FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685225

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685225
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences825550
Sequences flagged as poor quality0
Sequence length18-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG29140.35297680334322573No Hit
GTAATAGACGGCCGTGTCGTCAGATGTTAGTCTGCTCAGCTCCATGTAGGCTGTGCTGATGGACGTGTCCCTGGT28570.3460723154260796No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTA21480.26019017624613894No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT20330.24626006904487915No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC17130.2074980316152868No Hit
GTCTATTACTGTGCGAGTCCCTCAGGGTCCTTTGGTTCAGGGACTTATCA14680.1778208467082551No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGC14000.16958391375446671No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTT13950.16897825691962934No Hit
GTTTTGGCCAGGGGACCAAGTTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCT13840.1676458118829871No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGTTA13090.15856095936042638No Hit
GTCTGGGGCTGAGGTGAAGAAGCCTGGGGCCTCAGTGAAGGTCTCCTGTAAGGCTTCTGGATACACCTTCACC12980.15722851432378415No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG12430.15056628914057293No Hit
GTAATAAACTCCGACATCTTCAGCTTCCACCCTGCTGATTTTCAGTGTGAAATCTGTCCCTGCCCCACTGCCA12230.14814366180122343No Hit
CCCTTGGACAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAGAGCCTCGTACACGCTGATGGAAACACCTACTTGA11980.14511537762703652No Hit
GTTTATTACTGCATGCAAGCTACACAATTTCCGTACAGTTTTGGCCAGGGGACCAAGTTGGAGATCAAACGAA11970.14499424626006904No Hit
GAATTAGGACTCCTCAGGTCACCTTCTCACAATGAGGCTCCTTGCTCAGCTTCTGGGGCTGCTAATGCTCTGGGT11890.14402519532432922No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT11420.13833202107685785No Hit
GCCTACATGGAGCTGAGCAGACTAACATCTGACGACACGGCCGTCTATTACTGTGCGAGTCCCTCAGGGTCCTTT11280.13663618193931318No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG11050.13385016049906123No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTC10940.13251771546241897No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTAGGTGGGAAGTTTCTGGCGGTCACGTTCTGTCCGCTTTCGCTCCAG10880.13179092726061414No Hit
GTGTGACCTGGAGCGAAAGCGGACAGAACGTGACCGCCAGAAACTTCCCACCTAGCCAGGATGCCTCCGGGGAC10810.1309430076918418No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT10410.12609775301314277No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAA10310.12488643934346799No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGA10010.12125249833444371No Hit
CCCTAAAAGTGGTGACACAGACTATACACAGAAATTTCAGGGCAGGGTCACCATGACCAGGGACACGTCCATCA9930.12028344739870389No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCC9810.11882987099509418No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT9770.11834534552722428No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGACTTGCCGTCTGGGCACTGTGTGGCC9760.1182242141602568No Hit
GGCCAAAACTGTACGGAAATTGTGTAGCTTGCATGCAGTAATAAACTCCGACATCTTCAGCTTCCACCCTGCT9760.1182242141602568No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC9590.11616498092180971No Hit
GTGCAGCGACAGTCGGGGGTGGCAGCATGGGGGAGGTGGGGGAACTGGGCAGGGCACAGTCACATCCTGGCTGG9220.11168312034401308No Hit
GTCGCTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC9140.11071406940827326No Hit
GGTCTAGTCAGAGCCTCGTACACGCTGATGGAAACACCTACTTGAGTTGGCATCAGCTGAGGCCAGGCCAGCCTC9000.1090182302707286No Hit
GTCTGGGACCCCAGAGAACCGGTTAGAAGTCTTATAAATTAGGAGTCTTGGAGGCTGGCCTGGCCTCAGCTGAT8990.10889709890376112No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC8980.10877596753679365No Hit
GGGTGCGGCAGGCCCCTGGACAAGGGCTTGAGTGGATGGGACGGATCCACCCTAAAAGTGGTGACACAGACTATA8950.10841257343589122No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACA8920.1080491793349888No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT8780.10635334019744414No Hit
CTCCTCACCTGTCACCCTTGGACAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAGAGCCTCGTACACGCTGATG8600.10417297559202955No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCTGGTGCCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGC8590.10405184422506207No Hit
CCCCTGGACAAGGGCTTGAGTGGATGGGACGGATCCACCCTAAAAGTGGT8560.10368845012415964No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCATGCG8530.10332505602325723No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAACTTGGTCCCCTGGCCAAAACTG8520.10320392465628975No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT8420.10199261098661498No Hit
GTATCAACGCAGAGTACGGGAATTAGGACTCCTCAGGTCACCTTCTCACAATGAGGCTCCTTGCTCAGCTTCTGG8380.10150808551874509No Hit
GTCCCAGACAGATTCAGTGGCAGTGGGGCAGGGACAGATTTCACACTGAAAATCAGCAGGGTGGAAGCTGAAGAT8360.10126582278481014No Hit
CCTCAGGTCACCTTCTCACAATGAGGCTCCTTGCTCAGCTTCTGGGGCTGCTAATGCTCTGGGTCCCTGGATCCA8280.10029677184907032No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGTAT651.515475E-597.6760670
AATGGTA3100.092.16209470
GTAACCA650.001854983773.2570570
CTCGCAA201.0150544E-466.5907221
CCGCTAT1051.6324224E-460.46613770
TCGGTTT850.005370272356.02009670
GTACGTA656.7498513E-753.28205569
ACTGTCA900.0067328652.90786770
CGGTAAT200.0079097450.81252736
ATGGCAA1901.0251206E-650.1232470
TACCACA2255.7963916E-849.3806870
AGAGCAT6250.045.712470
GCGGTAA306.964138E-445.0887535
TACGATG307.587851E-444.3104637
GCTAATA1458.0285233E-443.78582470
GTAGTTT1458.0285233E-443.78582470
GAGCAAA12500.043.1728270
GCCGGTT3407.4578566E-1042.0150770
AACGAAG406.236011E-541.5410586
GGTATCA8250.039.5316621
Produced by FastQC (version 0.11.5)