FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004685218

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685218
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1124989
Sequences flagged as poor quality0
Sequence length18-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTAT41740.3710258500305336No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC34310.3049807598118737No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG29150.25911364466674786No Hit
GTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA27460.2440912755591388No Hit
GTCTCCAGCCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCAG27170.2415134725761763No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCT26700.23733565394861636No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG24850.2208910487124763No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGCCTAGAGCCTGAAGATTTTGCAGTTTATTACTGTCAGCAGCG24720.21973548185804487No Hit
GTCTCTGGTGGCTCCATCAGTAATTACTACTGGACCTGGATCCGACAGCCCCCAGGGAAGGGACTGGAATGGAT24230.21537988371441852No Hit
GTCCCAGACCCACTGCCAGTGAACCTGGCTGGGATGCCAGTGGCCCTGTT22760.20231308928353964No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAGCAGCTACTTAGCCTGGTACCAACAGAAACCTGGCCAGCCTCCTAGGC21960.1952019086408845No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC21950.19511301888285132No Hit
CTCCTAGGCTCCTCATCTATGATGCATCCAACAGGGCCACTGGCATCCCAGCCAGGTTCACTGGCAGTGGGTC21940.19502412912481812No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC21370.1899574129169263No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA21340.18969074364282673No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG21170.1881796177562625No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCC20800.1848906967090345No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCAGCTACTTAGCCTGGTA20400.1813351063877069No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT19760.17564616187358278No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT19650.1746683745352177No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG19220.17084611493979052No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGAC18470.16417938308730132No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAAC18420.16373493429713534No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT18380.1633793752650026No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG17970.1597348951856418No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT17870.15884599760530993No Hit
GCCTAGGAGGCTGGCCAGGTTTCTGTTGGTACCAGGCTAAGTAGCTGCTA17280.15360150188135174No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG17250.15333483260725214No Hit
GACTTCACTCTCACCATCAGCAGCCTAGAGCCTGAAGATTTTGCAGTTTATTACTGTCAGCAGCGTAGCAAC17030.15137925793052198No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA16680.14826811639936036No Hit
GCCTAGAGCCTGAAGATTTTGCAGTTTATTACTGTCAGCAGCGTAGCAACTGGCCTCCGTCTTTCGGCGGAGGGA16620.1477347778511612No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGACGGAGGCCAGT16570.14729032906099526No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCAGTGAACCTGGCTGGGATGCCAGTGGCCCTGTTGGATGCATCATAGA16430.14604587244853062No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC16400.14577920317443105No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGA16290.14480141583606596No Hit
GTTTATTACTGTCAGCAGCGTAGCAACTGGCCTCCGTCTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAA16180.14382362849770086No Hit
ATAATAGACGGCCGTGTCCGCAGCGGTCACAGAACTCAGCTTCAGGGAAAACTGCTCCTTGGACGTGTCCACTGA16010.14231250261113665No Hit
GTCAGAGTGTTAGCAGCTACTTAGCCTGGTACCAACAGAAACCTGGCCAGCCTCCTAGGCTCCTCATCTATGA15880.14115693575670518No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACATCTGTGGTTCTTCCTTCTCCTGGTGGCAGCTCCCAGATGGGTC15790.14035692793440646No Hit
GTAATAAACTGCAAAATCTTCAGGCTCTAGGCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCAG15110.13431242438814958No Hit
TCTATGATGCATCCAACAGGGCCACTGGCATCCCAGCCAGGTTCACTGGCAGTGGGTCTGGGACAGACTTCAC14990.1332457472917513No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACT14450.12844570035795905No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC14300.1271123539874612No Hit
GTCCTGAGAGGGGGCAAGTACGCAGCCACCTCACAGGTGCTGCTGCCTTC14200.12622345640712931No Hit
GTGTTAGCAGCTACTTAGCCTGGTACCAACAGAAACCTGGCCAGCCTCCT13630.12115674019923751No Hit
GTGTTGACACAGTCTCCAGCCACCCTGTCTTTGTCTCCAGGGGAAAGAGC13490.11991228358677285No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT13470.11973450407070647No Hit
CTGCTACTCTGGCTCCCAGATACCACCGGAGAAATTGTGTTGACACAGTC13020.11573446495921293No Hit
GCGTAGCAACTGGCCTCCGTCTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCA12880.11449000834674829No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA12700.11288999270215086No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACTTCGCCCACAGCATCCTGACCGTGTC12690.11280110294411766No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC12540.11146775657361982No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT12480.11093441802542069No Hit
CTCCTGGACCCGCCAGAATGGCGAAGCTGTGAAAACCCACACCAACATCTCCGAGAGCCACCCCAATGCCACTT12320.10951218189688965No Hit
TCTCAGGACTGATGGGAAGCCCCGGGTGCTGCTGATGTCAGAGTTGTTCT12310.10942329213885647No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG12250.10888995359065734No Hit
CCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGTG12120.10773438673622587No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA12000.1066677096398276No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG11920.10595659157556209No Hit
CAATAATAGACGGCCGTGTCCGCAGCGGTCACAGAACTCAGCTTCAGGGAAAACTGCTCCTTGGACGTGTCCA11860.10542325302736293No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA11810.104978804237197No Hit
ACAGAAACCTGGCCAGCCTCCTAGGCTCCTCATCTATGATGCATCCAACAGGGCCACTGGCATCCCAGCCAGGT11800.10488991447916379No Hit
GGGTATATCTATTACAGTGGGAGCACCAGCTACAACCCCTCCCTCAAGAGTCGAGTCACCATATCAGTGGACACG11770.10462324520506422No Hit
CACCTGCACTGTCTCTGGTGGCTCCATCAGTAATTACTACTGGACCTGGATCCGACAGCCCCCAGGGAAGGGA11750.10444546568899785No Hit
CATCTATGATGCATCCAACAGGGCCACTGGCATCCCAGCCAGGTTCACTGGCAGTGGGTCTGGGACAGACTTCA11700.1040010168988319No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)