FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685215

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685215
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1113429
Sequences flagged as poor quality0
Sequence length18-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT49240.4422374484587701No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTA46100.414036278918548No Hit
CTATTAAGCTCTTGACAGAAATAAGTTGCAAAATCCTCAGGCTGCAGGCTGCTGATTGTGAGAGTGAATTCTGTC36650.3291633323723381No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTT32830.29485490318646274No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC32650.2932382756332016No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGC32050.2878495171223311No Hit
GTCCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG30100.27033605196200206No Hit
GATCTATGCTGCATCCACTTTGCAAGGTGGGGTCCCATCAAGGTTCCGCGGCAGTGGATCTGGGACAGAATTCA28470.2556965913408039No Hit
GCTTAATAGTTACCCTCGGACTTTCGGCCCTGGGACCAAAGTGGATATCAAACGAACTGTGGCTGCACCAT26590.23881181467340978No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTC26590.23881181467340978No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT26320.2363868733435181No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG25820.23189624125112604No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGTTA24180.21716696798808008No Hit
GTCTCCATCCTTCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTT23840.21411333816525346No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG23570.21168839683536173No Hit
GTAATAAATGGCCGTGTCCGCGGCGGTCACAGAGCTCAGGTTCAGGGAGAAGTGGTTCTTGGACTTGTCTACTC23460.2107004577750355No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCA23420.21034120720764415No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGGACATTAACAGTTATTTAGGCTGGTACCAGCAGAAA23230.20863476701253517No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG22930.20594038775709989No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAA22620.2031561958598168No Hit
GTCTCTGGTGGCTCCATCAGCAGTAGTTACTGGTGGACTTGGGTCCGCCAGCCCCCAGGAAAGGGACTGGAGTG22380.20100069245546867No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCC22160.19902481433481614No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT21850.19624062243753307No Hit
GTGCAGCCACAGTTCGTTTGATATCCACTTTGGTCCCAGGGCCGAAAGTC21680.19471380752611978No Hit
GCATAGATCAGGAGCTTAGGGGCTTCTCCTGGTTTCTGCTGGTACCAGCCTAAATAACTGTTAATGTCCTGACTG21320.19148055241959747No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCT21240.19076205128481474No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT21090.18941486165709714No Hit
GTCCTGGACCTCCTGCACAAGAACATGAAACACCTGTGGTTCTTCCTCCTCCTGGTGGCAACTCCCAGATGGGTC20780.18663066975981407No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACT20650.1854631054157921No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGA20530.18438535371361803No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT20430.1834872272951396No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC19880.17854753199350834No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAGATGT19780.17764940557502992No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACA18790.16875795403209365No Hit
GAATTCACTCTCACAATCAGCAGCCTGCAGCCTGAGGATTTTGCAACTTATTTCTGTCAAGAGCTTAATAGTTAC17260.15501661982937395No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA16630.14935842339295996No Hit
GACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAGATGTGACATTCAGTT15800.14190397411958913No Hit
ATTTATTACTGTGCGAGGCTTAATTTTGGAGTTGCTATGGGTATTACTACTGTTTACTCGTACTACATGGACGT15670.1407364097755672No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAGATGTGACATTCAGTTGACCCAGTCTCCATCCTTC15530.1394790327896974No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAG15470.13894015693861037No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT15380.1381318431619798No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC15260.13705409145980568No Hit
CTCCTGATCTATGCTGCATCCACTTTGCAAGGTGGGGTCCCATCAAGGTT14990.134629150129914No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGACTTGCCGGCTAGGCACTGTGTGGCC14930.13409027427882694No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG14740.13238383408371796No Hit
