FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004685246

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685246
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1270075
Sequences flagged as poor quality0
Sequence length19-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG45920.3615534515678208No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAG36270.2855736865933114No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCACGAAAGTGCTACTACCTGCATATGAG35110.27644036769482117No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGG34670.2729760053540145No Hit
CTATAGGAGAGCAAGCAGTAATACTCAGCCTCATCCTCAGGCTGCGCACCCGAAAGGGTCAGGGCAGCTTTGCCC31240.2459697261972718No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCACGAAAGTGCTA30100.2369938783142728No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC29190.22982894710942267No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC26430.20809794697163553No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCC25450.2003818672125662No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA25200.19841347951892607No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG24310.19140601932956716No Hit
GATTATTACTGCTGCTCATATGCAGGTAGTAGCACTTTCGTGGTATTCGG24020.1891226896049446No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCACTGGAACCAGCAGTGATGTT23600.18581579827962916No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA23490.1849497076944275No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGG22780.1793594866444895No Hit
CTCCTATAGTGGTGCTCGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCC22690.17865086707477904No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCGAGCACCACTATAGGAGAGCAAGCAG22650.17833592504379664No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG22640.17825718953605102No Hit
GTAATACAAGGCGGTGTCCTCAGTTCTCAGACTGTTCATTTGCAGATACA22170.17455662067200756No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG21760.17132846485443773No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCTGACGCCTGAGCAG21490.16920260614530638No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC21480.1691238706375608No Hit
CCACTATAGGAGAGCAAGCAGTAATACTCAGCCTCATCCTCAGGCTGCGCACCCGAAAGGGTCAGGGCAGCTT21320.16786410251363107No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC20250.15943940318485128No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGG20060.15794342853768478No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGT18920.14896758065468574No Hit
CCTATAGTGGTGCTCGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGG18770.14778654803850166No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCGAGCACCACTATAGGAGAGCAAGCAGTAATACTCAG18600.14644804440682638No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT18600.14644804440682638No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT18550.14605436686809833No Hit
GTAATAATCAGCCTCGTCCTCAGCCTGGAGCCCAGAGATTGTCAGGGAGG18420.14503080526740547No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA18320.1442434501899494No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC17930.1411727653878708No Hit
CTCCTGCACTGGAACCAGCAGTGATGTTGGGAGTTATAACCTTGTCTCCTGGTACCAACAGCACCCAGGCAAAGC17820.14030667480266912No Hit
GGCCTGGACTCCTCTCTTTCTGTTCCTCCTCACTTGCTGCCCAGGGTCCA17700.13936184870972185No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTT17690.13928311320197628No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG17650.13896817117099383No Hit
GTTATAACCTTGTCTCCTGGTACCAACAGCACCCAGGCAAAGCCCCCAAA17410.1370785189850993No Hit
CTCCTGGACACCTGCCCGGTTCTCAGGCTCCCTCCTTGGGGGCAAAGCTGCCCTGACCCTTTCGGGTGCGCAGC16890.1329842725823278No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG16760.13196071098163495No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGC16550.1303072653189772No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG16540.13022852981123162No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA16530.13014979430348603No Hit
GGACACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC16430.12936243922602994No Hit
TCCTCAGGCTGCGCACCCGAAAGGGTCAGGGCAGCTTTGCCCCCAAGGAGGGAGCCTGAGAACCGGGCAGGTGTC16420.12928370371828438No Hit
GTACTGGAGCTGTCACCAGTGGTCATTATCCCTACTGGTTCCAGCAGAAGGCTGGCCAAGCCCCCAGGACACTGA16320.1284963486408283No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA16150.127157845009153No Hit
GAGTATTACTGCTTGCTCTCCTATAGTGGTGCTCGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAG16040.12629175442395135No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCACGAAAGTGCTAC15840.12471704426903923No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT15730.12385095368383757No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAGTTGAGGGAGATGGGGTAGGTGGAGT15660.12329980512961833No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC15500.12204003700568863No Hit
CAGTAATACAAGGCGGTGTCCTCAGTTCTCAGACTGTTCATTTGCAGATACAGGGAGTTTTTGCTGTTGTCTC15480.12188256599019742No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG15350.12085900438950456No Hit
GTAATACTCAGCCTCATCCTCAGGCTGCGCACCCGAAAGGGTCAGGGCAG15160.11936302974233803No Hit
TTGTATTACTGTGCAAAAGATATTTCAGACGATTCTCGGGGGGAGGCTTTTGATATCTGGGGCCAAGGGACAA15100.11889061669586441No Hit
CAGTAATACTCAGCCTCATCCTCAGGCTGCGCACCCGAAAGGGTCAGGGCAGCTTTGCCCCCAAGGAGGGAGC15060.11857567466488199No Hit
GTATTACTGTGCAAAAGATATTTCAGACGATTCTCGGGGGGAGGCTTTTGATATCTGGGGCCAAGGGACAATGG15020.11826073263389958No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTG15020.11826073263389958No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC14910.11739464204869791No Hit
GACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCCTGCACTGGAACCAGCAGTGATGT14910.11739464204869791No Hit
GCCTGAGGATGAGGCTGAGTATTACTGCTTGCTCTCCTATAGTGGTGCTCGGGTGTTCGGCGGAGGGACCAAG14690.11566246087829458No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA14540.1144814282621105No Hit
GTCGATCACCATCTCCTGCACTGGAACCAGCAGTGATGTTGGGAGTTATAACCTTGTCTCCTGGTACCAACAGCA14460.11385154420014566No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT14400.11337913115367204No Hit
GTATTACTGCTTGCTCTCCTATAGTGGTGCTCGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCC14400.11337913115367204No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG14370.11314292463043521No Hit
GTCCAGGAGTGTTTGTTGCTTGTATCATAAATCAGTGTCCTGGGGGCTTG14350.112985453614944No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC14280.11243430506072477No Hit
TATCAGCACCATGGCCTGGACTCCTCTCTTTCTGTTCCTCCTCACTTGCTGCCCAGGGTCCAATTCCCAGGCTG14260.11227683404523355No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGG14230.11204062752199673No Hit
CTCCAGTACTGGAGCTGTCACCAGTGGTCATTATCCCTACTGGTTCCAGC14110.11109580142904946No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC14000.11022971084384779No Hit
GAGTTTGGACTGAGCTGGGTTTTCCTTGTTGCTATTTTAAAAGGTGTCCAGTGTGAAGTGCAGCTGGTGGAG13900.10944235576639175No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGC13870.10920614924315494No Hit
CTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCC13090.1030647796389977No Hit
GTCTGAGAACTGAGGACACCGCCTTGTATTACTGTGCAAAAGATATTTCA12970.10211995354605043No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC12950.10196248253055921No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCT12930.10180501151506802No Hit
CTCACTGACTGTGTCCCCAGGAGGGACAGTCACTCTCACCTGTGACTCCAGTACTGGAGCTGTCACCAGTGGTCA12900.1015688049918312No Hit
ACCATACACTGGGTCCGTCAAGCTCCGGGGAAGGGTCTGGAGTGGGTCTC12880.10141133397633997No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG12860.10125386296084876No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)