FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685210

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685210
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences687722
Sequences flagged as poor quality0
Sequence length18-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC25440.3699169141019191No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTAT25050.36424601801309253No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCT18320.26638670858282854No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC16640.24195823312326784No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG16060.23352459278603854No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG16010.2327975548259326No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC15660.22770828910519078No Hit
GTCCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGG15420.22421850689668207No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG15240.2216011702403006No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA14560.21171345398285935No Hit
GATCTATGCTGCCTCCTTTTTGCAAAGTGGCGTCCCATCAAGGTTCAGCGGCAGTGGCTCTGGGGCAGAATGC14450.21011397047062622No Hit
GTATGCAGACTCCGTGAGGGGCCGATTCACCATCTCCAGAGACAGTTCCCAGAACACGGTGCATCTGCAAA14420.20967774769456266No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG13960.20298899846158766No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACT13590.1976089175568035No Hit
GTCTCCATCCTTCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGTACATTAGCAA13270.19295587461212524No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGTACATTAGCAATTATTTCGCCTGGTATCAGCAAAAA13170.1915017986919133No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT12960.18844823925946821No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG12510.18190489761851447No Hit
ATAATACACAGCCGTGTCTTCACGTCTCAGGGTGTCCATTTGCAGATGCA12410.1804508216983025No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCC12320.17914215337011177No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAAC12220.1776880774498998No Hit
GTACATTAGCAATTATTTCGCCTGGTATCAGCAAAAACCGGGGAAAGCCCCTAAGCTCCTGATCTATGCTGCCTC12200.17739726226585742No Hit
GCATAGATCAGGAGCTTAGGGGCTTTCCCCGGTTTTTGCTGATACCAGGCGAAATAATTGCTAATGTACTGAC11910.1731804420972428No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA11510.167364138416395No Hit
CAATAATACACAGCCGTGTCTTCACGTCTCAGGGTGTCCATTTGCAGATGCACCGTGTTCTGGGAACTGTCTC11340.1648922093520347No Hit
CCCTAAGACTCTCCTGTGCAGCCTCTGGATTTATTTTCAGTAATTTTGCC11190.16271109547171678No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATATCCACTATGGTCC11100.16140242714352604No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT11050.16067538918342006No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAGATGTGACA11000.15994835122331408No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAGTTGAGGGAGATGGGGTAGGTGGAGT10900.15849427530310212No Hit
GTGTATTATTGTGCGAAAGAACGGCGTGGCTGGTACCACGACGCCTGGGGCCAGGGAACCCTGGTCACCGTCTC10880.15820346011905972No Hit
CTATGGTCCCAGGGCCGAAAGTGAAGGGAGGGTGAACACCAAGTTGCTGA10870.15805805252703853No Hit
GTATTATTGTGCGAAAGAACGGCGTGGCTGGTACCACGACGCCTGGGGCC10690.15544071587065703No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAGATG10690.15544071587065703No Hit
GATCAGGAGCTTAGGGGCTTTCCCCGGTTTTTGCTGATACCAGGCGAAAT10550.1534050095823603No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC10420.15151471088608479No Hit
ACTTATTACTGTCAGCAACTTGGTGTTCACCCTCCCTTCACTTTCGGCCC10150.14758870590151252No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG10060.14628003757332178No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG9940.14453514646906745No Hit
AGTCAGTACATTAGCAATTATTTCGCCTGGTATCAGCAAAAACCGGGGAAAGCCCCTAAGCTCCTGATCTA9610.139736695932368No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT9600.13959128834034681No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC9590.13944588074832565No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC9480.1378463972360925No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT9460.1375555820520501No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA9450.13741017446002893No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAGATGTGACATCCAGTTGACCCAGTCTCCATCCTTC9360.13610150613183816No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG9290.1350836529876898No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT9140.13290253910737188No Hit
