FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004685238

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685238
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1051315
Sequences flagged as poor quality0
Sequence length18-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTAT43030.4092969281328622No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC40980.38979753927224475No Hit
GTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA33510.3187436686435559No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCT30830.2932517846696756No Hit
GTAATACACGGCCGTGTCCGCGGCGGTCACAGAGGTCAAGTTCAGGGAGAACTGGTTCTTGGACGTGTCTACTGA29430.27993512886242466No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG28540.2714695405278152No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC25930.24664348934429736No Hit
GTCTTTGTCTCCAGGGGAAGGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTACCACCTACTTAGCCTGGTA25660.244075277152899No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC25410.24169730290160418No Hit
GTCTCCAGCCACCCTGTCTTTGTCTCCAGGGGAAGGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTACCAC24610.2340877852974608No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG24170.22990255061518194No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG23970.22800017121414612No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT23790.22628802975321383No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAAC23630.22476612623238518No Hit
TTATTATTGTCAGCAGCGTGGCAGCTGGCCTCGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTG23420.2227686278612975No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCC22950.21829803626886327No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTACCACCTACTTAGCCTGGTACCAACAGAAACCTGGCCAGGCTCCCAGGC22740.21630053789777562No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGCCTAGAGCCTGAAGATTCTGCACTTTATTATTGTCAGCAGCG22250.21163970836523782No Hit
GTCTCTGGTGGCTCCATCAATGGTTACTACTGGAACTGGATCCGGCAGACCGCCGGGAAGGGACCGGAGTACAT21750.20688375986264823No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA21700.20640816501238923No Hit
GTCAGAGTGTTACCACCTACTTAGCCTGGTACCAACAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGA21340.20298388209052473No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG21040.20013031298897094No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT21020.19994007504886735No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACT20250.19261591435487938No Hit
GAGTGTTACCACCTACTTAGCCTGGTACCAACAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGATGCATC20170.19185496259446502No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGA19600.18643318130151287No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTCCGAGGCCAG19570.1861478243913575No Hit
GTGTTACCACCTACTTAGCCTGGTACCAACAGAAACCTGGCCAGGCTCCC19500.18548199160099496No Hit
CAATAATAAAGTGCAGAATCTTCAGGCTCTAGGCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCG19020.1809162810385089No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTC18600.17692128429633364No Hit
GTGTTGACACAGTCTCCAGCCACCCTGTCTTTGTCTCCAGGGGAAGGAGC18510.17606521356586752No Hit
GACTTCACTCTCACCATCAGCAGCCTAGAGCCTGAAGATTCTGCACTTTATTATTGTCAGCAGCGTGGCAGC18330.17435307210493525No Hit
GCCTAGAGCCTGAAGATTCTGCACTTTATTATTGTCAGCAGCGTGGCAGCTGGCCTCGGACGTTCGGCCAAGGGA17950.17073855124296713No Hit
GTCCCAGACCCACTGCCGCTGAACCTGGCTGGGATGCCAGTGGCCCTGTTGGATGCATCATAGATGAGGAGCC17030.16198760599820225No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCGCTGAACCTGGCTGGGATGCCAGTGGCCCTGTTGGATGCATCATAGA16990.16160713011799507No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT16400.15599511088493934No Hit
GTGTATTACTGTGCGAGAGATACCCCTAGCGATTCGGGGACTTATTATGTTGACTACTGGGGCCAGGGAACCC16260.15466344530421425No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT16250.15456832633416245No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA15620.14857583122089954No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC15470.14714904667012266No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCA15310.14562714314929398No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA14510.1380176255451506No Hit
