FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685243

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685243
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences683143
Sequences flagged as poor quality0
Sequence length18-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG24470.3581973320373626No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTA21730.3180885993122962No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCTGGGGAGCGGGGGCTTGCTGGCCGTG20940.30652440265068953No Hit
GAGTTACACTTCCCCGATCACCTTCGGCCAAGGGACACGACTGGACATTAAACGAACTGTGGCTGCACCAT20780.30418228687112364No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT20740.30359675792623214No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGTTTTGGAGATGGTTTT19690.28822662312283076No Hit
GTAATAAACAGCCGTGTCGTCGGCGCTCAGGCTGTTCATTTGCAGATACAGAGAGTTCTGGGCGTTGTCTCTGGA16270.2381638983346093No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT15900.23274775559436311No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC15050.22030526551541918No Hit
GTGTAACTCTGTTGACAGTAGTAGGTTGCAAAATCTTCAGGTTGCAGACCGCTGATGGTGAGAGTGAAATCTGTC14940.2186950609169676No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTT14130.20683809978291517No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG13750.2012755748064461No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGC13530.19805516560954295No Hit
GAGTACAAGTGCAAGGTCTCCAACAAAGGCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAACCAAAGGGCA13380.19585943206619988No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG12830.18780840907394206No Hit
GATCTATGCTGCATCCAATTTGCAAAGTGGGGTCCCGTCAAGGTTCAGTGGCAGTGGATTTGGGACAGATTTCA12800.18736926236527343No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGTTA12740.18649096894793624No Hit
ACCTACTACTGTCAACAGAGTTACACTTCCCCGATCACCTTCGGCCAAGGGACACGACTGGACATTAAACGAA11990.175512301231221No Hit
GTTTATTACTGTGTGAGAGAGACTAAATATACGACTCATAATGCTTTTGA11300.16541192693184298No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGACCTCCAGCAACTTCGGCACCCAGAC11270.16497278022317435No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAA11260.16482639798695148No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT11180.16365534009716853No Hit
GGACACGACTGGACATTAAACGAACTGTGGCTGCACCATCTGTCTTCATC11040.16160598879004837No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT11000.16102045984515687No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTC10910.15970301971915105No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCC10870.15911749077425957No Hit
GGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCTGGGGAGCGGGGGCTTGCTGGCCGTGGCACTCATTTA10810.15823919735692232No Hit
GAGTTGGGGCTGTGCTGGGTTTTCCTTGTTGCTATTTTAGAAGGTGTCCAGTGTGAGGTGCAGCTGGTGGAGT10550.15443325921512774No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGAGCGCCTGAGTTCCACGACACCGTCACCGGTTCGGG10410.15238390790800754No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT10400.15223752567178467No Hit
GTATGTGGTAATACCACTACCAGTAATGTAGGAAACCCACTCCAGCCCCTTCCCTGGAGCCTGGCGGACCCAGTT10360.1516519967268932No Hit
GTAGTAGGTTGCAAAATCTTCAGGTTGCAGACCGCTGATGGTGAGAGTGAAATCTGTCCCAAATCCACTGCCA9650.14125885795506943No Hit
GCATGGAGGTGCATAATGCCAAGACAAAGCCACGGGAGGAGCAGTTCAACAGCACGTTCCGTGTGGTCAGCGTC9620.14081971124640083No Hit
GGGTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGTTTTGGAGATGGTT9610.14067332901017796No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGA9420.13789206652194344No Hit
GATTTCACTCTCACCATCAGCGGTCTGCAACCTGAAGATTTTGCAACCTACTACTGTCAACAGAGTTACACTTCC9290.13598909745104612No Hit
GTGGTGGACGTGAGCCACGAAGACCCCGAGGTCCAGTTCAACTGGTACGT9170.13423251061637168No Hit
ACCACATACTACGCAGACTCTGTGAGGGGCCGATTCACCATCTCCAGAGACAACGCCCAGAACTCTCTGTATCT9100.1332078349628116No Hit
