FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685247

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685247
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1270075
Sequences flagged as poor quality0
Sequence length18-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG46820.36863964726492526No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT34090.2684093459047694No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGT32760.25793752337460385No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCACGAAAGTGCTACTACCTGCATATGA31660.24927661752258723No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC30610.24100938920929865No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG27910.21975080211798517No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCA27560.21699505934688898No Hit
CTATAGGAGAGCAAGCAGTAATACTCAGCCTCATCCTCAGGCTGCGCACCCGAAAGGGTCAGGGCAGCTTTGCCC27470.2162864397771785No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC26870.2115623093124422No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCACGAAAGTGCTA26180.2061295592779954No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCCT24030.1892014251126902No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCACTGGAACCAGCAGTGATGTT23480.1848709721866819No Hit
GATTATTACTGCTGCTCATATGCAGGTAGTAGCACTTTCGTGGTATTCGGCGGAGGGACCAAGCTGACCGTCCTA23240.18298132000078735No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA22000.17321811704033227No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAG21660.17054110977698167No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCA21380.16833651556010473No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGT21100.16613192134322777No Hit
CTCCTATAGTGGTGCTCGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTC20790.163691120603114No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGG20160.15873078361514084No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTT19900.1566836604137551No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC19330.15219573647225557No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTG18980.14943999370115937No Hit
CCACTATAGGAGAGCAAGCAGTAATACTCAGCCTCATCCTCAGGCTGCGCACCCGAAAGGGTCAGGGCAGCTTT18970.14936125819341375No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCG18860.1484951676082121No Hit
GTAATACAAGGCGGTGTCCTCAGTTCTCAGACTGTTCATTTGCAGATACAGGGAGTTTTTGCTGTTGTCTCTGGA18800.14802275456173847No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC18280.143928508158967No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC17990.14164517843434443No Hit
CTCCTGCACTGGAACCAGCAGTGATGTTGGGAGTTATAACCTTGTCTCCTGGTACCAACAGCACCCAGGCAAAGC17890.14085782335688837No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAG17800.1401492037871779No Hit
GGCCTGGACTCCTCTCTTTCTGTTCCTCCTCACTTGCTGCCCAGGGTCCAATTCCCAGGCTGTGGTGACTCAGGA17730.13959805523295868No Hit
CTCCTGGACACCTGCCCGGTTCTCAGGCTCCCTCCTTGGGGGCAAAGCTGCCCTGACCCTTTCGGGTGCGCAGC17510.13786587406255535No Hit
CCTATAGTGGTGCTCGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTA17200.13542507332244158No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTT17190.135346337814696No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA17020.1340078341830207No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT17000.13385036316752946No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTA16850.1326693305513454No Hit
GGACACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC16750.13188197547388933No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAA16480.12975611676475798No Hit
GTTATAACCTTGTCTCCTGGTACCAACAGCACCCAGGCAAAGCCCCCAAACTCATGATTTATGAGGGCAGTAAGC16410.12920496821053876No Hit
GTACTGGAGCTGTCACCAGTGGTCATTATCCCTACTGGTTCCAGCAGAAGGCTGGCCAAGCCCCCAGGACACT15980.12581934137747772No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCGAGCACCACTATAGGAGAGCAAGCAGTAATACTCA15960.1256618703619865No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTG15610.1229061275908903No Hit
GTAATAATCAGCCTCGTCCTCAGCCTGGAGCCCAGAGATTGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCAGA15530.12227624352892545No Hit
