FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685214

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685214
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1113429
Sequences flagged as poor quality0
Sequence length18-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTAT49620.4456503288489881No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC46420.41691028345767894No Hit
CTATTAAGCTCTTGACAGAAATAAGTTGCAAAATCCTCAGGCTGCAGGCTGCTGATTGTGAGAGTGAATTCTGTC40310.3620347592886479No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC35130.3155118108114662No Hit
GTCCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGG32570.29251977449841887No Hit
GCTTAATAGTTACCCTCGGACTTTCGGCCCTGGGACCAAAGTGGATATCAAACGAACTGTGGCTGCACCA31660.2843468240902653No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCT31410.2821015080440693No Hit
GATCTATGCTGCATCCACTTTGCAAGGTGGGGTCCCATCAAGGTTCCGCGGCAGTGGATCTGGGACAGAATTCA30230.271503616306024No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG29370.2637797291071097No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG28530.256235467191891No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC28490.25587621662449966No Hit
GTCTCCATCCTTCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGGACATTAACAG25980.23333324352069149No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG25600.2299203631304735No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG25340.22758523444242965No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGGACATTAACAGTTATTTAGGCTGGTACCAGCAGAAA25290.22713617123319044No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG25250.22677692066579908No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCC24820.22291497706634192No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA24680.22165760008047214No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT24480.2198613472435153No Hit
GCATAGATCAGGAGCTTAGGGGCTTCTCCTGGTTTCTGCTGGTACCAGCCTAAATAACTGTTAATGTCCTGAC23510.2111495209842747No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC23410.21025139456579628No Hit
GTAATAAATGGCCGTGTCCGCGGCGGTCACAGAGCTCAGGTTCAGGGAGAAGTGGTTCTTGGACTTGTCTACTC23320.2094430807891657No Hit
GTCTCTGGTGGCTCCATCAGCAGTAGTTACTGGTGGACTTGGGTCCGCCAGCCCCCAGGAAAGGGACTGGAG23280.20908383022177437No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACT22970.2062996383244913No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT22420.20135994302286No Hit
GTGCAGCCACAGTTCGTTTGATATCCACTTTGGTCCCAGGGCCGAAAGTC22110.19857575112557693No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAAC21080.18932504901524927No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAGATGTGACAT20880.18752879617829246No Hit
GTCCTGGACCTCCTGCACAAGAACATGAAACACCTGTGGTTCTTCCTCCTCCTGGTGGCAACTCCCAGATGGGTC20500.18411591578807449No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAGATG19890.1786373446353562No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT19450.17468558839405118No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG19070.1712727080038332No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC18910.16983570573426773No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA18330.16462657250709295No Hit
CCATAGGAGTAGACAAGTCCAAGAACCACTTCTCCCTGAACCTGAGCTCT18010.16175256796796203No Hit
GAATTCACTCTCACAATCAGCAGCCTGCAGCCTGAGGATTTTGCAACTTATTTCTGTCAAGAGCTTAATAGTTAC17820.16004612777285307No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT17230.1547471819038304No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG17110.15366943020165633No Hit
GATCAGGAGCTTAGGGGCTTCTCCTGGTTTCTGCTGGTACCAGCCTAAAT16670.14971767396035132No Hit
ATTTATTACTGTGCGAGGCTTAATTTTGGAGTTGCTATGGGTATTACTACTGTTTACTCGTACTACATGGACG16550.14863992225817724No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC16320.14657423149567686No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAGATGTGACATTCAGTTGACCCAGTCTCCATCCTTC16220.14567610507719844No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT16170.14522704186795926No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAGTTGAGGGAGATGGGGTAGGTGGAGT15910.14289191317991537No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA15700.14100584770111071No Hit
CTCCCAGATGGGTCCTGTCTCAGGTGCAGCTGCAGGAGTCGGGCCCAGGCCTGGTGGAGCCTTCGGGGACCTTG15670.1407364097755672No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC15570.13983828335708878No Hit
ACCTTGATGGGACCCCACCTTGCAAAGTGGATGCAGCATAGATCAGGAGCTTAGGGGCTTCTCCTGGTTTC15500.13920959486415388No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC15330.13768277995274059No Hit