GTGCAGCCACAGTTCGTTTGATATCCACTATGGTCCCAGGGCCGAAAGTGAAGGGAGGGTGAACACCAAGT9130.13275713151535068No Hit
CAGTAATAAGTTGCAGAATCTTCAGGCTGCAGATTGTTGATTGTGAGAGAGCATTCTGCCCCAGAGCCACTGCCG9100.1323209087392871No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCA8870.1289765341227996No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC8860.12883112653077844No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC8340.12126993174567631No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTG8160.11865259508929481No Hit
GTAATAAGTTGCAGAATCTTCAGGCTGCAGATTGTTGATTGTGAGAGAGCATTCTGCCCCAGAGCCACTGCCG8030.11676229639301927No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAGTGTCCTGCCGGGCTGTGCCGAGCCA7960.1157444432488709No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGG7950.11559903565684972No Hit
GCACTGAACACAGAGGGCTCACCATGGAGTTTGGGCTGAGCTGGGTTTTC7950.11559903565684972No Hit
CCCTTCACTTTCGGCCCTGGGACCATAGTGGATATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCG7940.11545362806482853No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC7840.11399955214461657No Hit
GCTTAGGGGCTTTCCCCGGTTTTTGCTGATACCAGGCGAAATAATTGCTAATGTACTGACTGGCCCGGCAAGTGA7790.11327251418451059No Hit
CCCTGAGACGTGAAGACACGGCTGTGTATTATTGTGCGAAAGAACGGCGT7750.11269088381642584No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC7700.11196384585631985No Hit
GTCTGCATACCATTCATTATTTCCATCATATGATATAACTGCCACCCATT7690.11181843826429864No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC7680.11167303067227746No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC7660.11138221548823507No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC7650.11123680789621387No Hit
CTCCTGATCTATGCTGCCTCCTTTTTGCAAAGTGGCGTCCCATCAAGGTTCAGCGGCAGTGGCTCTGGGGCAGAA7640.11109140030419269No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGG7610.1106551775281291No Hit
CTCCTGTGCAGCCTCTGGATTTATTTTCAGTAATTTTGCCATGCACTGGGTCCGCCAGGCTCCAGGCAAGGGG7600.11050976993610791No Hit
GTGTTCACCCTCCCTTCACTTTCGGCCCTGGGACCATAGTGGATATCAAACGAACTGTGGCTGCACCATCTGTCT7550.10978273197600193No Hit
GGACCATAGTGGATATCAAACGAACTGTGGCTGCACCATCTGTCTTCATC7500.10905569401589595No Hit
GTGGCGTCCCATCAAGGTTCAGCGGCAGTGGCTCTGGGGCAGAATGCTCT7430.1080378408717476No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA7330.10658376495153565No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA7310.10629294976749325No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT7300.10614754217547206No Hit
GTGAAGGGAGGGTGAACACCAAGTTGCTGACAGTAATAAGTTGCAGAATC7250.10542050421536608No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTT7220.10498428143930251No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA7140.10382102070313295No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC7140.10382102070313295No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT7130.10367561311111176No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATATCCACTA7020.10207612959887863No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCCGGTCGGTGCAGTGACAGTCGGGGG6920.10062205367866667No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA6900.10033123849462428No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA6890.10018583090260309No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGATGTA400.001605031875.9865270
GCCGACG550.00566973955.2629270
TCAACGT200.00796852850.716613
GGTAGTT807.244489E-848.02972469
TCTGGAG7300.047.18797770
TGTTAGA8200.044.47991670
CTTATAC1900.042.7118261
TTATACA1950.041.6136252
TATACAC1950.041.6136253
GTATGCC2000.041.14769442
CGTATGC1950.040.40260341
ATGCCGT1900.039.79781744
TCGTATG2000.039.35485540
GCTTGAC350.001487336438.644041
TGCTTGA2000.038.49648355
CCAACCG655.664642E-736.96830443
CGAGACA2200.036.92507623
TCCGAGC2200.036.8901713
TGTCGTA655.9048216E-736.77286538
GTATCAA9650.036.4415251
Produced by FastQC (version 0.11.5)