GCCTGAGGATGAGGCTGAGTATTACTGCTTGCTCTCCTATAGTGGTGCTCGGGTGTTCGGCGGAGGGACCAA15330.12070153337401335No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC15310.12054406235852214No Hit
GAGTATTACTGCTTGCTCTCCTATAGTGGTGCTCGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCA15220.11983544278881168No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGC15050.1184969391571364No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG14970.11786705509517154No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTG14910.11739464204869791No Hit
GTATTACTGCTTGCTCTCCTATAGTGGTGCTCGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCC14740.11605613841702261No Hit
CTCCAGTACTGGAGCTGTCACCAGTGGTCATTATCCCTACTGGTTCCAGC14720.11589866740153142No Hit
TCCTCAGGCTGCGCACCCGAAAGGGTCAGGGCAGCTTTGCCCCCAAGGAGGGAGCCTGAGAACCGGGCAGGTGTC14690.11566246087829458No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATAC14630.11519004783182095No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTAT14530.11440269275436489No Hit
CAGTAATACTCAGCCTCATCCTCAGGCTGCGCACCCGAAAGGGTCAGGGCAGCTTTGCCCCCAAGGAGGGAGC14440.11369407318465444No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGG14410.11345786666141765No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC14410.11345786666141765No Hit
GAGTTTGGACTGAGCTGGGTTTTCCTTGTTGCTATTTTAAAAGGTGTCCAGTGTGAAGTGCAGCTGGTGGAGT14390.11330039564592642No Hit
CTCTTATTACTTGGGATGGTGGTAGCACATACTATGCAGACTCTGTGAAG14190.11172568549101432No Hit
GTATTACTGTGCAAAAGATATTTCAGACGATTCTCGGGGGGAGGCTTTTGATATCTGGGGCCAAGGGACAATGGT14120.11117453693679506No Hit
GTCTGAGAACTGAGGACACCGCCTTGTATTACTGTGCAAAAGATATTTCAGACGATTCTCGGGGGGAGGCTTTT14090.11093833041355826No Hit
GTCGATCACCATCTCCTGCACTGGAACCAGCAGTGATGTTGGGAGTTATAACCTTGTCTCCTGGTACCAACAGCA14070.11078085939806705No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC14010.1103084463515934No Hit
TATCAGCACCATGGCCTGGACTCCTCTCTTTCTGTTCCTCCTCACTTGCTGCCCAGGGTCCAATTCCCAGGCTGT13980.1100722398283566No Hit
TTGTATTACTGTGCAAAAGATATTTCAGACGATTCTCGGGGGGAGGCTTTTGATATCTGGGGCCAAGGGACAATG13800.10865500068893569No Hit
GACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCT13740.10818258764246207No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCACGAAAGTGCTAC13740.10818258764246207No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAG13620.1072377615495148No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGG13440.1058205224100939No Hit
CTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAG13410.10558431588685707No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCT13390.10542684487136586No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGG13320.10487569631714663No Hit
GTTCTCAGACTGTTCATTTGCAGATACAGGGAGTTTTTGCTGTTGTCTCTGGAGATGGTGAATCGGCCCTTCACA13200.10393087022419936No Hit
CAGTAATACAAGGCGGTGTCCTCAGTTCTCAGACTGTTCATTTGCAGATACAGGGAGTTTTTGCTGTTGTCTCTG13030.10259236659252408No Hit
GTAATACTCAGCCTCATCCTCAGGCTGCGCACCCGAAAGGGTCAGGGCAGCTTTGCCCCCAAGGAGGGAGCCTGA12980.10219868905379603No Hit
ACCATACACTGGGTCCGTCAAGCTCCGGGGAAGGGTCTGGAGTGGGTCTC12950.10196248253055921No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG12890.1014900694840856No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGACTTGCCGGCTAGGCACTGTGTGGCC12880.10141133397633997No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAG12840.10109639194535755No Hit
GGCCTGGGCTCTGCTGCTCCTCACTCTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGC12730.10023030136015589No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATGGTA2450.076.9065670
GCGATTT600.00193761672.4696570
AACTGTA1206.8107656E-660.39137370
GTGTAAA750.004691590657.97571670
AAGCCTT800.00605674754.35223470
AAAGTCA3100.051.43007370
GGTAGAA32300.050.70631870
TGACATT1204.9496494E-448.313170
GGTATCA9150.046.0430641
TGGTATC3100.043.7925222
ATGGCAA1756.342361E-541.4112370
GAGCCAT10500.040.03085770
GTATCAA23100.038.5347821
AAGCAGT17000.037.513770
TAGGCAA2409.5686955E-636.23482570
TTAAAGT2857.592753E-735.59912570
TTACTTT2101.8551524E-434.50935770
AGTGGAA18500.034.4720570
CCACGTT10150.034.2713670
GTTTGGT1700.002744531534.10336370
Produced by FastQC (version 0.11.5)