FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685223

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685223
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1385941
Sequences flagged as poor quality0
Sequence length18-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGT56880.4104070808209007No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG45280.32670943423998566No Hit
GTCCTGGGCCCAGTCTGTGTTGACGCAGCCGCCCTCAGTGTCTGCGGCCCCAGGACAGAAGGTCACCATCTCCT42940.3098255986365942No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCACACCACCCAGGCTGTTATCCCATGTT42760.30852684205171793No Hit
GTGTTGACGCAGCCGCCCTCAGTGTCTGCGGCCCCAGGACAGAAGGTCAC37230.2686261536385748No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAG35590.2567930380874799No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGG35190.2539069123433104No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG34740.2506600208811197No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT33970.2451042288235935No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCACACCACCCAGG31580.2278596275021808No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC30140.2174695748231707No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT29150.2103264136063512No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAG29120.2101099541755385No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCA28990.2091719633086834No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTA28140.20303894610232326No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA26860.1938033437209809No Hit
GGACAGAAGGTCACCATCTCCTGCTCTGGAAGCAGCTCCAACATTGGAGCTAATTATGTTTCCTGGTACCAGCA26630.19214382141808345No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC26540.19149444312564531No Hit
CCCAGGAACAGCCCCCAAGCTCCTCATTTATGACAATGATAAGCGACCCTCAGGGATTCCTGACCGATTCTCTG25440.18355759732917923No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTG25080.1809600841594267No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC24720.17836257098967417No Hit
AGGCAGGACTCAGGACAATCTTCATCATGACCTGCTCCCCTCTCCTCCTCACCCTTCTCATTCACTGCACAGGGT23740.17129156291645894No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCA23350.16847759031589368No Hit
GTGTGGTATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCC23300.16811682459787247No Hit
GTATCAACGCAGAGTACGGGAAGCTCTGCTTCAGCTGTGAGCGCAGAAGGCAGGACTCAGGACAATCTTCATCAT22930.16544715828451573No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA22610.16313825768918014No Hit
GTTCCTGGGAGCTGCTGGTACCAGGAAACATAATTAGCTCCAATGTTGGAGCTGCTTCCAGAGCAGGAGATGGT22240.16046859137582337No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCT22150.15981921308338523No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGG21340.153974808451442No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATAC21280.1535418895898166No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGG20540.14820255696310303No Hit
GTAATAATCGGCCTCGTCCCCAGTCTGGAGTCCGGTGATGCCCAGGGTGGCTGACGTGCCAGACTTGGAGCCAGA20500.1479139443886861No Hit
GATTATTACTGCGGAACATGGGATAACAGCCTGGGTGGTGTGGTATTCGGCGGAGGGACCAAGCTGACCGTCCTA20040.1445948997828912No Hit
CCCTCAGTGTCTGCGGCCCCAGGACAGAAGGTCACCATCTCCTGCTCTGG19820.14300753062359797No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC19790.14279107119278528No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTG19670.14192523346953442No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGAT19610.141492314607909No Hit
GGATAACAGCCTGGGTGGTGTGGTATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCC19350.13961633287419883No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTT19310.13932772029978188No Hit
ATACCACACCACCCAGGCTGTTATCCCATGTTCCGCAGTAATAATCGGCCTCGTCCCCAGTCTGGAGTCCGGT19240.1388226482945522No Hit
GGACAATCTTCATCATGACCTGCTCCCCTCTCCTCCTCACCCTTCTCATTCACTGCACAGGGTCCTGGGCCCAGT19130.1380289637149056No Hit
GCACAGGGTCCTGGGCCCAGTCTGTGTTGACGCAGCCGCCCTCAGTGTCT18580.13406054081667257No Hit
GGGCATCACCGGACTCCAGACTGGGGACGAGGCCGATTATTACTGCGGAACATGGGATAACAGCCTGGGTGGTGT18360.13247317165737935No Hit
GTATTACCACTACCACTAATGTATGAAATCGACTCCAGCCCCTTCCCTGG18190.1312465682161073No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC17900.12915412705158447No Hit
ACTCAGGACAATCTTCATCATGACCTGCTCCCCTCTCCTCCTCACCCTTCTCATTCACTGCACAGGGTCCTGGGC17690.12763891103589545No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC17580.12684522645624888No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACG17500.12626800130741495No Hit
GTCTGGAGTCCGGTGATGCCCAGGGTGGCTGACGTGCCAGACTTGGAGCCAGAGAATCGGTCAGGAATCCCTGAG17410.1256186230149768No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCACACCACCCAGGC17280.12468063214812175No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTT17060.12309326298882853No Hit
CTCCTGCTCTGGAAGCAGCTCCAACATTGGAGCTAATTATGTTTCCTGGTACCAGCAGCTCCCAGGAACAGCCCC16990.12258819098359887No Hit
GGGTGGCTGACGTGCCAGACTTGGAGCCAGAGAATCGGTCAGGAATCCCT16530.11926914637780398No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGC16140.11645517377723873No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTG15560.11227029144819296No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAG15240.10996139085285737No Hit
GAGTACGGGAAGCTCTGCTTCAGCTGTGAGCGCAGAAGGCAGGACTCAGGACAATCTTCATCATGACCTGCTCCC15070.10873478741158535No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGC15020.10837402169356415No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG14990.10815756226275144No Hit
ACTCCAGACTGGGGACGAGGCCGATTATTACTGCGGAACATGGGATAACAGCCTGGGTGGTGTGGTATTCGGCG14980.10808540911914721No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAA14700.10606512109822856No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC14620.10548789594939467No Hit
GGGTCCTGGGCCCAGTCTGTGTTGACGCAGCCGCCCTCAGTGTCTGCGGCCCCAGGACAGAAGGTCACCATCTC14580.10519928337497772No Hit
GCTTCAGCTGTGAGCGCAGAAGGCAGGACTCAGGACAATCTTCATCATGA14560.10505497708776924No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTAT14260.10289038277964213No Hit
GTGTGACCTGGAGCGAAAGCGGACAGAACGTGACCGCCAGAAACTTCCCACCTAGCCAGGATGCCTCCGGGGAC13920.10043717589709807No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATATA150.0029357867194.6983270
ATGTAGT6450.0110.93275570
TACGTAT652.2957205E-589.8607670
AATGGTA3700.078.9317570
GTCTTAT700.0034663162.581670
TATACTA750.004555471758.40949270
TAACGGG200.005700438355.20205766
CGCAGTT800.00588113854.75890470
GTTTGGT2802.5283953E-1046.936270
CATGTAG6300.042.8090369
AGTAATA28050.041.12611470
TGACAGT30600.040.56214570
GGTAGAA32950.040.3282570
TGGTATC4850.040.235842
GGTATCA13200.039.543641
CAGCCAT12250.039.33770
CATAAAT17150.039.16671870
ACATCCA3751.03682396E-1038.93966370
GGTACGT656.2300132E-638.58773868
GTATCAA33150.037.8917051
Produced by FastQC (version 0.11.5)