FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685231

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685231
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences89450
Sequences flagged as poor quality0
Sequence length18-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6890.7702627166014533No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT5740.6416992733370599No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTC3200.3577417551704863No Hit
CTCCTAAGCTGCTCATTTCCTGGGCATCTACCCGGGAATCCGGGGTCCCTGACCGATTCAGTGGCAGCGGGTCTG3190.35662381218557854No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT3010.3365008384572387No Hit
CTCCATCAGCAACCTGCAGGCTGAAGATGCGGCAGTTTATTATTGTCAGCAATTTTATCGTACTCCATTGACTTT3000.3353828954723309No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTA2860.31973169368362214No Hit
GCTTAGGAGGCTGTCCTGATTTCTGCTGGAACCAAACTAAGAAGTTCTTATTGTTGGAACTAACACTCTGGCTG2790.31190609278926773No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC2610.2917831190609279No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTT2540.2839575181665735No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACT2440.2727780883174958No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCC2420.2705422023476803No Hit
GTGTTAGTTCCAACAATAAGAACTTCTTAGTTTGGTTCCAGCAGAAATCAGGACAGCCTCCTAAGCTGCTCATTT2240.2504192286193404No Hit
GTGCAGCCACAGTTCGTTTGATATCCACTGTGGTCCCAGGGCCGAAAGTC2230.24930128563443263No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT2210.2470653996646171No Hit
GATTTCACTCTCTCCATCAGCAACCTGCAGGCTGAAGATGCGGCAGTTTATTATTGTCAGCAATTTTATCGTACT2190.24482951369480155No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT2170.24259362772498602No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATATCCACTGTGGTCCCAGGGCCGAAAGTCAATGGAGTAC2140.23923979877026272No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC1920.21464505310229176No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT1920.21464505310229176No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG1890.21129122414756846No Hit
GTTTATTATTGTCAGCAATTTTATCGTACTCCATTGACTTTCGGCCCTGGGACCACAGTGGATATCAAACGAA1870.20905533817775296No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGC1790.20011179429849077No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA1750.1956400223588597No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT1730.19340413638904416No Hit
ATTCAGTGGCAGCGGGTCTGGAACAGATTTCACTCTCTCCATCAGCAACC1720.19228619340413639No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGTTA1670.18669647847959756No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA1660.18557853549468978No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACA1600.17887087758524314No Hit
GTCCCTGACCGATTCAGTGGCAGCGGGTCTGGAACAGATTTCACTCTCTCCATCAGCAACCTGCAGGCTGAAGAT1560.17439910564561206No Hit
ATAATAAACTGCCGCATCTTCAGCCTGCAGGTTGCTGATGGAGAGAGTGAAATCTGTTCCAGACCCGCTGCCA1560.17439910564561206No Hit
CCATTGACTTTCGGCCCTGGGACCACAGTGGATATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCC1530.17104527669088876No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCA1510.16880939072107323No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA1480.16545556176634993No Hit
GTGTTGCAGACCCAGGTCTCCATTTCTCTGTTGCTCTGGATCTCTGGTGC1450.16210173281162663No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAG1430.15986584684181107No Hit
GAGTGAAATCTGTTCCAGACCCGCTGCCACTGAATCGGTCAGGGACCCCG1430.15986584684181107No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGA1410.15762996087199552No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGG1370.15315818893236444No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT1350.1509223029625489No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA1350.1509223029625489No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATATCCACTGTG1350.1509223029625489No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGAT1330.14868641699273338No Hit
CCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGC1330.14868641699273338No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAA1320.1475684740078256No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG1300.14533258803801005No Hit
GTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTGTTAGTTC1300.14533258803801005No Hit
CTCCTGTGCAGTCTCTGGACTCACTTTCAGTGACGCCTGGTTGAGCTGGGTCCGCCAGGCTCCAGGGAAGGG1270.14197875908328675No Hit
