FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004685213

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685213
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences832565
Sequences flagged as poor quality0
Sequence length18-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACACAGCCGTGTCCGCGGCGGTCACAGAATCCAACTTCAGGGAGAACTGGTTCTTGGACGCGTCTACT45310.5442217724742212No Hit
GTCTATGGTGGGTCCTTCAGTGGTTACTACTGGAGCTGGATCCGCCAGCCCCCAGGGAAGGGGCTGGAGTGGATT44090.5295682619375064No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG39400.47323632389062714No Hit
GTTCTGGGGTGTCTCCACCATGGCCTGGACCCCTCTCTGGCTCACTCTCC28130.3378715175391711No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGT26130.31384936911832706No Hit
GTGTATTACTGTGCGAGAGGAGGGAAGTCTTATTGTGCTGGTGGTGTTTGCTTCTGGTTCGACCCCTGGGGCCAG26090.3133689261499102No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT17880.21475800688234553No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGG16770.2014257145087771No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG15400.18497054284049894No Hit
CAGTAATACACAGCCGTGTCCGCGGCGGTCACAGAATCCAACTTCAGGGAGAACTGGTTCTTGGACGCGTCTA14640.17584212644057823No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCA14630.175722015698474No Hit
GTATTACTGTGCGAGAGGAGGGAAGTCTTATTGTGCTGGTGGTGTTTGCTTCTGGTTCGACCCCTGGGGCCAGG14280.17151813972482627No Hit
GTCATGGACCTCCTGCACAAGAACATGAAACACCTGTGGTTCTTCCTCCTCCTGGTGGCAGCTCCCAGATGGGTC14160.17007681081957565No Hit
GTCCTGGCTTCTGCTGGTACCAGCTTGCATAATATCTTCTGAGGCTGTCTCCTTGGCATGTCATCCTGACTGT13990.1680349282038039No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAG13100.15734507215652832No Hit
CTCCTCACTCTTTGCATAGGTTCTGTGGTTTCTTCTGAGCTGACTCAGGACCCTGCTGTGTCTGTGGCCTTGG12970.15578363250917346No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACTACATTTACATTATTATTACTAGTGTCC12960.15566352176706924No Hit
CAATAAGACTTCCCTCCTCTCGCACAGTAATACACAGCCGTGTCCGCGGCGGTCACAGAATCCAACTTCAGGGA12750.1531411961828806No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACTACATTTACATTA12350.1483367664987118No Hit
CTCTTTGCATAGGTTCTGTGGTTTCTTCTGAGCTGACTCAGGACCCTGCT12290.1476161020460865No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA12240.1470155483355654No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT12200.1465351053671485No Hit
GTACAAACACCTACTACAACCCGTCCCTCAAGAGTCGTGTCACCATGTCAGTAGACGCGTCCAAGAACCAGTTCT12130.14569433017241898No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAG11900.1429317831040219No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGACTTGCCGGCTAGGCACTGTGTGGCC11830.14209100790929236No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCA11630.13968879306720797No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAG11620.13956868232510375No Hit
GTGTTTGTACTGGTGCTTCCACTAGGATTGATTTCCCCAATCCACTCCAGCCCCTTCCCTGGGGGCTGGCG11490.1380072426777489No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC11400.1369262459988109No Hit
GTAATAGTCAGCCTGATCTTCCGCCTGAGCCCCAGTGATGGTCAAGGAAGCTGTGTTTCCTGAACTGGAGCCAGA11330.13608547080408137No Hit
AGCCAGGACAGGCCCCTGTATTTGTCATGTATGGTAAAAACAACCGGCCCTCAGGGATCCCAGACCGATTCTCTG11300.1357251385777687No Hit
GTCTTATTGTGCTGGTGGTGTTTGCTTCTGGTTCGACCCCTGGGGCCAGG10980.13188159483043366No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTTCCATGGCTCGGCACAGCCCGGCAG10890.1308005981514957No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC10750.1291190477620366No Hit
CAGTAATAGTCAGCCTGATCTTCCGCCTGAGCCCCAGTGATGGTCAAGGAAGCTGTGTTTCCTGAACTGGAGCCA10680.12827827256730706No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG10640.12779782959889016No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG10550.1267168329199522No Hit
ATTATTACTAGTGTCCCGGGAATTACAGTAATAGTCAGCCTGATCTTCCG10500.12611627920943108No Hit
GTATTTGTCATGTATGGTAAAAACAACCGGCCCTCAGGGATCCCAGACCG10300.12371406436734671No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC10280.12347384288313824No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTA10210.1226330676884087No Hit
ACCATAGACAGCGCAGGTGAGGGATAGGGTCTCCGAAGGCTTCAACAGTCCTGCGCCCCACTGCTGTAGCTGCAC10150.1219124032357834No Hit
GGGTTCCCTGGCCCCAGGGGTCGAACCAGAAGCAAACACCACCAGCACAA10060.12083140655684542No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC10060.12083140655684542No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTG9940.11939007765159478No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC9710.11662753058319772No Hit
CCCTATCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCAGTGGTTACTACTGGAGCTGGATCCGCCAGCCCCCAG9640.11578675538846818No Hit
ATACAGGGGCCTGTCCTGGCTTCTGCTGGTACCAGCTTGCATAATATCTT9540.11458564796742597No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG9420.11314431906217531No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA9360.11242365460955No Hit
ACTCAGGACCCTGCTGTGTCTGTGGCCTTGGGACAGACAGTCAGGATGACATGCCAAGGAGACAGCCTCAGAA9270.11134265793061203No Hit
ATATTATGCAAGCTGGTACCAGCAGAAGCCAGGACAGGCCCCTGTATTTGTCATGTATGGTAAAAACAACCGGCC9260.1112225471885078No Hit
GTCTGTGGCCTTGGGACAGACAGTCAGGATGACATGCCAAGGAGACAGCCTCAGAAGATATTATGCAAGCTGGTA9120.10954099679904872No Hit
TGACAAATACAGGGGCCTGTCCTGGCTTCTGCTGGTACCAGCTTGCATAA8940.10737900344117277No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTATGCGGCCAGCAGCTAC8930.10725889269906853No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC8800.10569745305171367No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAACGTGGTCATCGCCTGCCTGGTCCAGG8800.10569745305171367No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGC8780.10545723156750525No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGCAGAG8750.10509689934119258No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC8630.10365557043594192No Hit
CCTTGGGGCTGGTCGGGGATGCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGGGGTCGAACCAGAAGCAAA8590.10317512746752507No Hit
GGCCTGGACCCCTCTCTGGCTCACTCTCCTCACTCTTTGCATAGGTTCTGTGGTTTCTTCTGAGCTGACTCAG8590.10317512746752507No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCT8400.10089302336754487No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)