GATCAGGAGCTTAGGGGCTTCTCCTGGTTTCTGCTGGTACCAGCCTAAATAACTGTTAATGTCCTGACTGGCCCG14690.13193477087447875No Hit
GTAGTAATACCCATAGCAACTCCAAAATTAAGCCTCGCACAGTAATAAATGGCCGTGTCCGCGGCGGTCACAGA14630.1313958950233917No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCT14630.1313958950233917No Hit
GTGGGGTCCCATCAAGGTTCCGCGGCAGTGGATCTGGGACAGAATTCACTCTCACAATCAGCAGCCTGCAGCCT14560.13076720653045681No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT14510.1303181433212176No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG14440.12968945482828273No Hit
CTCCCAGATGGGTCCTGTCTCAGGTGCAGCTGCAGGAGTCGGGCCCAGGCCTGGTGGAGCCTTCGGGGACCTTGT14170.12726451349839102No Hit
ACCTTGATGGGACCCCACCTTGCAAAGTGGATGCAGCATAGATCAGGAGCTTAGGGGCTTCTCCTGGTTTCT14170.12726451349839102No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13960.12537844801958636No Hit
CATCAAGGTTCCGCGGCAGTGGATCTGGGACAGAATTCACTCTCACAATCAGCAGCCTGCAGCCTGAGGATTTT13910.12492938481034714No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA13910.12492938481034714No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCA13780.12376182046632521No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTAC13320.1196304389413245No Hit
GGACATTAACAGTTATTTAGGCTGGTACCAGCAGAAACCAGGAGAAGCCCCTAAGCTCCTGATCTATGCTGCATC13270.1191813757320853No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG13270.1191813757320853No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA13130.11792399874621551No Hit
CCATAGGAGTAGACAAGTCCAAGAACCACTTCTCCCTGAACCTGAGCTCTGTGACCGCCGCGGACACGGCCATTT12930.11612774590925869No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA12880.11567868270001948No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGG12720.11424168043045403No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG12690.1139722425049105No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTA12660.11370280457936699No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC12630.11343336665382345No Hit
ATGCTGCATCCACTTTGCAAGGTGGGGTCCCATCAAGGTTCCGCGGCAGTGGATCTGGGACAGAATTCACTCTCA12600.11316392872827993No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA12570.1128944908027364No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAA12560.11280467816088856No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT12480.11208617702610583No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT12480.11208617702610583No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA12420.11154730117501879No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA12230.1098408609799098No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC12020.10795479550110515No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTTCCATGGCTCGGCACAGCCCGGCAG12000.10777517021740946No Hit
GTCCGAGGGTAACTATTAAGCTCTTGACAGAAATAAGTTGCAAAATCCTCAGGCTGCAGGCTGCTGATTGTGAGA11700.10508079096197423No Hit
GGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAGATGTGACATTCAGTT11610.10427247718534365No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTT11400.10238641170653899No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAACGTGGTCATCGCCTGCCTGGTCCAGG11280.10130866000436489No Hit
GTGGATGCAGCATAGATCAGGAGCTTAGGGGCTTCTCCTGGTTTCTGCTG11230.10085959679512568No Hit
AGTCAGGACATTAACAGTTATTTAGGCTGGTACCAGCAGAAACCAGGAGAAGCCCCTAAGCTCCTGATCTAT11160.1002309083021908No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACTTA550.001135105882.9172770
ATGGTAA800.00501469757.00562370
GTTTGCG200.007105499552.2107157
TGGTATC1850.051.330222
GTGGTAT2000.050.9084931
AACCTTT1501.9246396E-550.67166570
GACCCAT3950.050.0302570
GAGCAAA23650.048.85048770
GTAGTTT950.00989373248.00473470
GGTATCA7600.046.8894041
GAGCCAT11050.045.39814470
AATGGTA2451.5945079E-743.43285870
ACCAGGT2355.9679187E-638.81233670
GTAGATA1600.001612596338.0037570
AAGCGGT7700.037.51019370
GTATCAA20200.036.963261
TCTTGTT3354.508547E-836.30208670
CTTGACA22400.035.96783470
CGTCACA11500.034.36860770
AAGGACA25200.033.17787670
Produced by FastQC (version 0.11.5)