FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685242

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685242
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences683143
Sequences flagged as poor quality0
Sequence length18-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAAACAGCCGTGTCGTCGGCGCTCAGGCTGTTCATTTGCAGATACA24460.3580509498011397No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCTGGGGAGCGGGGGCTTGCTGGCCG24010.3514637491711106No Hit
GAGTTACACTTCCCCGATCACCTTCGGCCAAGGGACACGACTGGACATTAAACGAACTGTGGCTGCACCA22700.3322876762259146No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC22330.32687153348566844No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTAT20380.2983269974222088No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGTTTTGGAGATGGTTTTCTCGATGGGGGCTGGGAGGCCTTTG18740.2743203106816582No Hit
GTGTAACTCTGTTGACAGTAGTAGGTTGCAAAATCTTCAGGTTGCAGACCGCTGATGGTGAGAGTGAAATCTGTC16580.24270174765751826No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCT15310.22411120365721382No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGAGCGCCTGAGT14830.21708485631851604No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC14600.21371806488539002No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCT14380.2104976556884869No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC14370.21035127345226404No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG14310.20947298003492681No Hit
GATCTATGCTGCATCCAATTTGCAAAGTGGGGTCCCGTCAAGGTTCAGTGGCAGTGGATTTGGGACAGATTTCA14130.20683809978291517No Hit
GTTTATTACTGTGTGAGAGAGACTAAATATACGACTCATAATGCTTTTGA13830.20244663269622906No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG13460.19703048995598285No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG13300.19468837417641693No Hit
GAGTACAAGTGCAAGGTCTCCAACAAAGGCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAACCAAAGGGCAG13160.19263902286929677No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGG12920.1891258491999479No Hit
ACCTACTACTGTCAACAGAGTTACACTTCCCCGATCACCTTCGGCCAAGGGACACGACTGGACATTAAACGAA12840.18795479131016493No Hit
GGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCTGGGGAGCGGGGGCTTGCTGGCCGTGGCACTCATTTA12820.18766202683771918No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG12730.18634458671171336No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA12520.1832705597510331No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGACCTCCAGCAACTTCGGCACCCAGAC11670.17082806967208913No Hit
GTGGTGGACGTGAGCCACGAAGACCCCGAGGTCCAGTTCAACTGGTACGT11040.16160598879004837No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCC10990.160874077608934No Hit
GCATGGAGGTGCATAATGCCAAGACAAAGCCACGGGAGGAGCAGTTCAACAGCACGTTCCGTGTGGTCAGCGTC10970.16058131313648824No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT10930.1599957841915968No Hit
GGACACGACTGGACATTAAACGAACTGTGGCTGCACCATCTGTCTTCATC10820.15838557959314523No Hit
GTATGTGGTAATACCACTACCAGTAATGTAGGAAACCCACTCCAGCCCCTTCCCTGGAGCCTGGCGGACCCAGT10810.15823919735692232No Hit
GTAGTAGGTTGCAAAATCTTCAGGTTGCAGACCGCTGATGGTGAGAGTGAAATCTGTCCCAAATCCACTGCCA10730.15706813946713938No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACT10600.15516517039624206No Hit
GAGTTGGGGCTGTGCTGGGTTTTCCTTGTTGCTATTTTAGAAGGTGTCCAGTGTGAGGTGCAGCTGGTGGAG10560.15457964145135059No Hit
CTTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGT10090.1476996763488757No Hit
GCATAGATCAGGAGCTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAATTTAAATAAGTGTAAATGGTCTGACT10080.14755329411265283No Hit
GATTTCACTCTCACCATCAGCGGTCTGCAACCTGAAGATTTTGCAACCTACTACTGTCAACAGAGTTACACTTCC9890.14477203162441832No Hit
GCCCAGGGCCGCTGTGCTCTCGGAGGTGCTCCTGGAGCAGGGCGCCAGGG9830.1438937382070811No Hit
GCACTGAACACAGAGGACTCACCATGGAGTTGGGGCTGTGCTGGGTTTTC9500.13906312441172639No Hit
CTACTACTGTCAACAGAGTTACACTTCCCCGATCACCTTCGGCCAAGGGACACGACTGGACATTAAACGAACTG9440.13818483099438916No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT9420.13789206652194344No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAAC9180.13437889285259455No Hit
CATTTACCCGGAGACAGGGAGAGGCTCTTCTGTGTGTAGTGGTTGTGCAG9180.13437889285259455No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG9070.13276868825414298No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTAATGTCCAGTCGTGTCCCTTGGCCGAAGGTGATCGGGGAAG8770.12837722116745687No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC8700.1273525455138968No Hit
CAACTGTCTTGTCCACCTTGGTGTTGCTGGGCTTGTGATCTACGTTGCAGGTGTAGGTCTGGGTGCCGAAGT8570.12544957644299948No Hit
