FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685230

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685230
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences89450
Sequences flagged as poor quality0
Sequence length18-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTAT5380.6014533258803801No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC4400.49189491335941865No Hit
GTGTTAGTTCCAACAATAAGAACTTCTTAGTTTGGTTCCAGCAGAAATCAGGACAGCCTCCTAAGCTGCTCATT4040.45164896590273895No Hit
CTCCTAAGCTGCTCATTTCCTGGGCATCTACCCGGGAATCCGGGGTCCCTGACCGATTCAGTGGCAGCGGGTC4000.4471771939631079No Hit
GCTTAGGAGGCTGTCCTGATTTCTGCTGGAACCAAACTAAGAAGTTCTTATTGTTGGAACTAACACTCTGGC3720.4158747903856903No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCT3680.4114030184460593No Hit
CTCCATCAGCAACCTGCAGGCTGAAGATGCGGCAGTTTATTATTGTCAGCAATTTTATCGTACTCCATTGACTT3070.3432084963666853No Hit
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGT3040.339854667411962No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG2950.32979318054779205No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCC2820.31525992174399103No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC2730.3051984348798211No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG2610.2917831190609279No Hit
GATTTCACTCTCTCCATCAGCAACCTGCAGGCTGAAGATGCGGCAGTTTATTATTGTCAGCAATTTTATCGTAC2500.2794857462269424No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAAC2440.2727780883174958No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT2390.267188373392957No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG2320.25936277249860257No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT2290.25600894354387926No Hit
ATTCAGTGGCAGCGGGTCTGGAACAGATTTCACTCTCTCCATCAGCAACCTGCAGGCTGAAGATGCGGCAGTTTA2130.23812185578535494No Hit
GTGTTGCAGACCCAGGTCTCCATTTCTCTGTTGCTCTGGATCTCTGGTGCCTACGGGGACATCGTGATGACCCAG2120.23700391280044716No Hit
CTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCA2020.22582448295136948No Hit
GTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTGTTAGTTC1990.22247065399664617No Hit
CCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGC1970.22023476802683065No Hit
GTCCCTGACCGATTCAGTGGCAGCGGGTCTGGAACAGATTTCACTCTCTCCATCAGCAACCTGCAGGCTGAAGA1960.21911682504192287No Hit
GTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTGTTAGTTCCAACAATAAGAACTTCTTAGT1950.21799888205701506No Hit
GTTTATTATTGTCAGCAATTTTATCGTACTCCATTGACTTTCGGCCCTGGGACCACAGTGGATATCAAACGAA1940.21688093907210734No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACT1860.20793739519284515No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA1730.19340413638904416No Hit
GAGTGTTAGTTCCAACAATAAGAACTTCTTAGTTTGGTTCCAGCAGAAATCAGGACAGCCTCCTAAGCTGCTCAT1730.19340413638904416No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT1700.19005030743432086No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATATCCACTGTGGTCC1660.18557853549468978No Hit
AATCAGGACAGCCTCCTAAGCTGCTCATTTCCTGGGCATCTACCCGGGAATCCGGGGTCCCTGACCGATTCAG1640.18334264952487425No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA1600.17887087758524314No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA1590.1777529346003354No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC1570.17551704863051984No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCA1510.16880939072107323No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC1510.16880939072107323No Hit
GCTCATTTCCTGGGCATCTACCCGGGAATCCGGGGTCCCTGACCGATTCAGTGGCAGCGGGTCTGGAACAGATT1500.16769144773616546No Hit
GTCAGGGACCCCGGATTCCCGGGTAGATGCCCAGGAAATGAGCAGCTTAGGAGGCTGTCCTGATTTCTGC1490.16657350475125768No Hit
CCATTGACTTTCGGCCCTGGGACCACAGTGGATATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCG1470.16433761878144215No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC1420.1587479038569033No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA1420.1587479038569033No Hit
CAATAAGAACTTCTTAGTTTGGTTCCAGCAGAAATCAGGACAGCCTCCTAAGCTGCTCATTTCCTGGGCATCTAC1390.15539407490218No Hit
GTGATATACGGCTGTGTCTTCGCTTTTCAGGCTGCTCATTTGCAGATACA1390.15539407490218No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC1370.15315818893236444No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC1320.1475684740078256No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGG1320.1475684740078256No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG1280.14309670206819453No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC1280.14309670206819453No Hit
GACCGATTCAGTGGCAGCGGGTCTGGAACAGATTTCACTCTCTCCATCAG1270.14197875908328675No Hit
GGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTCCATTTCTCTGTT1270.14197875908328675No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT1260.140860816098379No Hit
GTCTGGAACAGATTTCACTCTCTCCATCAGCAACCTGCAGGCTGAAGATGCGGCAGTTTATTATTGTCAGCAATT1260.140860816098379No Hit
GGCTGAAGATGCGGCAGTTTATTATTGTCAGCAATTTTATCGTACTCCATTGACTTTCGGCCCTGGGACCACAG1240.13862493012856344No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC1230.1375069871436557No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGT1210.13527110117384014No Hit
CCATTTCTCTGTTGCTCTGGATCTCTGGTGCCTACGGGGACATCGTGATGACCCAGTCTCCAGACTCCC1190.1330352152040246No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTT1190.1330352152040246No Hit
GTGCAGCCACAGTTCGTTTGATATCCACTGTGGTCCCAGGGCCGAAAGTC1180.1319172722191168No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG1170.13079932923420906No Hit
ACCCGGGAATCCGGGGTCCCTGACCGATTCAGTGGCAGCGGGTCTGGAACAGATTTCACTCTCTCCATCAGCAAC1170.13079932923420906No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC1140.12744550027948576No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA1130.12632755729457798No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA1130.12632755729457798No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA1120.1252096143096702No Hit
ATTTTATCGTACTCCATTGACTTTCGGCCCTGGGACCACAGTGGATATCAAACGAACTGTGGCTGCACCA1120.1252096143096702No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA1120.1252096143096702No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG1110.12409167132476243No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC1110.12409167132476243No Hit
CTACGGGGACATCGTGATGACCCAGTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAAC1100.12297372833985466No Hit
GTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA1090.1218557853549469No Hit
GTCCTGATTTCTGCTGGAACCAAACTAAGAAGTTCTTATTGTTGGAACTAACACTCTGGCTGGACTTGCAGTTGA1090.1218557853549469No Hit
CTCTCCATCAGCAACCTGCAGGCTGAAGATGCGGCAGTTTATTATTGTCAGCAATTTTATCGTACTCCATTGACT1080.12073784237003912No Hit
TTATTATTGTCAGCAATTTTATCGTACTCCATTGACTTTCGGCCCTGGGACCACAGTGGATATCAAACGAACTG1080.12073784237003912No Hit
GGATCTCTGGTGCCTACGGGGACATCGTGATGACCCAGTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGG1050.11738401341531582No Hit
GGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTGTTAGTTCCAACAATAAGAACT1040.11626607043040806No Hit
GGTCTGGAACAGATTTCACTCTCTCCATCAGCAACCTGCAGGCTGAAGATGCGGCAGTTTATTATTGTCAGCAAT1040.11626607043040806No Hit
CCTGCAGGCTGAAGATGCGGCAGTTTATTATTGTCAGCAATTTTATCGTACTCCATTGACTTTCGGCCCTGGGAC1040.11626607043040806No Hit
CTCCTGTGCAGTCTCTGGACTCACTTTCAGTGACGCCTGGTTGAGCTGGGTCCGCCAGGCTCCAGGGAAGGGG1020.11403018446059252No Hit
AGTCCAGCCAGAGTGTTAGTTCCAACAATAAGAACTTCTTAGTTTGGTTC1020.11403018446059252No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG1000.11179429849077697No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTT1000.11179429849077697No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT980.10955841252096143No Hit
CTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGC980.10955841252096143No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG950.10620458356623813No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC950.10620458356623813No Hit
GTATCAACGCAGAGTACGGGGAGCTACAACAGGCAGGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTCCA930.10396869759642258No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC930.10396869759642258No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC930.10396869759642258No Hit
ACAACAGGCAGGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTCCATTTCTCTGTTGCTCTGGATCTCTGG920.10285075461151483No Hit
ACAGTGGATATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAA920.10285075461151483No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT910.10173281162660704No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT900.10061486864169927No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTG900.10061486864169927No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTACTG150.001014075485.2415768
GTACTGT200.001151237582.5367169
AATGGTA804.643516E-878.0771970
TAGGTAC150.00189651372.81583466
TGTTAGG150.00212276670.7778463
TTGTTAG150.002172326770.3675462
GGTTTGT150.002243154469.8010359
TCGATTG250.002790380366.0293869
TCTTCGG500.003460975862.4617570
ACTCGAT200.004989871357.0413567
ATGTTCA300.00574444355.0244869
TACTCGA200.005928685454.61187466
GTTAGGT200.006482076453.3947264
TTTGTTA200.00683516752.68402561
CGTTCCC200.007009004252.35077758
TGTGGGT200.007073026652.23063355
ATCGGGA200.007073026652.23063355
GGGTCCG803.3756386E-452.05145670
AAAGGGG600.007122208452.05145670
TGCACAG600.007122208452.05145670
Produced by FastQC (version 0.11.5)