GTAGTAATACCCATAGCAACTCCAAAATTAAGCCTCGCACAGTAATAAATGGCCGTGTCCGCGGCGGTCACAGAG15300.13741334202719707No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATATCCACTTTGGTCC15060.13525783862284887No Hit
CATCAAGGTTCCGCGGCAGTGGATCTGGGACAGAATTCACTCTCACAATCAGCAGCCTGCAGCCTGAGGATTT14560.13076720653045681No Hit
CTCCTGATCTATGCTGCATCCACTTTGCAAGGTGGGGTCCCATCAAGGTTCCGCGGCAGTGGATCTGGGACAGAA14420.12950982954458704No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCA14300.12843207784241295No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA14060.12627657443806475No Hit
GGACATTAACAGTTATTTAGGCTGGTACCAGCAGAAACCAGGAGAAGCCCCTAAGCTCCTGATCTATGCTGCATC14020.1259173238706734No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC13930.12510901009404282No Hit
CAGTTGACCCAGTCTCCATCCTTCCTGTCTGCATCTGTAGGAGACAGAGT13790.12385163310817304No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGG13660.12268406876415111No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC13620.12232481819675974No Hit
ATGCTGCATCCACTTTGCAAGGTGGGGTCCCATCAAGGTTCCGCGGCAGTGGATCTGGGACAGAATTCACTCTCA13590.12205538027121622No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC13420.12052856535980291No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTG13340.11981006422502019No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG13290.11936100101578098No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC13220.11873231252284609No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC12990.11666662176034574No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA12960.11639718383480223No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTT12920.11603793326741085No Hit
GTGGGGTCCCATCAAGGTTCCGCGGCAGTGGATCTGGGACAGAATTCACT12860.11549905741632381No Hit
GGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAGATGTGACATTCAGT12850.11540924477447596No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA12790.11487036892338892No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC12480.11208617702610583No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC12390.11127786324947528No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA12270.11020011154730118No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC12190.10948161041251844No Hit
GTCCGAGGGTAACTATTAAGCTCTTGACAGAAATAAGTTGCAAAATCCTC12130.10894273456143139No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAGTGTCCTGCCGGGCTGTGCCGAGCCA12020.10795479550110515No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC11960.10741591965001811No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTT11950.10732610700817026No Hit
AGTCAGGACATTAACAGTTATTTAGGCTGGTACCAGCAGAAACCAGGAGAAGCCCCTAAGCTCCTGATCTA11910.10696685644077888No Hit
CCCCTAAGCTCCTGATCTATGCTGCATCCACTTTGCAAGGTGGGGTCCCATCAAGGTTCCGCGGCAGTGGATC11850.10642798058969184No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA11850.10642798058969184No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT11660.10472154039458285No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAGGTCCCCGGAGGCATCCTGGCTGGG11620.10436228982719149No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGG11610.10427247718534365No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC11540.10364378869240877No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT11500.1032845381250174No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA11490.10319472548316955No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT11290.10139847264621274No Hit
CTCCTATGGTGACTCGACTCTTGAGGGACGGGTTGTAGTTGGAGGTCCCCGTATGATAGACTTCCCCAATCCAC11280.10130866000436489No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC11260.10112903472066923No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC11240.10094940943697352No Hit
GTTAATGTCCTGACTGGCCCGGCAAGTGATGGTGACTCTGTCTCCTACAGATGCAGACAGGAAGGATGGAGAC11200.10059015886958217No Hit
CCATCCTTCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGGACATTAACAGTTAT11180.10041053358588647No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAATAC353.6074016E-758.3523833
TATAGGG702.1083576E-457.4341470
CCGATGA550.005854589454.82349870
TGTTAGA10900.047.94960470
GGTATCA7300.044.7654841
GTGGTAT1650.043.3241731
GCGTAGA28650.042.44914270
TCGTATG1950.040.4554840
CGTATGC1950.038.72458641
TGGTATC1950.038.402852
GTATCAA18650.037.9646531
CCATTTA10000.037.18860670
GAGTTTA2750.035.897171
TATTCTA1401.4723034E-435.8963470
ACGAGAC2200.035.60210422
ATCTCGT1650.035.25880437
CCGTCTT2250.035.24323347
GTATGCC2450.035.04042442
TCTCGCG8950.034.8134370
TATACAC2250.034.795313
Produced by FastQC (version 0.11.5)