FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685224

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685224
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences825550
Sequences flagged as poor quality0
Sequence length18-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAGACGGCCGTGTCGTCAGATGTTAGTCTGCTCAGCTCCATGTAGGCTGTGCTGATGGACGTGTCCCTGG27310.3308097631881776No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC22540.27303010114469145No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTAT22120.2679425837320574No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC16570.20071467506510812No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCT15480.1875113560656532No Hit
GTTTTGGCCAGGGGACCAAGTTGGAGATCAAACGAACTGTGGCTGCACCA14890.1803646054145721No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGT14520.17588274483677546No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC14330.17358124886439344No Hit
GTCTATTACTGTGCGAGTCCCTCAGGGTCCTTTGGTTCAGGGACTTATCA14290.17309672339652354No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA13530.16389073950699534No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG13440.16280055720428807No Hit
CCCTTGGACAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAGAGCCTCGTA13200.15989340439706862No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA13080.1584398279934589No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG12780.15480588698443462No Hit
GTTTATTACTGCATGCAAGCTACACAATTTCCGTACAGTTTTGGCCAGGGGACCAAGTTGGAGATCAAACGAA12500.1514142087093453No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTAGGTGGGAAGTTTCTGGCGGT12460.15092968324147538No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG12340.14947610683786566No Hit
GTAATAAACTCCGACATCTTCAGCTTCCACCCTGCTGATTTTCAGTGTGAAATCTGTCCCTGCCCCACTGCCA12150.1471746108654836No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAGTGTCCTGCCTGGCTGTGCCCAGCCA12130.14693234813154865No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATGGGTGGGTTTACCCGCCAAGCGG12110.14669008539761372No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG12080.14632669129671128No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT12020.14559990309490642No Hit
GAATTAGGACTCCTCAGGTCACCTTCTCACAATGAGGCTCCTTGCTCAGCTTCTGGGGCTGCTAATGCTCTGGG11940.14463085215916663No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCC11730.14208709345284962No Hit
GCCTACATGGAGCTGAGCAGACTAACATCTGACGACACGGCCGTCTATTACTGTGCGAGTCCCTCAGGGTCCTT11710.14184483071891466No Hit
GTGCAGCGACAGTCGGGGGTGGCAGCATGGGGGAGGTGGGGGAACTGGGC11460.13881654654472775No Hit
GTCTGGGGCTGAGGTGAAGAAGCCTGGGGCCTCAGTGAAGGTCTCCTGTAAGGCTTCTGGATACACCTTCACCGA11170.13530373690267095No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG10670.12924716855429713No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT10590.12827811761855734No Hit
GTCCCAGACAGATTCAGTGGCAGTGGGGCAGGGACAGATTTCACACTGAAAATCAGCAGGGTGGAAGCTGAAGA10230.12391738840772817No Hit
GGCCAAAACTGTACGGAAATTGTGTAGCTTGCATGCAGTAATAAACTCCGACATCTTCAGCTTCCACCCTGCTGA10200.12355399430682575No Hit
GTGTGACCTGGAGCGAAAGCGGACAGAACGTGACCGCCAGAAACTTCCCACCTAGCCAGGATGCCTCCGGGGAC10160.12306946883895585No Hit
CCCTAAAAGTGGTGACACAGACTATACACAGAAATTTCAGGGCAGGGTCACCATGACCAGGGACACGTCCATCAG10160.12306946883895585No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT10040.12161589243534614No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT9920.12016231603173641No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACT9880.11967779056386652No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCATGCGACGACCACGTTCCCATCTTGGGGGG9720.1177396886923869No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG9440.11434801041729757No Hit
GTCTGGGACCCCAGAGAACCGGTTAGAAGTCTTATAAATTAGGAGTCTTGGAGGCTGGCCTGGCCTCAGCTGA9320.11289443401368784No Hit
GGTCTAGTCAGAGCCTCGTACACGCTGATGGAAACACCTACTTGAGTTGGCATCAGCTGAGGCCAGGCCAGCCTC9260.11216764581188299No Hit
CCCCTGGACAAGGGCTTGAGTGGATGGGACGGATCCACCCTAAAAGTGGT8990.10889709890376112No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAAC8860.10732239113318393No Hit
GTATCAACGCAGAGTACGGGAATTAGGACTCCTCAGGTCACCTTCTCACA8860.10732239113318393No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCTGGTGCCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG8850.10720125976621646No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCCGGTCGGTGCAGCGACAGTCGGGGG8820.10683786566531403No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT8810.10671673429834656No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC8770.10623220883047665No Hit
CAGTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCC8600.10417297559202955No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAACTTGGTCCCCTGGCCAAAACTG8510.10308279328932228No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCGGA450.002057327771.37984570
TGCACGA150.002395511668.7059341
CGTATGC3100.045.4345741
TCGTATG3150.044.57979240
ATGCCGT3200.043.21370744
CTAGGCA3150.042.5262728
ACTAGGC3100.042.10112827
GTATGCC3400.041.50856842
GAGACTA3300.041.4793424
GACTAGG3250.041.1551526
AGACTAG3250.041.12016325
ATCTCGT3150.040.9887237
GGTATCA8100.040.750481
CACGAGA3200.040.6445621
TAGGCAT3250.040.21669829
CCCACGA3250.040.00713719
CTTGAAA3700.039.68213757
TTATACA3550.039.4269332
ATCTCCG3400.039.20842710
AAAGGGG3350.038.4687368
Produced by FastQC (version 0.11.5)