FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685212

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685212
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences832565
Sequences flagged as poor quality0
Sequence length18-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTATGGTGGGTCCTTCAGTGGTTACTACTGGAGCTGGATCCGCCAGCCCCCAGGGAAGGGGCTGGAGTGGAT45010.5406184502110947No Hit
GTAATACACAGCCGTGTCCGCGGCGGTCACAGAATCCAACTTCAGGGAGAACTGGTTCTTGGACGCGTCTACTGA42570.5113114291376649No Hit
GTTCTGGGGTGTCTCCACCATGGCCTGGACCCCTCTCTGGCTCACTCTCCTCACTCTTTGCATAGGTTCTGTGGT28750.3453183835496328No Hit
GTGTATTACTGTGCGAGAGGAGGGAAGTCTTATTGTGCTGGTGGTGTTTG27380.3288632118813546No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGG27340.3283827689129377No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAG18120.2176406646928468No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG16940.20346759712454882No Hit
CAGTAATACACAGCCGTGTCCGCGGCGGTCACAGAATCCAACTTCAGGGAGAACTGGTTCTTGGACGCGTCTA15520.18641187174574958No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT15140.1818476635457892No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA15130.181727552803685No Hit
GTCATGGACCTCCTGCACAAGAACATGAAACACCTGTGGTTCTTCCTCCTCCTGGTGGCAGCTCCCAGATGGGTC14800.17776389831424574No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACTACATTTACATTATTATTACTAGTGTCC14780.17752367683003728No Hit
CTTATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGT14670.17620245866689088No Hit
CAATAAGACTTCCCTCCTCTCGCACAGTAATACACAGCCGTGTCCGCGGC14570.17500135124584867No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAGTTGAGGGAGATGGGGTAGGTGGAGT14110.16947625710905456No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG13970.16779470671959548No Hit
GTCCTGGCTTCTGCTGGTACCAGCTTGCATAATATCTTCTGAGGCTGTCTCCTTGGCATGTCATCCTGACTG13960.16767459597749124No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTT13130.15770540438284097No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT13100.15734507215652832No Hit
GTATTACTGTGCGAGAGGAGGGAAGTCTTATTGTGCTGGTGGTGTTTGCT13020.15638418621969458No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG13010.15626407547759033No Hit
CTCTTTGCATAGGTTCTGTGGTTTCTTCTGAGCTGACTCAGGACCCTGCTGTGTCTGTGGCCTTGGGACAGACAG12770.15338141766708904No Hit
GTAATAGTCAGCCTGATCTTCCGCCTGAGCCCCAGTGATGGTCAAGGAAG12750.1531411961828806No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACTACATTTACATTA12670.15218031024604683No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT12540.15061887059869197No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC12500.15013842763027513No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC12390.1488172094671287No Hit
CTCCTCACTCTTTGCATAGGTTCTGTGGTTTCTTCTGAGCTGACTCAGGACCCTGCTGTGTCTGTGGCCTTGGGA12040.144613333493481No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG12000.14413289052506412No Hit
GTACAAACACCTACTACAACCCGTCCCTCAAGAGTCGTGTCACCATGTCAGTAGACGCGTCCAAGAACCAGTTC11850.1423312293935008No Hit
AGCCAGGACAGGCCCCTGTATTTGTCATGTATGGTAAAAACAACCGGCCCTCAGGGATCCCAGACCGATTCTC11760.14125023271456283No Hit
GTATTTGTCATGTATGGTAAAAACAACCGGCCCTCAGGGATCCCAGACCG11670.14016923603562484No Hit
GTCTTATTGTGCTGGTGGTGTTTGCTTCTGGTTCGACCCCTGGGGCCAGG11660.14004912529352062No Hit
CAGTAATAGTCAGCCTGATCTTCCGCCTGAGCCCCAGTGATGGTCAAGGA11620.13956868232510375No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGG11600.1393284608408953No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGG11380.13668602451460246No Hit
GTGTTTGTACTGGTGCTTCCACTAGGATTGATTTCCCCAATCCACTCCAGCCCCTTCCCTGGGGGCTGGCGGATC11190.13440392041462226No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC11060.1328424807672674No Hit
ATTATTACTAGTGTCCCGGGAATTACAGTAATAGTCAGCCTGATCTTCCG10940.13140115186201679No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG10920.13116093037780835No Hit
CCCTATCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCAGTGGTTACTAC10770.12935926924624502No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG10630.12767771885678597No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC10600.1273173866304733No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT10530.12647661143574376No Hit
ACCATAGACAGCGCAGGTGAGGGATAGGGTCTCCGAAGGCTTCAACAGTCCTGCGCCCCACTGCTGTAGCTGCAC10440.12539561475680577No Hit
GGGTTCCCTGGCCCCAGGGGTCGAACCAGAAGCAAACACCACCAGCACAA10270.12335373214103403No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAGTGTCCTGCCGGGCTGTGCCGAGCCA10180.12227273546209605No Hit
ATACAGGGGCCTGTCCTGGCTTCTGCTGGTACCAGCTTGCATAATATCTTCTGAGGCTGTCTCCTTGGCATGTCA9850.11830908097265679No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTATGCGGCCAGCAGCTACCTGAGCCTGACGCCTGAGCAG9620.11554653390425972No Hit
ACTCAGGACCCTGCTGTGTCTGTGGCCTTGGGACAGACAGTCAGGATGACATGCCAAGGAGACAGCCTCAGAAGA9550.11470575870953018No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATGGGTGGGTTTACCCGCCAAGCGG9490.11398509425690487No Hit
CCTTGGGGCTGGTCGGGGATGCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGGGGTCGAACCAGAAGCAAA9490.11398509425690487No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC9480.11386498351480065No Hit
TGACAAATACAGGGGCCTGTCCTGGCTTCTGCTGGTACCAGCTTGCATAA9450.11350465128848798No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC9440.11338454054638378No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC9410.11302420832007111No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC9400.11290409757796689No Hit
GTCTGTGGCCTTGGGACAGACAGTCAGGATGACATGCCAAGGAGACAGCCTCAGAAGATATTATGCAAGCTGGTA9370.11254376535165422No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGC9190.11038177199377826No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA9180.11026166125167405No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT9170.11014155050956982No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT9150.1099013290253614No Hit
ATATTATGCAAGCTGGTACCAGCAGAAGCCAGGACAGGCCCCTGTATTTGTCATGTATGGTAAAAACAACCGGCC9100.10930077531484028No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAGGTCCCCGGAGGCATCCTGGCTGGG9100.10930077531484028No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA9060.1088203323464234No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCATACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA9010.1082197786359023No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC8940.10737900344117277No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC8900.10689856047275588No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC8850.10629800676223479No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGC8780.10545723156750525No Hit
GCATAATATCTTCTGAGGCTGTCTCCTTGGCATGTCATCCTGACTGTCTGTCCCAAGGCCACAGACACAGCAGGG8720.10473656711487993No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC8600.10329523820962927No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA8560.10281479524121241No Hit
GTACGGGAGCTGTGGGCTCAGAAGCAGAGTTCTGGGGTGTCTCCACCATGGCCTGGACCCCTCTCTGGCTCAC8520.10233435227279553No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG8500.10209413078858708No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCC8470.10173379856227441No Hit
GTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGG8420.10113324485175332No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCCGGTCGGTGCAGTGACAGTCGGGGG8340.10017235891491955No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG2450.0107.7902670
CTAGTAC150.002499844967.9713826
AAAACCG1105.0949893E-967.22192470
AATGGTA3050.062.3416570
GGGTCTA200.00784008350.9264835
CGCGTAT352.3792758E-548.90382839
GTGGTAT2100.048.515981
CGATTAA306.492656E-445.73497841
TCGCGTA306.588347E-445.5993938
GGCCAGG13450.045.5524170
TCTCGTA2500.045.1433938
ATCTCGT2400.044.14769737
TAAGTCA1452.6374018E-643.71081570
ACGAGAC3350.043.58673522
ATGCCGT3250.043.38553244
TGGTATC2350.043.3390852
CGTATGC3100.043.15316441
CTTATAC3700.043.139891
CTACTGA12300.043.1059869
ACATCGC405.3204298E-542.6745535
Produced by FastQC (version 0.11.5)