FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685211

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685211
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences687722
Sequences flagged as poor quality0
Sequence length18-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTA24490.3561031928599056No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT24250.35261341065139695No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG21270.30928194822908095No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC17590.25577195436528133No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTT16540.2405041572030559No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGC15260.22189198542434296No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGTTA15040.21869301839987668No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG14950.21738435007168594No Hit
GATCTATGCTGCCTCCTTTTTGCAAAGTGGCGTCCCATCAAGGTTCAGCGGCAGTGGCTCTGGGGCAGAATGCT14870.2162210893355164No Hit
GTCCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG14260.20735122622222352No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTC13880.20182573772541812No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT13220.19222883665201929No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAA13070.19004772277170134No Hit
GTCTCCATCCTTCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTT13030.1894660924036166No Hit
GTATGCAGACTCCGTGAGGGGCCGATTCACCATCTCCAGAGACAGTTCCCAGAACACGGTGCATCTGCAAATG12950.18830283166744702No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCC12700.18466764186691717No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT12300.17885133818606938No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGTACATTAGCAATTATTTCGCCTGGTATCAGCAAAAA11590.16852739915256457No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCA11510.167364138416395No Hit
GTACATTAGCAATTATTTCGCCTGGTATCAGCAAAAACCGGGGAAAGCCCCTAAGCTCCTGATCTATGCTGCCTC11390.16561924731214064No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAGATGT10840.15762182975097497No Hit
ATAATACACAGCCGTGTCTTCACGTCTCAGGGTGTCCATTTGCAGATGCACCGTGTTCTGGGAACTGTCTCTGGA10630.15456827031852988No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACT10560.1535504171743815No Hit
GCATAGATCAGGAGCTTAGGGGCTTTCCCCGGTTTTTGCTGATACCAGGCGAAATAATTGCTAATGTACTGACTG10560.1535504171743815No Hit
GTGTATTATTGTGCGAAAGAACGGCGTGGCTGGTACCACGACGCCTGGGGCCAGGGAACCCTGGTCACCGTCTC10530.15311419439831794No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT10490.15253256403023316No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG10190.1481703362695973No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGA10150.14758870590151252No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATATCCACTATGGTCCCAGGGCCGAAAGTGAAGGGAGGGT10090.14671626034938537No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT10070.14642544516534298No Hit
CTATGGTCCCAGGGCCGAAAGTGAAGGGAGGGTGAACACCAAGTTGCTGACAGTAATAAGTTGCAGAATCTTCAG9850.1432264781408767No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACA9680.14075454907651638No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT9570.13915506556428325No Hit
CCCTAAGACTCTCCTGTGCAGCCTCTGGATTTATTTTCAGTAATTTTGCCATGCACTGGGTCCGCCAGGCTCCAG9520.13842802760417727No Hit
ACTTATTACTGTCAGCAACTTGGTGTTCACCCTCCCTTCACTTTCGGCCC9520.13842802760417727No Hit
GTATTATTGTGCGAAAGAACGGCGTGGCTGGTACCACGACGCCTGGGGCC9480.1378463972360925No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG9470.1377009896440713No Hit
GATCAGGAGCTTAGGGGCTTTCCCCGGTTTTTGCTGATACCAGGCGAAATAATTGCTAATGTACTGACTGGCCCG9400.13668313649992295No Hit
CTCCTGATCTATGCTGCCTCCTTTTTGCAAAGTGGCGTCCCATCAAGGTT9330.13566528335577457No Hit
CAATAATACACAGCCGTGTCTTCACGTCTCAGGGTGTCCATTTGCAGATGCACCGTGTTCTGGGAACTGTCTCTG9210.13392039225152025No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC9140.13290253910737188No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGACTTGCCGGCTAGGCACTGTGTGGCC9080.1320300935552447No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAGATGTGACAT8980.13057601763503277No Hit
