FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685249

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685249
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1327017
Sequences flagged as poor quality0
Sequence length18-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGATACCTCACCACTCACTTTCGGCGGGGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT68010.5125028541458022No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT57440.43285052113122896No Hit
GTATCATACTGCTGACAGTAATATACTGCAAAATCTTCAGGCTCTAGTCTGGTGATGGTGAGAGTGAAGTCTGTC57410.43262445017659906No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTA56470.4255408935981981No Hit
GTATATTACTGTCAGCAGTATGATACCTCACCACTCACTTTCGGCGGGGGGACCAAGGTGGAGATCAAACGAA44310.3339067999882443No Hit
ATATTACTGTCAGCAGTATGATACCTCACCACTCACTTTCGGCGGGGGGACCAAGGTGGAGATCAAACGAACTGT43250.325918959591324No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC37630.283568334090671No Hit
GTAATATACTGCAAAATCTTCAGGCTCTAGTCTGGTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACCGCCATT37370.28160905248387924No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTT36200.2727922852533163No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGC35780.2696272918884988No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA33520.2525966133063857No Hit
GTCTGGGACAGACTTCACTCTCACCATCACCAGACTAGAGCCTGAAGATT32700.2464173405465039No Hit
CAGTAATATACTGCAAAATCTTCAGGCTCTAGTCTGGTGATGGTGAGAGT32380.24400591703045255No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG30880.23270236929896151No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT29740.22411167302302834No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGTTA29390.2214741785523471No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG28710.21634990358073786No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCC28100.21175312750326483No Hit
GTCCCAGACCCACCGCCATTGAACCTGTCTGGGATGCCAGTGGCCCTGTTGGATGCATCATAGATGAGGAGTCTG27420.20662885253165558No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAA27360.20617671062239593No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCGGGGCCAGTCAGAGTATTAGCAACAACTACTTAGCCTG27040.20376528710634453No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTC26700.2012031496205399No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGA25310.19072852872269158No Hit
GAGTATTAGCAACAACTACTTAGCCTGGTACCAGCAGAAACCTGGCCTGG25270.19042710078318514No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT24840.18718675043349106No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT24170.1821378324467584No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT22880.17241678139767613No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACT22350.16842286119921598No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCA22140.16684036451680725No Hit
CTCCTGCGGGGCCAGTCAGAGTATTAGCAACAACTACTTAGCCTGGTACCAGCAGAAACCTGGCCTGGCGCCCA21810.1643535840158792No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA19270.14521290985722113No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACA19190.14461005397820825No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC18980.14302755729579952No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT18850.14204791649240364No Hit
CCATTGAACCTGTCTGGGATGCCAGTGGCCCTGTTGGATGCATCATAGAT18010.1357179297627687No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAC17960.13534114483838564No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCCCCGCCGAAAGTG17910.1349643599140026No Hit
GAGTGAAGTCTGTCCCAGACCCACCGCCATTGAACCTGTCTGGGATGCCAGTGGCCCTGTTGGATGCATCATA17400.13112115368529567No Hit
GACTTCACTCTCACCATCACCAGACTAGAGCCTGAAGATTTTGCAGTATATTACTGTCAGCAGTATGATACCTCA17370.13089508273066583No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCT17350.13074436876091264No Hit
GTCTCCCGCCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCGGGGCCAGTCAGAGTATTAGCAA17270.1301415128818998No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA16400.123585455197635No Hit
ATTATATACACTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGATGGATCAACCCTCACAGTGGT16370.12335938424300517No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC16340.12313331328837535No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG16280.12268117137911572No Hit
CTGCTACTCTGGCTCCCAGATACCACCGGAGAAATTGTGTTGACGCAGTCTCCCGCCACCCTGTCTTTGTCTCCA16100.12132474565133679No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT16060.12102331771183038No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT15380.11589904274022111No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTA15030.11326154826953988No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGG14960.11273404937540363No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA14910.11235726445102061No Hit
CCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCACCGGAGAAATTGTGTTGACGCAGTCTC14880.11213119349639078No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTAC14560.10971976998033937No Hit
TCTATGATGCATCCAACAGGGCCACTGGCATCCCAGACAGGTTCAATGGCGGTGGGTCTGGGACAGACTTCACT14520.10941834204083294No Hit
ACCCTCTCCTGCGGGGCCAGTCAGAGTATTAGCAACAACTACTTAGCCTGGTACCAGCAGAAACCTGGCCTGGC14230.10723298947941134No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAA14000.1054997788272494No Hit
GTATTAGCAACAACTACTTAGCCTGGTACCAGCAGAAACCTGGCCTGGCGCCCAGACTCCTCATCTATGATGCAT13990.10542442184237279No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA13830.10421871008434708No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA13740.10354049722045762No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT13650.10286228435656816No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG13550.1021087145078021No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGGTT200.007602248141.6463370
GTAGTTT1403.3742253E-970.8231670
AATGGTA4050.055.95903870
CGGAGTT306.885339E-445.1951322
GAGCAAA30350.043.8706970
ATCGCAT400.001079505541.24901268
TATCGCT350.001258878639.9802458
GTATCAA27250.039.404951
CAGCCAT14350.037.5091370
TAAGTCA1901.179027E-437.27535270
AAGCGGT11250.035.25419670
AAGGACA30850.034.43589870
TATCAAC31000.034.396342
ATCAACG32600.032.6018833
CAACTTT3051.5446058E-632.5089970
TCAACGC33450.031.9758114
CAACGCA34100.031.3674855
AACGCAG34700.030.8262716
TATTCTA1850.00464759530.62623470
CCAGGTT20650.030.181370
Produced by FastQC (version 0.11.5)