CTCCTGTGCAGGCTCTGGATTCAGCTTCAGTAGTTATGAAATGAACTGGGTCCGCCAGGCTCCAGGGAAGGG8790.12866998563990262No Hit
CATTTACCCGGAGACAGGGAGAGGCTCTTCTGTGTGTAGTGGTTGTGCAGAGCCTCATGCATCACGGAGCATGA8760.12823083893123402No Hit
CTACTACTGTCAACAGAGTTACACTTCCCCGATCACCTTCGGCCAAGGGACACGACTGGACATTAAACGAACTGT8710.12749892775011967No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACT8610.12603510538789095No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACA8550.12515681197055376No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT8540.12501042973433088No Hit
GTGGGGTCCCGTCAAGGTTCAGTGGCAGTGGATTTGGGACAGATTTCACTCTCACCATCAGCGGTCTGCAACCT8500.1244249007894394No Hit
GTCCTGGTGCACGACGGTGAGGACGCTGACCACACGGAACGTGCTGTTGAACTGCTCCTCCCGTGGCTTTGTCTT8450.12369298960832505No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT8380.12266831395476496No Hit
GACCTACACCTGCAACGTAGATCACAAGCCCAGCAACACCAAGGTGGACAAGACAGTTGAGCGCAAATGTTGTGT8360.12237554948231921No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT8310.12164363830120486No Hit
GCACTGAACACAGAGGACTCACCATGGAGTTGGGGCTGTGCTGGGTTTTC8290.12135087382875913No Hit
GTCTCCATCCTCCCTGTCTGCATCTGAAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGACCATTTACA8260.12091172712009053No Hit
GCATAGATCAGGAGCTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAATTTAAATAAGTGTAAATGGTCTGACTT8240.12061896264764478No Hit
GTGCTGGGTTTTCCTTGTTGCTATTTTAGAAGGTGTCCAGTGTGAGGTGCAGCTGGTGGAGTCTGGGGGAGGCTT8190.11988705146653043No Hit
CTCCTGATCTATGCTGCATCCAATTTGCAAAGTGGGGTCCCGTCAAGGTT8170.1195942869940847No Hit
GTCGTGTCCCTTGGCCGAAGGTGATCGGGGAAGTGTAACTCTGTTGACAGTAGTAGGTTGCAAAATCTTCAGGTT8120.11886237581297035No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGT8030.11754493568696453No Hit
GTGTAGTGGTTGTGCAGAGCCTCATGCATCACGGAGCATGAGAAGACGTT7930.11608111332473581No Hit
CTTGTACTCCTTGCCGTTCAGCCAGTCCTGGTGCACGACGGTGAGGACGCTGACCACACGGAACGTGCTGTTGAA7840.11476367319872999No Hit
CCTCCATGCCGTCCACGTACCAGTTGAACTGGACCTCGGGGTCTTCGTGGCTCACGTCCACCACCACGCACGT7770.11373899754516989No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG7740.11329985083650129No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC7720.11300708636405556No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA7540.11037220611204389No Hit
GTCTTCCCCCTGGCGCCCTGCTCCAGGAGCACCTCCGAGAGCACAGCGGCCCTGGGCTGCCTGGTCAAGGACTA7520.11007944163959815No Hit
GAGTGAAATCTGTCCCAAATCCACTGCCACTGAACCTTGACGGGACCCCACTTTGCAAATTGGATGCAGCATA7450.10905476598603805No Hit
GTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT7410.10846923704114658No Hit
CACCAGGACTGGCTGAACGGCAAGGAGTACAAGTGCAAGGTCTCCAACAA7400.10832285480492372No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCCCTGTCTGCATCTGAA7380.10803009033247797No Hit
GTGTAAATGGTCTGACTTGCCCGGCAAGTGATGGTGACTCTGTCTCCTTCAGATGCAGACAGGGAGGATGGA7260.10627350349780354No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTCGTGGAACTCAGGCGCTCTGACCAGC7080.10363862324579189No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTAATGTCCAGTCGTGTCCCTTGGCCGAAGGTGATCGGGGAAGT6920.10129650746622597No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT6850.10027183181266588No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATGGTA702.627894E-7104.36989670
CCTTTGT8200.094.44202470
AATCGAA600.001874730873.0589370
GTGTCAT200.00708060352.25483357
CAGCGTT200.00781744850.96274625
CGACATT200.00783560550.9328521
TACAACG200.00788798150.847095
TCCGGAA900.00933666548.7059570
GTATAGG352.5069274E-548.471931
ATGGTCA8000.045.6618370
GTGTCCA10700.043.698870
GAGCAAA12550.041.91428870
GACTGTA6950.037.84347570
AAACACA9200.036.52946570
GTATCAA11750.035.8073461
GTGTTCA2101.7652077E-434.78996770
TTGGTAT8550.034.17961570
CAATCGC400.002781128534.01759328
AGGTACA400.00283055933.8955762
CTACACA5108.0035534E-1131.51561770
Produced by FastQC (version 0.11.5)