FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685244

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685244
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences815137
Sequences flagged as poor quality0
Sequence length18-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTATTGTCAGCAGTATGCTATCTCCCCGCTCACTTTCGGCGGAGGGACCAAGGTTGAGATCAAAGGAACTG47050.5772035866363568No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC32560.39944205697937896No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTAT26570.3259574770866738No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA21790.2673170276898239No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG21190.2599563018241105No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC21110.2589748717086821No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG20800.25517183001139687No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC20490.25136878831411164No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG19160.23505251264511365No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCT18950.23247625859211396No Hit
CAATAATACACTGCAAAATCTTCAGGCTCCAGGGTGCTGATGGCTAGAGTGAAGTCTGTCCCAGACCCACTGCCA18470.2265876778995433No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG15970.19591798679240421No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT14610.17923367483012057No Hit
GTATGCTATCTCCCCGCTCACTTTCGGCGGAGGGACCAAGGTTGAGATCAAAGGAACTGTGGCTGCACCA14330.17579866942612102No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCC14320.17567599066169245No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTC13110.16083186016583717No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACTTCGCCCACAGCATCCTGACCGTGTC13090.16058650263698004No Hit
GTAATACACGGCCGTGTCCGCGGCGGTCACAGAGCTCAGCTTCAGGGAGAACTGGTTCTTGGACGCGTCTAATGA12570.15420720688669512No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA12470.15298041924240957No Hit
GTCTGGGACAGACTTCACTCTAGCCATCAGCACCCTGGAGCCTGAAGATTTTGCAGTGTATTATTGTCAGCAGTA12030.14758255360755307No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAAC11960.14672380225655318No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT11910.14611040843441042No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC11640.1427980817948394No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACT11480.14083522156398248No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC11390.1397311126841255No Hit
ATCTCACCCCGTTGACACGGTTAGTTTGCATGCACACACAGAGCGGCCAG10800.13249306558284069No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT10740.13175699299626933No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAGCAGCAACTACTTAGCCTGGTACC10440.12807663006341266No Hit
GTACAGGGTGGGTTTACCGGTGGACTTGTCCACGGTCCTCTCGGTGACCCTGTTGGGCAGGGCCTCATGGGCCAC10050.12329215825069896No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT9910.12157465554869917No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG9850.12083858296212784No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG9570.11740357755812827No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC9530.11691286250041404No Hit
GTGTATTATTGTCAGCAGTATGCTATCTCCCCGCTCACTTTCGGCGGAGGGACCAAGGTTGAGATCAAAGGAA9500.11654482620712836No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT9450.11593143238498559No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA9380.1150726810339857No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC9360.1148273235051286No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA9300.11409125091855724No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT9290.11396857215412869No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC9200.11286446327427167No Hit
GTCTTTGTCTCCAGGGGAGAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCAGCAACTACTTAGCC9190.11274178450984312No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC9050.11102428180784334No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGG8890.10906142157698644No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG8830.1083253489904151No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG8710.10685320381727244No Hit
GTCCCAGACCCACTGCCACTGAACCTCTCTGGGGTGCCAGTGGCCCTGCTGGATGCACCGTAGATGAGGAGCC8650.1061171312307011No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGGGGAGAGAGCCACCCTCTCCTGC8620.10574909493741542No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA8590.10538105864412975No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT8560.1050130223508441No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG8550.10489034358641554No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAGAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCAG8550.10489034358641554No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTT8540.10476766482198697No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCCTTTGATCTCAACCTTGGTCC8430.10341819841327285No Hit
CCCCAGAGAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCACTCTAGCCATCAGCACCCTGGAGCCTGAAGATT8420.1032955196488443No Hit
ACGTTGTACAGGGTGGGTTTACCGGTGGACTTGTCCACGGTCCTCTCGGT8320.10206873200455874No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC8250.10120998065355885No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGTAT150.002597479867.317561
GACCGGA609.757706E-567.10754470
AGTAAGA857.653396E-659.21253670
GGTATCA12100.049.2363971
ATGGCAA1704.307367E-947.3700370
GCGTAGA20900.047.20004370
TGTTAGA10500.046.9752870
AATGGTA1301.7334478E-646.4590770
TCGTATG1000.044.64124340
CGACAAA509.013156E-442.78498569
GTATCAA32050.040.432551
CTTATAC1500.040.3905371
TACACAT1600.039.9575465
CGTCTAT350.001491364738.62360828
TCTGTCG350.001524161738.453028
AGAGTCG451.1604413E-437.38488411
ATCTCGT1200.037.00181637
GGAATAA4150.036.38360670
CATGAAT1700.035.93196532
CGTATGC1250.035.76449241
Produced by FastQC (version 0.11.5)