GTCGTGTCCCTTGGCCGAAGGTGATCGGGGAAGTGTAACTCTGTTGACAGTAGTAGGTTGCAAAATCTTCAGGT8560.12530319420677663No Hit
GACCTACACCTGCAACGTAGATCACAAGCCCAGCAACACCAAGGTGGACAAGACAGTTGAGCGCAAATGTTGTG8460.12383937184454794No Hit
GTCTCCATCCTCCCTGTCTGCATCTGAAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGACCATTTACA8460.12383937184454794No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT8260.12091172712009053No Hit
GTGTAGTGGTTGTGCAGAGCCTCATGCATCACGGAGCATGAGAAGACGTT8200.1200334337027533No Hit
GTCTTCCCCCTGGCGCCCTGCTCCAGGAGCACCTCCGAGAGCACAGCGGCCCTGGGCTGCCTGGTCAAGGACTA8180.11974066923030756No Hit
CACCAGGACTGGCTGAACGGCAAGGAGTACAAGTGCAAGGTCTCCAACAA8170.1195942869940847No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTC8000.1171057889782959No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC7920.11593473108851295No Hit
CTTGTACTCCTTGCCGTTCAGCCAGTCCTGGTGCACGACGGTGAGGACGCTGACCACACGGAACGTGCTGTTGAA7900.11564196661606721No Hit
CTCCTGTGCAGGCTCTGGATTCAGCTTCAGTAGTTATGAAATGAACTGGGTCCGCCAGGCTCCAGGGAAGGGG7880.11534920214362146No Hit
GTGTAGGTCTGGGTGCCGAAGTTGCTGGAGGTCACGGTCACCACGCTGCT7870.11520281990739861No Hit
GTGGGGTCCCGTCAAGGTTCAGTGGCAGTGGATTTGGGACAGATTTCACT7840.11476367319872999No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT7770.11373899754516989No Hit
CCTTCGGCCAAGGGACACGACTGGACATTAAACGAACTGTGGCTGCACCA7720.11300708636405556No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG7700.1127143218916098No Hit
CTCTCACACAGTAATAAACAGCCGTGTCGTCGGCGCTCAGGCTGTTCATTTGCAGATACAGAGAGTTCTGGGCGT7670.11227517518294121No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG7660.11212879294671832No Hit
GAGTGAAATCTGTCCCAAATCCACTGCCACTGAACCTTGACGGGACCCCACTTTGCAAATTGGATGCAGCATAGA7620.11154326400182686No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT7590.11110411729315824No Hit
GTGCTGGGTTTTCCTTGTTGCTATTTTAGAAGGTGTCCAGTGTGAGGTGCAGCTGGTGGAGTCTGGGGGAGGCT7580.11095773505693537No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATG7570.1108113528207125No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC7540.11037220611204389No Hit
GTCTCCAACAAAGGCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAAC7520.11007944163959815No Hit
CCGCTGGTCAGAGCGCCTGAGTTCCACGACACCGTCACCGGTTCGGGGAA7400.10832285480492372No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC7310.10700541467891789No Hit
CCTCCATGCCGTCCACGTACCAGTTGAACTGGACCTCGGGGTCTTCGTGGCTCACGTCCACCACCACGCACGTGA7300.10685903244269501No Hit
GTGTAAATGGTCTGACTTGCCCGGCAAGTGATGGTGACTCTGTCTCCTTCAGATGCAGACAGGGAGGATGGAGA7250.10612712126158066No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACA7240.1059807390253578No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC7230.10583435678913491No Hit
CGACTGGACATTAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAA7130.10437053442690623No Hit
GTGCAGCCACAGTTCGTTTAATGTCCAGTCGTGTCCCTTGGCCGAAGGTG7060.10334585877334614No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCA6990.10232118311978605No Hit
GTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCT6980.10217480088356318No Hit
GTCCTGGTGCACGACGGTGAGGACGCTGACCACACGGAACGTGCTGTTGAACTGCTCCTCCCGTGGCTTTGTCT6960.10188203641111743No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC6940.1015892719386717No Hit
GATCAGGAGCTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAATTTAAAT6940.1015892719386717No Hit
CCCTCATGATCTCCCGGACCCCTGAGGTCACGTGCGTGGTGGTGGACGTGAGCCACGAAGACCCCGAGGTCCAGT6930.10144288970244883No Hit
GGGTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGTTTTGGAGATGGTTTTCTCGATGGGGGCTGGGAGGCCTT6910.10115012523000308No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTG6900.10100374299378022No Hit
GTTCAACAGCACGTTCCGTGTGGTCAGCGTCCTCACCGTCGTGCACCAGG6850.10027183181266588No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA6840.100125449576443No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATGGTA851.1150405E-981.4390870
ATCGTAT208.836956E-568.4793539
CTATATC150.002451153568.3080835
TACGGAA150.002499408667.9730615
TCTGGAG9050.057.91366270
TATAGCT200.00722953151.9805252
TATATCG200.007630147551.2763236
TAACCGT200.007791321351.0059322
AAGTAGA1002.224415E-549.44515670
AACGGGG354.88942E-448.4404968
TGTTAGA6550.045.2932770
GAGCAAA8900.043.33395870
CTCTTAT804.0017767E-1142.4924931
AAGGGGG1900.040.87316569
GTATCAA12000.039.659661
AGCGCCG1050.00168326837.672570
GACAGGT1450.037.510621
AATAGTC501.371201E-436.3379566
TAACGGC400.002553580634.6149351
TTCGGGG8700.034.10010570
Produced by FastQC (version 0.11.5)