GAGTGTTAGTTCCAACAATAAGAACTTCTTAGTTTGGTTCCAGCAGAAAT1210.13527110117384014No Hit
CTGATGGAGAGAGTGAAATCTGTTCCAGACCCGCTGCCACTGAATCGGTCAGGGACCCCGGATTCCCGGGTAGAT1190.1330352152040246No Hit
CTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTGTTAGTTCCAACAATAA1180.1319172722191168No Hit
ATTTTATCGTACTCCATTGACTTTCGGCCCTGGGACCACAGTGGATATCAAACGAACTGTGGCTGCACCAT1180.1319172722191168No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA1170.13079932923420906No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT1160.12968138624930128No Hit
GTCTGGAACAGATTTCACTCTCTCCATCAGCAACCTGCAGGCTGAAGATG1140.12744550027948576No Hit
AATCAGGACAGCCTCCTAAGCTGCTCATTTCCTGGGCATCTACCCGGGAATCCGGGGTCCCTGACCGATTCAGTG1130.12632755729457798No Hit
GTGGATATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCC1120.1252096143096702No Hit
CAATAATAAACTGCCGCATCTTCAGCCTGCAGGTTGCTGATGGAGAGAGTGAAATCTGTTCCAGACCCGCTGCCA1120.1252096143096702No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGG1110.12409167132476243No Hit
CTGTGGTCCCAGGGCCGAAAGTCAATGGAGTACGATAAAATTGCTGACAATAATAAACTGCCGCATCTTCAGC1110.12409167132476243No Hit
CCTGCAGGCTGAAGATGCGGCAGTTTATTATTGTCAGCAATTTTATCGTACTCCATTGACTTTCGGCCCTGGGAC1100.12297372833985466No Hit
AGTCAATGGAGTACGATAAAATTGCTGACAATAATAAACTGCCGCATCTTCAGCCTGCAGGTTGCTGATGGAGA1100.12297372833985466No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTG1080.12073784237003912No Hit
GTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTGTTA1080.12073784237003912No Hit
GTCAGGGACCCCGGATTCCCGGGTAGATGCCCAGGAAATGAGCAGCTTAGGAGGCTGTCCTGATTTCTGCTGGAA1070.11961989938513136No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAA1070.11961989938513136No Hit
CAATAAGAACTTCTTAGTTTGGTTCCAGCAGAAATCAGGACAGCCTCCTAAGCTGCTCATTTCCTGGGCATCTAC1060.11850195640022358No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC1060.11850195640022358No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT1050.11738401341531582No Hit
GTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATA1050.11738401341531582No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGT1050.11738401341531582No Hit
GTGATATACGGCTGTGTCTTCGCTTTTCAGGCTGCTCATTTGCAGATACATGGTGTCTTTTAAATCATCTCTTGA1030.11514812744550028No Hit
TCTTCAGCCTGCAGGTTGCTGATGGAGAGAGTGAAATCTGTTCCAGACCCGCTGCCACTGAATCGGTCAGGGACC1020.11403018446059252No Hit
GATATCCACTGTGGTCCCAGGGCCGAAAGTCAATGGAGTACGATAAAATTGCTGACAATAATAAACTGCCGCAT1010.11291224147568474No Hit
GTATCAACGCAGAGTACGGGGAGCTACAACAGGCAGGCAGGGGCAGCAAG1010.11291224147568474No Hit
GGCTGAAGATGCGGCAGTTTATTATTGTCAGCAATTTTATCGTACTCCATTGACTTTCGGCCCTGGGACCACAGT1010.11291224147568474No Hit
TTATTATTGTCAGCAATTTTATCGTACTCCATTGACTTTCGGCCCTGGGACCACAGTGGATATCAAACGAACTGT1010.11291224147568474No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT1000.11179429849077697No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC990.11067635550586918No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTA960.10732252655114588No Hit
CCACAGTGGATATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAAT960.10732252655114588No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA960.10732252655114588No Hit
GACCGATTCAGTGGCAGCGGGTCTGGAACAGATTTCACTCTCTCCATCAGCAACCTGCAGGCTGAAGATGCGGCA950.10620458356623813No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA940.10508664058133034No Hit
ACAGTGGATATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAAT930.10396869759642258No Hit
CTCTCCATCAGCAACCTGCAGGCTGAAGATGCGGCAGTTTATTATTGTCA900.10061486864169927No Hit
GCTCATTTCCTGGGCATCTACCCGGGAATCCGGGGTCCCTGACCGATTCAGTGGCAGCGGGTCTGGAACAGATTT900.10061486864169927No Hit
GGTCTGGAACAGATTTCACTCTCTCCATCAGCAACCTGCAGGCTGAAGAT900.10061486864169927No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGTAA109.904555E-4279.2972770
TTTAAGT500.001075483483.7891770
TCTTGTT951.8378641E-458.7994270
CGCGGTG200.0078147650.93067627
TGACACA200.00783209550.9021625
GGCTCCC200.00783209550.9021626
GGCACCG200.00788427250.81689
ACCGCCT200.00788427250.816812
GCACCGC200.00788427250.816810
AGTTTAT2452.9331022E-739.89960570
AAACACA1809.039793E-538.79128370
GTATCAA2650.038.373751
GGTATCA1100.036.978341
TCAACGC3050.034.433244
CAACGCA3100.033.877875
ATCAACG3100.033.877873
AACGCAG3100.033.877876
TATCAAC3100.032.7850342
ATAAGAA1750.00371907531.91968570
ACGCAGA3300.031.824667
Produced by FastQC (version 0.11.5)