FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685245

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685245
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences815137
Sequences flagged as poor quality0
Sequence length18-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTATTGTCAGCAGTATGCTATCTCCCCGCTCACTTTCGGCGGAGGGACCAAGGTTGAGATCAAAGGAACTGT42630.5229795727589349No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTA31170.3823897087238096No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT27930.3426417890489574No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC22250.27296025085353753No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGC21240.2605696956462533No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGTTA20570.25235021842954003No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTT19300.23677001534711342No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGGGTCAGTAGCAGGTGCCAGCTGTGTC18490.2268330354284004No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG17910.2197176670915441No Hit
CAATAATACACTGCAAAATCTTCAGGCTCCAGGGTGCTGATGGCTAGAGTGAAGTCTGTCCCAGACCCACTGCCA17080.20953532964397395No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGA16370.20082513736954646No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAA16300.19996638601854658No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT15210.18659440069583394No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG14520.17812956595026358No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT13580.1665977620939793No Hit
GTAATACACGGCCGTGTCCGCGGCGGTCACAGAGCTCAGCTTCAGGGAGAACTGGTTCTTGGACGCGTCTAAT13520.16586168950740796No Hit
GTATGCTATCTCCCCGCTCACTTTCGGCGGAGGGACCAAGGTTGAGATCAAAGGAACTGTGGCTGCACCAT13150.16132257522355137No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCC13030.1598504300504087No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT12700.15580203082426633No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTC12440.15261238294912388No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA12130.14880934125183864No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC12000.14721451731426743No Hit
GTCTGGGACAGACTTCACTCTAGCCATCAGCACCCTGGAGCCTGAAGATT11960.14672380225655318No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACTTCGCCCACAGCATCCTGACCGTGTC11720.14377951191026783No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACT11420.14009914897741116No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT11400.13985379144855406No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT10260.12586841230369863No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA10070.12353751577955609No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT10020.1229241219574133No Hit
ATCTCACCCCGTTGACACGGTTAGTTTGCATGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCA9940.12194269184198485No Hit
GTGTATTATTGTCAGCAGTATGCTATCTCCCCGCTCACTTTCGGCGGAGGGACCAAGGTTGAGATCAAAGGAA9870.12108394049098496No Hit
GTACAGGGTGGGTTTACCGGTGGACTTGTCCACGGTCCTCTCGGTGACCCTGTTGGGCAGGGCCTCATGGGCCAC9660.11850768643798529No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA9650.11838500767355671No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC9560.11728089879369973No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACA9560.11728089879369973No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA9390.11519535979841425No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCT9390.11519535979841425No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAGCAGCAACTACTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAG9040.11090160304341479No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC8970.1100428516924149No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGGTGAGGAAGATGCTGGCAAAGGATGG8780.10771195516827232No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTAC8760.10746659763941523No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC8630.10587177370184397No Hit
GTCTTTGTCTCCAGGGGAGAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCAGCAACTACTTAGCCTG8380.10280480459113008No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATA8320.10206873200455874No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTACGT404.0286104E-4107.5959570
TACGTAT509.780895E-486.0767670
GTTACTT600.002016913871.7306370
GTTATTA807.010295E-571.7306370
AATGGTA1907.2759576E-1267.9553470
ATGGCAA1803.074092E-1063.76056770
ATACGGC200.00812837450.46325315
GTCTCTT3350.047.10668670
GGAATAA4350.046.1714470
ACCATCA7850.045.688370
TAGGCAA4400.045.64676770
GAGTAAG307.161102E-444.83421312
GGTATCA13450.044.2861821
TTGCAAT3601.8189894E-1243.8353970
ACCGGTA7000.043.0383870
TACTGTT1400.001111315740.98893470
TATTCTA2104.097561E-640.98893470
CCGGGTT3853.6379788E-1240.9889370
GTATCAA34450.039.2693861
TTGCAAA1901.09226865E-437.75296470
Produced by FastQC (version 0.11.5)