GTGCAGCCACAGTTCGTTTGATATCCACTATGGTCCCAGGGCCGAAAGTGAAGGGAGGGTGAACACCAAGTT8910.12955816449088442No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTTCCATGGCTCGGCACAGCCCGGCAG8840.12854031134673605No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC8760.12737705061056648No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA8730.1269408278345029No Hit
AGTCAGTACATTAGCAATTATTTCGCCTGGTATCAGCAAAAACCGGGGAAAGCCCCTAAGCTCCTGATCTAT8690.1263591974664181No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG8610.12519593673024854No Hit
CCCTTCACTTTCGGCCCTGGGACCATAGTGGATATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCC8530.12403267599407901No Hit
GTAATAAGTTGCAGAATCTTCAGGCTGCAGATTGTTGATTGTGAGAGAGCATTCTGCCCCAGAGCCACTGCC8460.12301482284993064No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAGATGTGACATCCAGTTGACCCAGTCTCCATCCTTC8450.12286941525790945No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAG8240.11981585582546436No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG8120.11807096472121001No Hit
CCCTGAGACGTGAAGACACGGCTGTGTATTATTGTGCGAAAGAACGGCGT7940.11545362806482853No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAA7930.11530822047280732No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT7920.11516281288078613No Hit
GTGTTCACCCTCCCTTCACTTTCGGCCCTGGGACCATAGTGGATATCAAA7920.11516281288078613No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA7780.11312710659248941No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA7750.11269088381642584No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCT7680.11167303067227746No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTA7620.1108005851201503No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAACGTGGTCATCGCCTGCCTGGTCCAGG7580.1102189547520655No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTAC7460.10847406364781118No Hit
CAGTAATAAGTTGCAGAATCTTCAGGCTGCAGATTGTTGATTGTGAGAGAGCATTCTGCCCCAGAGCCACTGCC7450.10832865605578999No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA7370.10716539531962044No Hit
CTCCTGTGCAGCCTCTGGATTTATTTTCAGTAATTTTGCCATGCACTGGGTCCGCCAGGCTCCAGGCAAGGG7360.10701998772759923No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA7340.10672917254355685No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC7240.1052750966233449No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC7240.1052750966233449No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC7100.10323939033504818No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT7100.10323939033504818No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG7040.102366944782921No Hit
GCACTGAACACAGAGGGCTCACCATGGAGTTTGGGCTGAGCTGGGTTTTC7040.102366944782921No Hit
GGACCATAGTGGATATCAAACGAACTGTGGCTGCACCATCTGTCTTCATC7040.102366944782921No Hit
GTGAAGGGAGGGTGAACACCAAGTTGCTGACAGTAATAAGTTGCAGAATCTTCAGGCTGCAGATTGTTGATTGT6930.10076746127068786No Hit
GTCTGCATACCATTCATTATTTCCATCATATGATATAACTGCCACCCATTCCAGCCCCTTGCCTGGAGCCTGGCG6930.10076746127068786No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGG6890.10018583090260309No Hit
GTGGCGTCCCATCAAGGTTCAGCGGCAGTGGCTCTGGGGCAGAATGCTCTCTCACAATCAACAATCTGCAGCCT6880.1000404233105819No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCGTA150.0027734188198.4032170
GTAGTTT600.001744039974.4012170
TTCCCGT700.003213771963.77245770
GGTTACC200.00751699451.47045543
GTCGGTT200.00801425450.6432412
GCCCCAT900.00868835549.60080370
AAACTCA1255.3091266E-447.6167770
GACAGGT858.185452E-1139.7635651
GAGCCAT7600.039.15852770
TATTGTT1951.0254489E-438.15446570
TTGGTTT1650.002080664536.0733170
ATAGCGG400.002214205735.63582263
GGTAGTT650.0024957934.7736969
GTATCAA9450.034.6931881
GAGCAAA14500.033.86537670
GACAGAC808.488496E-833.799031
GCCGTTA400.002882739833.76952715
ACAACAC400.00288788333.757253
ATACTTT400.00288788333.757256
CTCCTCA8850.033.62766370
Produced by FastQC (version 0.11.5)