FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685250

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685250
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1042591
Sequences flagged as poor quality0
Sequence length18-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTGTACCTTGCTCGCAGTAATAAACCCCAACATCCTCAGCCTCCACCCTGCTGATTTTCAGTGTGAAGTCAG54690.5245585277448204No Hit
GGTACACACTGGCCGCACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA50580.4851375083805634No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTAT43150.41387274588021566No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA41700.3999650869804171No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC41420.39727947008942144No Hit
GTAATACACAGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATATA38760.3717661096249632No Hit
CCCTTGGACAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAAAGCCTCGTA38730.3714783649580708No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC28980.27796134821804525No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCT28720.2754675611049779No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG24760.2374852650751829No Hit
GGGTTAGGCACTGACTTCACACTGAAAATCAGCAGGGTGGAGGCTGAGGA24680.23671794596346984No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC24480.23479964818418725No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCC23060.221179733951281No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA22920.2198369255057832No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG22350.21436977683482786No Hit
GTGTATTACTGTGCGAGGGACCCCTTCTATGGCTCTGTTGACTACTGGGGCCAGGGAACCCTGGTCACCGTCTC22350.21436977683482786No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG22040.21139641527693986No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG21720.20832713883008774No Hit
GGCCAAAAGTGTGCGGCCAGTGTGTACCTTGCTCGCAGTAATAAACCCCAACATCCTCAGCCTCCACCCTGCTGA21290.2042027986046302No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTC21280.20410688371566604No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT20900.2004621179350292No Hit
AGGTACACACTGGCCGCACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA20410.19576228837578685No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT19480.18684220370212287No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAAC19250.18463616125594792No Hit
GTCCAAGGGTGACGGGCAGGGAGAGTGGAGACTGAGTCATCACAACATCCCCACTGGATCCTGGGACCCAGAGCA18370.1761956510271046No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACT17990.1725508852464677No Hit
GTCCCAGACAGATTCAGCGGCAGTGGGTTAGGCACTGACTTCACACTGAAAATCAGCAGGGTGGAGGCTGAGGA17920.1718794810237188No Hit
GTAATAAACCCCAACATCCTCAGCCTCCACCCTGCTGATTTTCAGTGTGAAGTCAGTGCCTAACCCACTGCCG17380.16670007701965583No Hit
GGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGG16970.16276756657212654No Hit
GTCTGGGACCCCAGAGTCCCGGTTAAAAACGTTATAAATTAGGCGCCTTGGAGATTGGCCTGGCCTCTGCTGAAA16520.15845139656874077No Hit
GGCCAGGCCAATCTCCAAGGCGCCTAATTTATAACGTTTTTAACCGGGAC16010.1535597372315702No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT15360.1473252694489018No Hit
GCTCCTGGGGCTGCTAATGCTCTGGGTCCCAGGATCCAGTGGGGATGTTGTGATGACTCAGTCTCCACTCTCC15100.14483148233583445No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC15000.14387233344619318No Hit
GCTAATGCTCTGGGTCCCAGGATCCAGTGGGGATGTTGTGATGACTCAGTCTCCACTCTCCCTGCCCGTCACCCT14740.14137854633312585No Hit
GTCTCCACTCTCCCTGCCCGTCACCCTTGGACAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAAAGCCTCGTAAA14520.139268418775915No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG14470.13878884433109437No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTG14360.13773378055248894No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA14100.13523999343942159No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC14070.1349522487725292No Hit
CTCCCTGCCCGTCACCCTTGGACAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAAAGCCTCGTAAACGGTGA13890.1332257807711749No Hit
CAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCCCAGGATCCAGTGGGGATGTTGTGATGA13840.13274620632635423No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT13770.13207480210360534No Hit
CCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTG13650.1309238234360358No Hit
CAGTAATACACAGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATATAGTGAGTTCTTCGCGTTGTCTC13570.13015650432432277No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13490.12938918521260975No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG13400.12852595121193258No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA13270.1272790576553989No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTGTGCGGCCAGTG13120.12584033432093697No Hit
GGTCTAGTCAAAGCCTCGTAAACGGTGATGGAAACACGTACTTGCATTGGTTTCAGCAGAGGCCAGGCCAATCTC12770.12248331320719247No Hit
GATCAGCACTGCGCACAGAGGACTCACCATGGAGTTGGGGCTGAACTGGGTTTTCCTTGTTGCTATTTTAGAAGG12560.12046910053894577No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG12390.11883854742655559No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA12210.11711207942520126No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC12160.1166325049803806No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT11830.11346731364456435No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATGGGTGGGTTTACCCGCCAAGCGG11810.1132754838666361No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTT11690.11212450519906655No Hit
GGTTTCAGCAGAGGCCAGGCCAATCTCCAAGGCGCCTAATTTATAACGTT11620.11145310097631766No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTTAGTAGCTCTTGGATGGGCTGGGTCCGCCAGGCTCCAGGGAAAGGG11620.11145310097631766No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTAGGTGGGAAGTTTCTGGCGGT11610.11135718608735352No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCA11590.11116535630942527No Hit
GCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCCCAGGATCCAGTGGGGATGTTGTGATGACTCAGTCTCCAC11290.1082879096405014No Hit
GTATTACTGTGCGAGGGACCCCTTCTATGGCTCTGTTGACTACTGGGGCC11140.10684918630603948No Hit
GTACACACTGGCCGCACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCT11000.10550637786054166No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC10990.10541046297157754No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA10880.10435539919297213No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA10830.10387582474815148No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAGTGTCCTGCCTGGCTGTGCCCAGCCA10820.10377990985918735No Hit
GTGCAGCGACAGTCGGGGGTGGCAGCATGGGGGAGGTGGGGGAACTGGGC10700.10262893119161781No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT10610.10176569719094065No Hit
CAGTAATAAACCCCAACATCCTCAGCCTCCACCCTGCTGATTTTCAGTGTGAAGTCAGTGCCTAACCCACTGCCG10450.1002310589675146No Hit
TTATTACTGCGAGCAAGGTACACACTGGCCGCACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTG10440.10013514407855045No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGGAG11300.077.1157470
CCTACGA400.001839011173.4332470
CTCTTAT700.053.1198771
TATATCG200.00798576350.690745
GGTATCA8650.046.1135251
ACTTGCA18000.045.1478570
CATCTGA20050.044.43840470
TCTTATA851.8189894E-1243.7289922
TGTTAGA10250.041.07485670
GTGGTAT1950.038.1373441
GTATCAA21800.037.9902341
TGGTATC2100.037.0087362
TCTCGCG7450.036.79876370
AACTGTA1351.3866773E-436.26333270
AATGGTA3301.8189894E-1235.60399670
GTGGCCG9700.035.3287170
TAAGCCC400.00259417734.5054846
GGACTTA601.5466285E-533.79382714
TCAACGC26450.031.4301764
ATCAACG26450.031.4286693
Produced by FastQC (version 0.11.5)