Hit
GTTTATTACTGTCAACAATTTGGTAACTCGCCTGTGGCGTTCGGCCAAGGGACCAAGGTGGAAATGAAACGAA13180.14164229838809345No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCC13060.14035268717363433No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC13040.14013775197122447No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAACGTGGTCATCGCCTGCCTGGTCCAGG13030.14003028437001955No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG12930.13895560835797027No Hit
GCTAATGGAGAGAGTGAAGTCTGTCCCAGACCCAAGGCCCCGGAACCTGTCTGGGATGCCAGTGGCCCTGGTA12530.1346569043097732No Hit
CATCTATGCTGCATCTACCAGGGCCACTGGCATCCCAGACAGGTTCCGGGGCCTTGGGTCTGGGACAGACTTCA12430.13358222829772395No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAA12430.13358222829772395No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGTTA12210.13121794107121557No Hit
GTAATAAACTGCAAAATCTTCAGGCTCCAGGCTGCTAATGGAGAGAGTGAAGTCTGTCCCAGACCCAAGGCCCCG12200.13111047347001062No Hit
GGGTTCCCTGGCCCCAATAGTCAACAGGATTCCAAAGAGAGAAGGGACTCCAATCTCTCGCACAGTAATAAAGA12140.13046566786278105No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT11890.1277789778326579No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT11890.1277789778326579No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGGGATGCCCAAGTCAGGTACTTCTCGC11760.12638189901699384No Hit
ATATTGGGCAGAGTCCGTGAAGGGCCGATTCACCATCTCCAGAGACAACTCCAAGAACACGTTGTTTCTGCAAAT11730.12605949621337906No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACT11680.12552215820735443No Hit
CCTTGGGGCTGGTCGGGGATGCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAATAGTCAACAGGATTCCAAA11530.12391014418928054No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC11270.12111598655795243No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCT11060.11885916693264897No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGCAGAG11010.11832182892662435No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT10970.11789195852180465No Hit
GAGTTTGGGCTGAGCTGGGTTTTCCTCGCTGTTCTTTTAAGAGGTGTCCAGTGTCAGGTACAGCTGGTGGAGT10890.11703221771216524No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC10880.1169247501109603No Hit
GTCCAAGACCCCGCTAACCGCCACCCTCTCAAAATCCGGAAACACATTCCGGCCCGAGGTCCACCTGCTGCCGCC10850.11660234730734552No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGC10760.11563513889650118No Hit
GTCTTTGTCTCCAGGTGAAAGAGCCACCCTGTCCTGCAGGGCCAGTGAAAGTCTTAGCAGCACCTACCTAGCCTG10740.11542020369409133No Hit
CAGTAATAAAGAGCCGTGTCGTCAATTGTCAGGCTGCTCATTTGCAGAAACAACGTGTTCTTGGAGTTGTCTCTG10630.11423806008083713No Hit
GTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGT10570.11359325447360757No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTCAGTGATTATTCTATGCACTGGG10390.11165883765191888No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT10380.11155137005071396No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGA10320.1109065644434844No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTCATTTCCACCTTGGTCC10310.11079909684227948No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCCGGTCGGTGCAGTGACAGTCGGGGGT10300.11069162924107455No Hit
GTGCAGCCACAGTTCGTTTCATTTCCACCTTGGTCCCTTGGCCGAACGCC10170.10929455042541049No Hit
GTATCAACGCAGAGTACGGGAGCTCTGGGAGAGGAGCCCAGCACTGGAAG10090.1084348096157711No Hit
GGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGACCTGTACACCACGAGCAGCCAGCTGAC9980.1072526660025169No Hit
CAGTTAGGACGCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCCTCCTCCTGCTACTCTGGCTCCCAGATAC9920.10660786039528732No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGCAGAGGCTCAGCGGGAAGACCTTGGG9910.10650039279408241No Hit
GATCAGCACTGAACACAGAGGACTCACCATGGAGTTTGGGCTGAGCTGGG9850.10585558718685284No Hit
GTCCCAGACCCAAGGCCCCGGAACCTGTCTGGGATGCCAGTGGCCCTGGTAGATGCAGCATAGATGAGGAGCCTG9810.10542571678203312No Hit
GAGTACGGGAGCTCTGGGAGAGGAGCCCAGCACTGGAAGTCGCCGGTGTTTCCATTCGGTGATCAGCACTGAACA9740.10467344357359865No Hit
GGTTTGTGCTGGTACCAGGCTAGGTAGGTGCTGCTAAGACTTTCACTGGCCCTGCAGGACAGGGTGGCTCTTTCA9710.10435104076998386No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAGGTCCCCGGAGGCATCCTGGCTGGGT9690.10413610556757402No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGTGAAAGAGCCACCCTGTCCTGCAGGGCCAGTGAAAGTCTTAGCA9690.10413610556757402No Hit
CCTGTACACCACGAGCAGCCAGCTGACCCTGCCGGCCACACAGTGCCTAGCCGGCAAGTCCGTGACATGCCACGT9670.10392117036516417No Hit
GTACCTGACTTGGGCATCCCGGCAGGAGCCCAGCCAGGGCACCACCACCTTCGCTGTGACCAGCATACTGCGCGT9460.10166435073986071No Hit
CAGTTATATCGTATGATGGCCTTAGAGAATATTGGGCAGAGTCCGTGAAG9370.10069714232901635No Hit
GCATAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTGTGCTGGTACCAGGCTAGGTAGGTGCTGCTAAGACTTTCA9360.10058967472781144No Hit
GCACAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGCTGCATCTACCAGGGCCACTGGCATCCCAGACAGGTT9330.10026727192419665No Hit
GTTAGGACGCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCCTCCTCCTGCTACTCTGGCTCCCAGATACCA9320.10015980432299174No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)