TCTATGATGCATCCAACAGGGCCACTGGCATCCCAGCCAGGTTCAGCGGCAGTGGGTCTGGGACAGACTTCAC14470.13763714966494342No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG14330.13630548408421836No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC14320.13621036511416654No Hit
ACAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGATGCATCCAACAGGGCCACTGGCATCCCAGCCAGGT14270.1357347702639076No Hit
CTGCTACTCTGGCTCCCAGATACCACCGGAGAAGTTGTGTTGACACAGTC14140.13449822365323427No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC14100.13411774777302712No Hit
CTTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGT13760.13088370279126618No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG13730.1305983458811108No Hit
CAGTAATACACGGCCGTGTCCGCGGCGGTCACAGAGGTCAAGTTCAGGGAGAACTGGTTCTTGGACGTGTCTA13490.1283154905998678No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC13380.12726918192929806No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC13340.1268887060490909No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAC13240.12593751634857298No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAGTTGAGGGAGATGGGGTAGGTGGAGT12940.12308394724701921No Hit
TCATAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGTTGGTACCAGGCTAAGTAGGTGGTAACACTCTGAC12930.12298882827696742No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTG12590.11975478329520647No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT12420.118137760804326No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA12370.11766216595406705No Hit
CTCATGGACCTCCTGCACAAGAACATGAAACACCTGTGGTTCTTCCTCCTGCTGGTGGCAGCTCCCAGATGGGTC12280.11680609522360093No Hit
CCCAGATACCACCGGAGAAGTTGTGTTGACACAGTCTCCAGCCACCCTGTCTTTGTCTCCAGGGGAAGGAGCCAC12230.11633050037334194No Hit
CATCTATGATGCATCCAACAGGGCCACTGGCATCCCAGCCAGGTTCAGCGGCAGTGGGTCTGGGACAGACTTCA12080.11490371582256508No Hit
GAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTACCACCTACTTAGCCTGGTACCAACAGAAACCTGGCCAGG12020.11433300200225432No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG11990.11404764509209894No Hit
GGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTTCATC11910.1132866933316846No Hit
CACTCTGACTGGCCCTGCAGGAGAGGGTGGCTCCTTCCCCTGGAGACAAA11750.11176478981085594No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTT11680.11109895702049338No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA11450.10891122070930218No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC11440.10881610173925037No Hit
GCCTGGGAGCCTGGCCAGGTTTCTGTTGGTACCAGGCTAAGTAGGTGGTA11400.10843562585904319No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA11390.10834050688899141No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCT11350.10796003100878424No Hit
GTATTACTGTGCGAGAGATACCCCTAGCGATTCGGGGACTTATTATGTTGACTACTGGGGCCAGGGAACCCTGG11310.10757955512857707No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA11220.10672348439811094No Hit
CAACAGGGCCACTGGCATCCCAGCCAGGTTCAGCGGCAGTGGGTCTGGGACAGACTTCACTCTCACCATCAGCAG11130.10586741366764481No Hit
CCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCACCGGAGAAGTTGTGTTGACACAGTCTC11100.10558205675748943No Hit
CTCTTCCTCCTGCTACTCTGGCTCCCAGATACCACCGGAGAAGTTGTGTTGACACAGTCTCCAGCCACCCTGTCT11070.10529669984733404No Hit
CTCCCAGGCTCCTCATCTATGATGCATCCAACAGGGCCACTGGCATCCCAGCCAGGTTCAGCGGCAGTGGGTC11020.10482110499707509No Hit
ACCCTCTCCTGCAGGGCCAGTCAGAGTGTTACCACCTACTTAGCCTGGTA10930.10396503426660895No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC10860.10329920147624642No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT10830.10301384456609104No Hit
CACCTGCACTGTCTCTGGTGGCTCCATCAATGGTTACTACTGGAACTGGA10770.10244313074578028No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC10770.10244313074578028No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC10690.10168217898536595No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC10680.10158706001531415No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA10650.10130170310515876No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC10580.10063587031479623No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA10570.10054075134474444No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC10550.10035051340464085No Hit
CTGCAGGAGAGGGTGGCTCCTTCCCCTGGAGACAAAGACAGGGTGGCTGG10520.10006515649448547No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)