FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685248

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685248
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1327017
Sequences flagged as poor quality0
Sequence length18-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGATACCTCACCACTCACTTTCGGCGGGGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCA74620.5623138211492392No Hit
GTATCATACTGCTGACAGTAATATACTGCAAAATCTTCAGGCTCTAGTCTGGTGATGGTGAGAGTGAAGTCTGTC59260.44656549237877136No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTAT58750.4427222861500644No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC57810.43563872957166333No Hit
GTATATTACTGTCAGCAGTATGATACCTCACCACTCACTTTCGGCGGGGGGACCAAGGTGGAGATCAAACGAA46420.3498071237972083No Hit
ATATTACTGTCAGCAGTATGATACCTCACCACTCACTTTCGGCGGGGGGACCAAGGTGGAGATCAAACGAACTG46380.34950569585770186No Hit
GTAATATACTGCAAAATCTTCAGGCTCTAGTCTGGTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACCGCCAT39860.3003729417181543No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC39760.29961937186938825No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCT37110.2796497708770875No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA34200.25772088827799494No Hit
CAGTAATATACTGCAAAATCTTCAGGCTCTAGTCTGGTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACCGCCA33940.2557616066712032No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC33670.25372696807953476No Hit
GTCTGGGACAGACTTCACTCTCACCATCACCAGACTAGAGCCTGAAGATTTTGCAGTATATTACTGTCAGCAGTA33350.2513155445634834No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG32850.24754769531965304No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG31700.23888164205884324No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG31320.2360180766335322No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG31210.23518914979988953No Hit
GTCCCAGACCCACCGCCATTGAACCTGTCTGGGATGCCAGTGGCCCTGTTGGATGCATCATAGATGAGGAGTC31160.2348123648755065No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA30240.22787952226685868No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCC29520.222453819355743No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT27980.21084884368474557No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCGGGGCCAGTCAGAGTATTAGCAACAACTACTTAGCC27340.20602599665264276No Hit
GAGTATTAGCAACAACTACTTAGCCTGGTACCAGCAGAAACCTGGCCTGG26200.1974353003767096No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAAC24480.1844738989779332No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACT24310.1831928302350309No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT23140.17437606300446792No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT22090.16646357959242422No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGA21760.1639767990914962No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAC19690.14837790322203862No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG19680.148302546237162No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC19430.14641862161524682No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC19030.14340434222018256No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC18910.1425000584016633No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA18900.14242470141678668No Hit
CCATTGAACCTGTCTGGGATGCCAGTGGCCCTGTTGGATGCATCATAGATGAGGAGTCTGGGCGCCAGGCCAGGT18670.14069149076462473No Hit
GTCTCCCGCCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCGGGGCCAGTCAGAGTATTAGCAA18670.14069149076462473No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT18550.13978720694610544No Hit
GAGTGAAGTCTGTCCCAGACCCACCGCCATTGAACCTGTCTGGGATGCCAGTGGCCCTGTTGGATGCATCATAGA18180.136998998505671No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCCCCGCCGAAAGTG17510.13195008051893833No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG17270.1301415128818998No Hit
GACTTCACTCTCACCATCACCAGACTAGAGCCTGAAGATTTTGCAGTATATTACTGTCAGCAGTATGATACCTCA17130.12908651509362729No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA16760.12629830665319286No Hit
TCTATGATGCATCCAACAGGGCCACTGGCATCCCAGACAGGTTCAATGGCGGTGGGTCTGGGACAGACTTCAC16390.12351009821275839No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC16270.12260581439423911No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCA16160.12177688756059644No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATGGGTGGGTTTACCCGCCAAGCGG16010.12064653278744733No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG15920.11996831992355787No Hit
CTCCTGCGGGGCCAGTCAGAGTATTAGCAACAACTACTTAGCCTGGTACC15900.11981760595380465No Hit
GTATTAGCAACAACTACTTAGCCTGGTACCAGCAGAAACCTGGCCTGGCGCCCAGACTCCTCATCTATGATGCA15780.11891332213528538No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTT15770.11883796515040877No Hit
CCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCACCGGAGAAATTGTGTTGACGCAGTCTC15670.1180843953016427No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCC15660.1180090383167661No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT15270.11507011590657845No Hit
CTGCTACTCTGGCTCCCAGATACCACCGGAGAAATTGTGTTGACGCAGTC15170.11431654605781236No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT14930.11250797842077383No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC14660.11047333982910543No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGG14500.10926762807107972No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT14460.10896620013157331No Hit
CAATAATACACGGCCGTGTCGGCAGATCTCAGGTTGCCCAGCTCCATGTAGGCTGTGCTGATGGACCTGTCCC14380.10836334425256043No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC14270.10753441741891775No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA14170.1067808475701517No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTG14100.10625334867601546No Hit
ACCCTCTCCTGCGGGGCCAGTCAGAGTATTAGCAACAACTACTTAGCCTGGTACCAGCAGAAACCTGGCCTGGCG14070.10602727772138563No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTT13960.10519835088774296No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT13860.10444478103897689No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC13840.10429406706922367No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG13810.10406799611459386No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC13480.10158121561366583No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC13440.10127978767415942No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA13440.10127978767415942No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATGGTA4600.046.5952770
AGGCTCG906.756099E-445.3625970
TGTTAGA14150.044.72142470
GTATCAA30150.039.870871
ACGAGAC2650.037.1627922
GAGCAAA26000.036.90072370
ACATGCG2700.036.50761827
GCGTAGA31250.035.6005670
CGTATGC2700.035.57897641
TATCAAC34300.034.9333572
CAGTTCG20300.034.69232670
TCGTATG2800.034.2678440
ATGCCGT2950.033.80662544
ATCAACG35400.033.751973
TCAACGC35650.033.5165444
CGACCGG3050.033.38603211
TCTCGCG10100.033.34824470
AACGCAG35950.033.3312726
CAACGCA35900.033.2831425
CACGAGA3000.032.8271321
Produced by FastQC (version 0.11.5)