FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004685226

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685226
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1024083
Sequences flagged as poor quality0
Sequence length18-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTAAGTCTGTTGACAGAAGTAAAATCCACAATCCTCCGGTTGCAGACCGGTGATGGTGAGAGTGAAATCTGTC47760.46636844865113475No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTAT44630.4358045197508405No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC43420.4239890711983306No Hit
GACTTACACTATCTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCT38490.3758484419719886No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC33350.3256571977076077No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCT31840.31091229910075646No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGG28530.27859070016785753No Hit
ATCTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCG28450.2778095134866998No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG27460.2681423283073735No Hit
GATCTATGCTGCATCCAATTTGCAAAGTGGGGTCCCATCAAGGTTCAGTGGCAGTAGATCTGGGACAGATTTCA27060.2642363949015851No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGT26560.25935397814434963No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA26560.25935397814434963No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC25580.24978444130016805No Hit
CACCATCACCGGTCTGCAACCGGAGGATTGTGGATTTTACTTCTGTCAACAGACTTACACTATCTGGACGTTCGG24950.24363259618605132No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCC24450.23875017942881585No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG24420.2384572344233817No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG23570.23015712593608137No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG22000.2148263373183619No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT21470.20965097555569223No Hit
GCATAGATCAGGAGGTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAATTTAATTGATTGCTAATGGTCTGACT21210.2071121188419298No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG20690.2020344054144049No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTC20090.1961755053057223No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT19690.19226957189993388No Hit
GTCTCCATCCTCCCTGTCTGCTTCTATAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGACCATTAGCAA19670.1920742752296445No Hit
GTGATGGTGAGAGTGAAATCTGTCCCAGATCTACTGCCACTGAACCTTGATGGGACCCCACTTTGCAAATTGGA19460.19002366019160558No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACT18920.18475065009379124No Hit
CGTCCAGATAGTGTAAGTCTGTTGACAGAAGTAAAATCCACAATCCTCCGGTTGCAGACCGGTGATGGTGAGAG18740.18299298006118644No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAAC18640.18201649670973935No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT18590.1815282550340158No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC18170.177427024957938No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCT17650.17234931153041305No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGCGTCATACAGATGACCCAGTC17310.16902926813549293No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCC17170.16766219144346697No Hit
GCGTCATACAGATGACCCAGTCTCCATCCTCCCTGTCTGCTTCTATAGGAGACAGAGTCACCATCACTTGCCGGG17160.16756454310832228No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG16750.16356096136738918No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT16440.16053386297790317No Hit
GATTTCACTCTCACCATCACCGGTCTGCAACCGGAGGATTGTGGATTTTACTTCTGTCAACAGACTTACACTA16000.15623733623153593No Hit
GATCAGGAGGTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAATTTAATT15850.15477261120436528No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC15300.14940195277140622No Hit
GGTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAATTTAATTGATTGCTAATGGTCTGACTTGCCCGGCAAGTGA14710.14364070099786835No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG14230.13895358091092228No Hit
GAGTGAAATCTGTCCCAGATCTACTGCCACTGAACCTTGATGGGACCCCACTTTGCAAATTGGATGCAGCATAGA14120.13787944922433046No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT14100.137684152554041No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA14090.13758650421889632No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13960.1363170758620151No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC13610.13289938413195024No Hit
ATAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGACCATTAGCAATCAATTAAATTGGTATCAGCAGAAA13330.13016523074789837No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA13040.1273334290287018No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC12700.12401338563378164No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCA12630.12332984728776868No Hit
CAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCT12000.11717800217365193No Hit
CTATAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGACCATTAGC11950.1166897604979284No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTT11760.11483444213017889No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA11760.11483444213017889No Hit
GCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGCGTCATACAGATGACCCAGTCTCCATCCTCC11740.11463914545988949No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTG11730.11454149712474476No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA11590.11317442043271883No Hit
CTATCTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCC11430.11161204707040348No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC11370.11102615705953522No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTT11350.11083086038924579No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC11180.10917083869178572No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC11060.1079990586700492No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC10940.10682727864831268No Hit
GTGGGGTCCCATCAAGGTTCAGTGGCAGTAGATCTGGGACAGATTTCACT10870.1061437403022997No Hit
GGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTTCATC10680.10428842193455022No Hit
GTCTGCAACCGGAGGATTGTGGATTTTACTTCTGTCAACAGACTTACACT10660.10409312526426082No Hit
GCCCCTAACCTCCTGATCTATGCTGCATCCAATTTGCAAAGTGGGGTCCCATCAAGGTTCAGTGGCAGTAGA10620.10370253192368198No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT10580.10331193858310313No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC10540.10292134524252429No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC10330.10087073020448536No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA10280.10038248852876183No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA10280.10038248852876183No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)